[PDF] Top 20 The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes
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The Assembly-Activating Protein Promotes Capsid Assembly of Different Adeno-Associated Virus Serotypes
... AAP-5 protein obtained by PCR ...with different 5 ⬘ -untranslated extensions (data not shown) were completely sequenced excluding point mutations or deletions leading to reduced protein ...the ... See full document
12
Mapping and Engineering Functional Domains of the Assembly-Activating Protein of Adeno-associated Viruses
... AAV capsid assembly and the determinants of serotype ...restore capsid assembly (to ...residues different between 1CC and 5CC (T44M and Q50R), it is not surprising that the 5CC ... See full document
18
Surface Loop Dynamics in Adeno-Associated Virus Capsid Assembly
... the adeno-associated virus (AAV) capsid surface that extends from each viral protein (VP) subunit overlapping the neighboring fivefold ...AAV serotypes. In order to understand ... See full document
12
Capsid protein expression and adeno-associated virus like particles assembly in Saccharomyces cerevisiae
... 4 different time points, cells were collected and subjected to protein ...Cap protein ex- pression was analyzed by Western blot analysis of total cell lysates at each of these time points (Figure ... See full document
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Identification and Characterization of Nuclear and Nucleolar Localization Signals in the Adeno-Associated Virus Serotype 2 Assembly-Activating Protein
... small protein that can passively cross the nuclear envelope through the nuclear pore com- ...AAP2 protein during the course of AAV2 particle production in HEK 293 cells by plasmid transfection, where AAP2 ... See full document
11
Relevance of Assembly-Activating Protein for Adeno-associated Virus Vector Production and Capsid Protein Stability in Mammalian and Insect Cells
... FIG 5 Broad interchangeability of AAP variants from multiple AAV serotypes. (A) Western blot analysis to confirm proper AAP expression from all constructs. HEK293T cells were transfected with the expression ... See full document
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Subcellular compartmentalization of adeno-associated virus type 2 assembly.
... and capsid proteins colocalize in the nuclei of infected ...fluorescent capsid regions where they did not see an increase in Rep ...detect capsid protein ...and capsid proteins could be ... See full document
12
Comparative Analysis of Adeno-Associated Virus Capsid Stability and Dynamics
... capsid’s protein content. The less abundant capsid proteins, VP1 (⬃87 kDa) and VP2 ( ⬃ 73 kDa), containing unique N-terminal exten- sions (VP1u) containing a phospholipase A2 (PLA2) domain and nuclear ... See full document
11
Residues on Adeno-associated Virus Capsid Lumen Dictate Interactions and Compatibility with the Assembly-Activating Protein
... 3 Capsid lumenal surface charge influences AAP-VP assembly ...total protein per lane), and VP protein detected by Western blotting with the B1 ... See full document
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Adeno-associated Virus (AAV) Assembly-Activating Protein Is Not an Essential Requirement for Capsid Assembly of AAV Serotypes 4, 5, and 11
... each different DNA barcode contained in each pdsAAV-U6-VBCx plasmid) are all double-stranded AAV vector plasmids carrying a 135-bp DNA fragment (nucleotide positions 4445 to 4579 of pAAV9-SBBANN-VBCLib [GenBank ... See full document
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Properties of the Adeno-Associated Virus Assembly-Activating Protein
... first assembly-negative deletion mutant construct from amino acid position 28, which are completely conserved among the se- rotypes ...are different, with two prolines and a tryptophan (P43, W46, and P49) ... See full document
11
Context-Dependent Cleavage of the Capsid Protein by the West Nile Virus Protease Modulates the Efficiency of Virus Assembly
... C protein variants with single amino acid substitu- tions (at the P4, P3, P2, or P2= position) to determine if individual substitutions influence WNV NS2B/3 ...C protein cleavage detected for NGC mutant ... See full document
11
Feline Calicivirus Capsid Protein Expression and Capsid Assembly in Cultured Feline Cells
... authentic virus particles when tested in Western blots (data not shown). Capsid assembly has been recently described for FCV ...precursor capsid protein gene was expressed in a baculo- ... See full document
5
Assembly of the Herpes Simplex Virus Capsid: Preformed Triplexes Bind to the Nascent Capsid
... the capsid proteins (VP5, pre-VP22a, and VP23) and one of the VP19C deletion ...scaffolding protein, was visible in each ...that assembly with this mutant is much less efficient than with the ... See full document
8
Expression and Self-Assembly of Norwalk Virus Capsid Protein from Venezuelan Equine Encephalitis Virus Replicons
... immunodeficiency virus type 1 immunogen FIG. 5. Posttranslational processing of rNV capsid proteins synthesized in mammalian ...untreated capsid proteins were added to the reaction mixtures and the ... See full document
8
Essential Elements of the Capsid Protein for Self-Assembly into Empty Virus-Like Particles of Hepatitis E Virus
... binant capsid protein was observed when aa residues 112 to 660 of ORF2 were expressed with a recombinant baculovirus in two insect cell lines, Tn5 and ...53K protein was found in the culture me- ... See full document
8
Human cytomegalovirus capsid assembly protein precursor (pUL80.5) interacts with itself and with the major capsid protein (pUL86) through two different domains.
... the assembly pathway of herpes group viruses have resulted in the identification and charac- terization of multiple particle forms ...abundant capsid form ... See full document
12
A Small Loop in the Capsid Protein of Moloney Murine Leukemia Virus Controls Assembly of Spherical Cores
... core assembly and viral ...from different retroviruses, their secondary and tertiary structures appear to be remarkably well conserved (7, ...sarcoma virus [RSV] and HIV-1) suggest that CTDs play an ... See full document
10
Identification of a Varicella-Zoster Virus Replication Inhibitor That Blocks Capsid Assembly by Interacting with the Floor Domain of the Major Capsid Protein
... anti-varicella-zoster virus compound, a derivative of pyrazolo[1,5-c]1,3,5-triazin-4-one (coded as 35B2), was identified from a library of 9,600 random ...Twenty-four virus clones resistant to 35B2 were ... See full document
10
Sequence Properties of the MAAP Protein and of the VP1 Capsid Protein of Adeno-Associated Viruses
... the capsid located upstream of VP3 (ie VP1u and VP2c, see Fig ...between capsid subunits. Upon virus entry in cells and endocytosis, VP1u and VP2c are located within the capsid, but are ... See full document
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