[PDF] Top 20 EWSR1 Binds the Hepatitis C Virus cis-Acting Replication Element and Is Required for Efficient Viral Replication

... Is Required for Efficient Viral Replication Todd ...The hepatitis C virus (HCV) genome contains numerous RNA elements that are required for its ...the ... See full document

... the replication of RNA ...the hepatitis C virus (HCV) RNA-dependent RNA polymerase, ...RNA replication, implicating it as an essential cis-acting replication ... See full document

... are required for viral infectivity (72) and viral replication in host cells (25, 42, ...the c-myc gene by binding to the FUSE. It also binds the 3 ⬘NTR of the GAP-43 mRNA and ... See full document

... was required to determine the contribution of individual nucleotides to the predicted RNA-RNA interactions with the upstream ..., C 9113 490 and U 9299 were not detrimental to replicon activity, whether ... See full document

... still replication competent, the distribution of substitu- tions within the replaced sequence meant that further site- directed mutagenesis was required to determine the contribu- tion of individual ... See full document

... (NTPi) required for RNA synthesis is typically greater than the concentration of NTPs required for ...small viral protein, is covalently attached to the 5 ⴕ end of picornavirus negative- and ... See full document

... RNA element plays a very important role in robust repli- cation in ...reclone viral genomes from mice inoculated with mut 5 or the wt were not successful due to a very limited amount of ...not ... See full document

... a cis-acting element required for efficient accumulation of monomer-length replicative-form ...a cis-acting element that inhibited replication of the ... See full document

... is required within this loop for efficient replication of subgenomic HRV-14 replicon ...RNA replication and their ability to function as template in an in vitro VPg uridylylation reaction, ... See full document

... their replication in ...for replication relative to the parental SIN 5 ⴕ ...pseudorevertant viral populations in BHK cells under competitive conditions yielded evolved, more homogeneous 5 ⴕ -terminal ... See full document

... structure required for cap-independent viral protein translation ...or viral genome ...in viral genome replication ...these cis elements in domain 1 (CΔD1-D2) severely diminished ... See full document

... RNA replication was due to reduced translation, increasing translation efficiency should enhance ...a cis-acting replication signal or inhibits RNA synthesis directly, increasing the level of ... See full document

... functional replication components present in HCV-infected ...HCV replication machinery in replicon cell, resulting in marked che- mosensitization of replicon cells to the cytotoxic effects of 5-FC, while ... See full document

... These enzymes would unambiguously distinguish between dif- ferent replicative intermediates as well as input plasmid DNA. DpnI selectively cleaves methylated input plasmid but is inac- tive against templates that have ... See full document

... The primers used are shown in Fig. 1 and were designed to link sequences in the C protein directly to the start of the signal sequence for NS1. Thus, the entire sequence encoding prM and E, as well as various ... See full document

... NS5A has an N-terminal AH that is necessary and sufficient for membrane localization and whose genetic disruption im- pairs HCV replication (5, 10). Our yeast two-hybrid results showed that the TBC1D20-NS5A ... See full document

... wild type animals. There was also a decrease in plasma phospholipids (75%) and triglycerides (45–58%) levels as compared to wild type animals [98]. SRBI and its ligand HDL are of significance in the early steps of HCV ... See full document

... infectious virus in the supernatant of transfected cells were then determined on cells expressing no, wild-type, or p3-4 ...miRNA-independent replication can occur ...wild-type virus made in cells ... See full document

... Thus, whereas passage of CD-61CATPmaCI resulted in in- creasing amounts of CAT, the amount of CAT protein pro- duced by CD-84CATXcmI-struct declined after P 0 . FIG. 4. D-RNAs made to investigate sequences necessary for ... See full document

... Although the integrity of stem 3 is clearly important for 2C loop function, other domains of the structure may also con- tribute. McKnight and Lemon showed that extensive mutagen- esis of the terminal loop of HRV14 CRE ... See full document