[PDF] Top 20 Characterization of IS1245 for Strain Typing of Mycobacterium avium
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Characterization of IS1245 for Strain Typing of Mycobacterium avium
... that IS1245 (or homologous sequences) are widely prevalent among clini- cal isolates of ...M. avium and that Southern blot analysis of this element is useful for strain ...of IS1245 elements ... See full document
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Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR Restriction Enzyme Analysis
... M. avium in 111 swine isolates and 26 clinical MAC isolates by ...M. avium isolates were identified by PCR with the DT1-DT6 and IS1245 primers, nucleic acid hybridiza- tion with the AccuProbe system, ... See full document
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Rapid Detection and Typing of Strains of Mycobacterium avium subsp paratuberculosis from Broth Cultures
... dered due to its long incubation period, presence of undetec- ted subclinical cases, absence of rapid M. avium subsp. para- tuberculosis-specific diagnostic tools and efficacious vaccines, and lack of knowledge of ... See full document
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Molecular characterization of Mycobacterium avium complex isolates giving discordant results in AccuProbe tests by PCR restriction enzyme analysis, 16S rRNA gene sequencing, and DT1 DT6 PCR
... Based on cultural and biochemical tests, a total of 84 strains (72 clinical and 12 environmental isolates from the Caribbean Isles, Europe, and the Indian subcontinent) were identified as members of the ... See full document
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Comparison of a Variable Number Tandem Repeat (VNTR) Method for Typing Mycobacterium avium with Mycobacterial Interspersed Repetitive Unit VNTR and IS1245 Restriction Fragment Length Polymorphism Typing
... The primers used for MATR-VNTR are listed in Table 1. The PCR mix- ture (50 l) was composed of 2 l of DNA solution, 1 U of AmpliTaq Gold DNA polymerase (Applied Biosystems, Foster City, CA), 5 l of 2 mM ... See full document
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Comparison of Variable-Number Tandem-Repeat Markers typing and IS1245 Restriction Fragment Length Polymorphism fingerprinting of Mycobacterium avium subsp. hominissuis from human and porcine origins
... M. avium more often than any other NTM species ...M. avium from the mesenteric lymph nodes in of 48 out of 345 ...M. avium isolates can be relatively resistant to heating and therefore sur- vive in ... See full document
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Use of Multilocus Variable Number Tandem Repeat Analysis for Typing Mycobacterium avium subsp paratuberculosis
... RFLP typing of ...M. avium strain 104 which has a direct repeat region containing a 27-bp repeat and 12 ...M. avium strains with a DNA probe covering a large part of the direct repeat region ... See full document
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Mycobacterium Bovis Characterization through RD4 Deletion and Spoligotyping Techniques
... Region of Difference (RD)-4 Deletion Typing: For the RD4 deletion typing, the procedure described by Cadmus was used. Each sample was tested in a separate PCR tube. Primers directed against the RD4 were ... See full document
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Characterization of a Mycobacterium intracellulare Variant Strain by Molecular Techniques
... M. avium, even though they showed differences in the sequence of the 16S rDNA hypervariable region when com- pared to specific sequence of the ...type strain (Fig. 1A). They can be differentiated from M. ... See full document
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MOLECULAR CHARACTERIZATION OF MYCOBACTERIUM AVIUM SUBSP PARATUBERCULOSIS ISOLATES
... molecular characterization of prevalent MAP isolates from cattle, sheep, and goat demonstrated that IS1311 PCR with REA, which is based on point mutations in the IS1311, identified all 24 MAP isolates as ... See full document
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Genomic Characterization of the Vaccinal Strain of Mycobacterium Avium Subspecies Paratuberculosis (MAP) 316F by MIRU-VNTR
... of Mycobacterium avium complex in France (available at: ...this strain was resupplied to Razi by the Turkish collaborating center, ...this strain have been analyzed by MIRU-VNTR ... See full document
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IS1311 and IS1245 Restriction Fragment Length Polymorphism Analyses, Serotypes, and Drug Susceptibilities of Mycobacterium avium Complex Isolates Obtained from a Human Immunodeficiency Virus Negative Patient
... serological typing, probes (Accu-Probes, San Diego, ...M. avium complex isolates (27, 31, 36, 39, 41, 42, 45, ...M. avium complex yield positive reactions with the Accu-Probe M. avium complex ... See full document
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Prevalence of Mycobacterium avium in Slaughter Pigs in The Netherlands and Comparison of IS1245Restriction Fragment Length Polymorphism Patterns of Porcine and Human Isolates
... contained IS1245, which is characteristic of M. avium (2, 6, ...30 IS1245-containing MAC isolates were subjected to the Accuprobe test specific for ...M. avium, and they were found to be ... See full document
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Use of different molecular typing techniques for bacteriological follow up in a clinical trial with AIDS patients with Mycobacterium avium bacteremia
... M. avium culture was between 2 and 15 months (median, ...RFLP typing showed identical patterns for strains isolated throughout bacteriological fol- low-up for 37 patients, indicating monoclonal ...different ... See full document
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New Variable Number Tandem Repeat Markers for Typing Mycobacterium avium subsp paratuberculosis and M avium Strains: Comparison with IS900 and IS1245 Restriction Fragment Length Polymorphism Typing
... PCR-based typing markers consisting of variable-number tandem repeats (VNTRs) of genetic elements called mycobacterial interspersed repetitive units ...M. avium from ...MIRU-VNTR typing, with a ... See full document
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Typing of Clinical Mycobacterium avium Complex Strains Cultured during a 2 Year Period in Denmark by Using IS1245
... M. avium infection, since they were smear positive or had at least three specimens that were positive by culture, did not exhibit the most frequent patterns more often than isolates from patients with only one or ... See full document
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Identification of Mycobacterium avium Genotypes with Distinctive Traits by Combination of IS1245 Based Restriction Fragment Length Polymorphism and Restriction Analysis of hsp65
... reference strain, an isolate from a bird in a zoo (a crane [Grus virgo Linnaeus 1758]), and a pig ...M. avium (9, 14). Komijn et al. (9) studied 47 M. avium isolates from birds and showed that they ... See full document
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Loop Mediated Isothermal Amplification for Direct Detection of Mycobacterium tuberculosis Complex, M avium, and M intracellulare in Sputum Samples
... of Mycobacterium tuberculosis complex, Mycobacterium avium, and Mycobacterium intracellulare directly from sputum specimens as well as for detection of culture isolates grown in a liquid ... See full document
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Absence of Mycobacterium intracellulare and Presence of Mycobacterium chimaera in Household Water and Biofilm Samples of Patients in the United States with Mycobacterium avium Complex Respiratory Disease
... M. avium was the predominant cause of MAC lung disease (4). M. avium has also been shown to be the predominant or exclusive MAC species in water samples of residential ...M. avium, and only one ... See full document
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Clinical Analysis of Pulmonary Nontuberculous Mycobacterial Disease Diagnosed as Coincidental Pulmonary Infection Due to Mycobacterium Species*
... of Mycobacterium avium + Mycobacterium intracellulare in two patients, Mycobacterium avium + Mycobacterium kansasii in one, Mycobacterium intracellulare + ... See full document
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