[PDF] Top 20 Analysis of Mason-Pfizer Monkey Virus Gag Particles by Scanning Transmission Electron Microscopy

... retroviral Gag polyproteins associate with one another to enclose the RNA viral genome within a spherical particle (an immature capsid) with an electron-dense rim and a lucent center ...complete Gag ... See full document

... a transmission electron microscope, can be well carried out by low-keV STEM in a modern scanning electron microscope equipped with STEM detector, on-axis CCD camera, and a double-tilt specimen ... See full document

... STEVEN'* Laboratory of Physical Biology, National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases1 and Division of Computer Research and Technology,4 National Institutes [r] ... See full document

... RCASBP-AP virus preps in VSB were used to prepare grids for ...dark-field electron micrograph is shown in ...between particles), and using TMV on the same grid as a microscope calibrator ... See full document

... Scanning transmission electron microscopy (STEM) has advanced rapidly in the last decade thanks to the ability to correct the major aberrations of the probe-forming ...and analysis in ... See full document

... the Gag and Gag-Pro precursor proteins of M-PMV assemble into immature capsid structures (23) in an ATP-dependent manner (32) upon synthesis in an in vitro ...gradient analysis of translation ... See full document

... cryoelectron microscopy followed by image ...in virus cores, while the M-PMV ⌬p4Gag and ...microscopic analysis of MuLV immature and mature viruses, Yeager et ...immature particles and 115 Å ... See full document

... The Gag polyprotein of retroviruses drives immature virus assembly by forming hexameric protein ...cryoelectron microscopy analysis of immature Mason-Pfizer monkey ... See full document

... Mutational analysis of different retroviral species, such as al- pharetroviruses ...sarcoma virus [RSV]), lentiviruses ...leukemia virus [MLV]), and betaretroviruses (e.g., Mason-Pfizer ... See full document

... of Gag, has been previously shown to be sufficient for assembly of immature, spherical VLPs in bacte- rial cells and in vitro (41, 46, ...immature particles. This approach elimi- nated any interference from ... See full document

... a CTE binding domain (approximately residues 80 to 372) and a transportin-dependent nuclear localization signal (NLS; res- idues 61 to 102) (1, 2, 6, 19, 20, 32). A third domain, extending from approximately residue 540 ... See full document

... To identify the glycoproteins of MPMV, virus grown in CMMT was separately labeled with [IHI glucosamine and ["C Jamino acids and copurified by two density centrifugations and one velocit[r] ... See full document

... tubular particles, since it lacks the ability to form the N- terminal proline-aspartate salt bridge that has been re- ported to be the driving force for tube formation ...tubular particles (of a length ... See full document

... M-PMV particles detected with rabbit antibodies raised against M-PMV ...the virus was filtered from culture media and normalized by quantitative Western blotting for ...(E) Electron micrographs of ... See full document

... We tested the effects of these mutations on the fusion ki- netics of the glycoprotein in time course experiments. In order to determine the fusion kinetics at early time points, we used a fluorescent dye redistribution ... See full document

... 0 ase was confirmed as the site of action of these inhibitors because virions produced from transfected COS-1 cells in the presence of 100 p.M concentrations of inhibitor had the same pr[r] ... See full document

... the virus, whereas the TM glycoprotein anchors the SU protein at the surface of infected cells or the viral membrane ...mediates virus-cell membrane fusion during viral entry, as well as cell-cell fusion, ... See full document

... into virus, the mutated env genes were cloned into an infectious molecular clone, ...(B) Virus-containing supernatants from metabolically labeled COS-1 cells transfected with either wild-type or mutant ... See full document

... incident electron by the spec- imen atomic nucleus or by outer shell electrons of similar ...the electron to the ...an electron beam strikes a sample, including the emis- sion of characteristic ... See full document

... The glycoprotein precursor of M-PMV, Pr86, is cleaved by a cellular Golgi endopeptidase to yield the mature gp7O SU and gp22 TM proteins, and then, following virus release, a viral prote[r] ... See full document