[PDF] Top 20 Association of Nef with the Human Immunodeficiency Virus Type 1 Core

... protein Nef is essential for efficient replica- tion and pathogenesis of primate ...the nef open reading frame results in attenuation of simian immunodeficiency virus in rhesus macaques, and ... See full document

... of Nef in virus replication and pathogenesis (23, 25). In vitro, Nef has been shown to have at least two independent functions: down- regulation of cell surface CD4 and enhancement of viral in- ... See full document

... understand Nef interactions with Pak2, we performed mutational analysis of three primary HIV-1 Nef clones that exhibited similar capacities for downregulation of CD4 and MHC-I but variable abilities ... See full document

... LAI Nef immunoprecipitated from cell ...ELI Nef protein species was detected ...LAI Nef and ELI Nef, respectively (see ...particle-associated Nef were com- pared by scanning ... See full document

... that Nef does not affect the process of HIV-1 core disassembly ...HIV-1 core disassembly in vitro. Purified HIV-1 cores were incubated at 37°C for the indicated times, followed ... See full document

... membrane association) is crit- ical to the virion modification induced by Nef ...that Nef is a virion protein. Data presented herein indicate that Nef is indeed a virion ...virion-associated ... See full document

... lentiviral Nef is not essential for viral replication, it dramatically influences the clinical outcome of infection of macaques by simian immunodeficiency virus and possibly of humans by human ... See full document

... from virus producer cells (data not ...from nef-defective virions exhibited decreased amounts of all three of the major phosphoserine-specific pep- tides ...contrast, Nef did not influence the ... See full document

... The Nef protein of human immunodeficiency virus type 1 (HIV-1) stimulates viral ...disrupted nef genes were 4 to 40 times less infectious than wild-type ... See full document

... virion core is likely to be a target for Vif ...during virus assembly, or their processing during virus matu- ...the core other than a Gag or Pol protein (2, 33, 62) may be a target for Vif ... See full document

... lacking Nef. As shown in Fig. 3, the infectivity of nef-positive CD4-chemokine receptor pseudotypes for HeLa cells expressing HIV-1 Env was slightly reduced when infections were carried out in the ... See full document

... the nef gene (2). This suggests that Nef has an effect on replication which is independent of CyPA incorpora- ...tion. Nef has many effects on replication and pathogenicity, and it is possible that ... See full document

... of nef on the immunogenicity and pathoge- nicity of HIV-1 infection suggests that this gene could be a target for enhancing cellular immunity in chronically infected individuals or the design of ... See full document

... with human immunodeficiency virus type 1 (HIV-1), the majority of patients experience an extended asymptomatic period after seroconversion and prior to the development of AIDS, ... See full document

... of Nef change during the course of HIV-1 ...mary nef alleles obtained before and after disease progression are summarized in ...Consensus Nef proteins containing features that were more ... See full document

... in nef are rarely present in HIV-1 ...in nef in about 200 HIV-1-infected ...in nef were detected in only one subject ...(Table 1). One LTNP with a high frequency of defective ... See full document

... of Nef 1 and Nef 2 isogenic human immunodeficiency virus in CEM, HUT78, MT4 lymphoid, and U937 monocytic cell ...the virus particles released in culture media by measuring ... See full document

... SIV Nef and HIV-1 Nef can bind directly to both Lck and ...SIV Nef and Lck is ...the Nef proteins of HIV-1 and SIV. Unlike the case for its HIV-1 counterpart, significant ... See full document

... HIV-1 virus-like particles (VLPs) bearing the viral protease ...disrupt core stability and integrity decrease the ability of VLPs to abrogate HIV-1 restriction in OMK cells ...retroviral ... See full document

... The Nef protein of the type 1 human immunodeficiency virus (HIV-1) plays a key although poorly understood role in accelerating the progression of clinical disease in ... See full document