[PDF] Top 20 A cell-free replication system for human polyomavirus JC DNA.
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A cell-free replication system for human polyomavirus JC DNA.
... other replication proteins are sup- plied by the host (8, 23, 30, ...vitro replication assays for SV40 DNA has allowed the identification and characterization of the cellular proteins that are ... See full document
8
Inhibition of human diffuse large B cell lymphoma growth by JC polyomavirus like particles delivering a suicide gene
... expression system on a large scale at low cost [25], and the in vivo DNA packaging method developed for them [29], which replaces in vitro osmotic shock [74], not only increases their packaging efficiency ... See full document
9
Restriction of Human Polyomavirus BK Virus DNA Replication in Murine Cells and Extracts
... of polyomavirus-associated nephropathy (PVAN) and other ...BKV replication in the human kidney and the accompanying ...BKV replication in murine cells and extracts and the cause(s) of this ... See full document
10
The Retinoblastoma Protein Alters the Phosphorylation State of Polyomavirus Large T Antigen in Murine Cell Extracts and Inhibits Polyomavirus Origin DNA Replication
... pRb and Py T Ag was responsible for the observed inhibition. To address that question, we generated a recombinant bacu- lovirus that expresses a mutant Py T Ag, dl141, which lacks the pRb interaction motif, DLXCXE (41). ... See full document
10
Regulation of Human Polyomavirus JC Virus Gene Transcription by AP-1 in Glial Cells
... primary human fetal glial cell line that is transformed with replication-defective SV40 and serves as a convenient culture system for JCV infection (21), was infected with the hybrid JCV ... See full document
8
Human Papillomavirus DNA Replication Compartments in a Transient DNA Replication System
... small DNA viruses with circular, dou- ble-stranded genomes ...vegetative replication and the production of prog- eny virions are confined to the differentiated squamous cells in the upper strata (for a ... See full document
9
The DNA damage response promotes polyomavirus JC infection by nucleus to cytoplasm NF kappaB activation
... viral replication origin, induced DDR markers including γ - H2AX, p53 nuclear foci and other signaling components downstream of ATM and ATR kinases such as CHK1 and ...apparent DNA damage as measured by ... See full document
10
Replication of JC Virus DNA in the G144 Oligodendrocyte Cell Line Is Dependent Upon Akt
... JCV replication assay is that T-ag is expressed from a separate plasmid than the origin- containing Fluc plasmid; as a result, the regulation of T-ag transcription and viral replication are uncoupled (42, ... See full document
16
Host DNA Damage Response Factors Localize to Merkel Cell Polyomavirus DNA Replication Sites To Support Efficient Viral DNA Replication
... Merkel cell polyomavirus (MCPyV) in the development of Merkel cell carcinoma (MCC), making MCPyV the first polyomavirus to be clearly associated with human ...host DNA damage ... See full document
13
Age-Specific Seroprevalence of Merkel Cell Polyomavirus, BK Virus, and JC Virus
... Merkel cell polyomavirus (MCPyV), a new human polyoma- virus, was recently discovered by molecular techniques in Merkel cell carcinoma (MCC) (11), a rare and aggressive skin tumor (20, ... See full document
7
Comparison of real time PCR and hemagglutination assay for quantitation of human polyomavirus JC
... blood cell (RBC) agglutination and traditionally, hemag- glutination (HA) and HA inhibition (HAI) assays have been employed for quantitation of JCV ...of JC viral load by HA is often ...viral DNA ... See full document
5
Review on the role of the human Polyomavirus JC in the development of tumors
... of cell by JCPyV requires the binding be- tween the viral VP1 and an N-linked glycoprotein with sialic acid: JCPyV uses both the α(2,3)- and α(2,6)-linked sialic acids to infect the permissive glial cells ...host ... See full document
14
AP1 enhances polyomavirus DNA replication by promoting T-antigen-mediated unwinding of DNA.
... the replication complexes of polyomavirus and simian virus 40 has provided the means to determine how the replication of these viruses is ...of replication rely upon transcriptional activators ... See full document
5
Two defective DNAs of human polyomavirus JC adapted to growth in human embryonic kidney cells.
... Lanes: 1, pBR322 digested with BamHI plus EcoRI plus HindIII; 2, pJC1-4 digested with BamHI plus EcoRI plus HindIII; 3, pJC010 digested with BamHI plus EcoRI plus HindlIl; 4, pJC2-7 dige[r] ... See full document
7
Identification of critical elements within the JC virus DNA replication origin.
... The only origin plasmid that contained SV40 late sequences and exhibited detectable replication activity in POJ-19 cells was pJLo, a construct in which all but 22 nucleotides of the SV40[r] ... See full document
11
Nucleotide sequence of the region encompassing the JC virus origin of DNA replication.
... Similarities include palindromic and symmetrical sequences near their origins of DNA replication, binding sites for their large T proteins, an AT-rich region the Goldberg-Hogness, or TAT[r] ... See full document
7
Genetic analysis of the enhancer requirements for polyomavirus DNA replication in mice.
... Analysis of Py variants selected for replication in undifferentiated embryonal carcinoma cell lines PyF441, PyFlll showed that there was little change in levels of viral DNA replication [r] ... See full document
10
Mouse DNA primase plays the principal role in determination of permissiveness for polyomavirus DNA replication.
... The replication activities of PyV origin-containing DNA and SV40 origin-containing DNA were measured in the presence of various amounts of extracts prepared from the cells of various spe[r] ... See full document
8
Polyomavirus origin for DNA replication comprises multiple genetic elements.
... To more precisely delineate the boundaries of the polyomavirus ori we constructed a series of mutants that contain progressive deletions within viral DNA sequences originating from eithe[r] ... See full document
14
ERK Is a Critical Regulator of JC Polyomavirus Infection
... FIG 3 JCPyV induces multiphasic ERK activation upon infection. (A) SVG-A cells were infected with JCPyV (MOI ⫽ 1 FFU/cell) for the specified duration and were analyzed via Western blotting using antibodies specific ... See full document
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