[PDF] Top 20 Characterization of the Block in Replication of Nucleocapsid Protein Zinc Finger Mutants from Moloney Murine Leukemia Virus

... mutant virus-infected cells. The CCCC and CCHH mutants produced these DNA copies at greatly reduced ...amplified from two-long-terminal-repeat viral DNA (vDNA) by PCR, were cloned and ...isolated ... See full document

... ape leukemia virus-pseudotyped Moloney murine leukemia virus ...recovered from human fibrosarcoma HT1080 cells result in 10-fold-higher transduction rates ...recovered ... See full document

... retroviral nucleocapsid (NC) protein facilitates both DNA synthesis by reverse transcriptase (RT) and annealing of the nascent DNA with acceptor ...the murine leukemia virus (MLV) NC ... See full document

... retroviral nucleocapsid protein (NC) originates by cleavage of the Gag ...two zinc fingers. Mutations in either the basic residues or the zinc fingers can affect several events of the ... See full document

... 21 finger was made by introducing a C39H point mutation in the gene coding for the NC ...sequence from the PvuI site through nt ...21 finger. 4658-015 contains sequences from nt 2535 through ... See full document

... of replication, it has been difficult to study the effects of NC mutations on the early events of replication in ...made from RNA packaged by this ...this virus (64). Alteration of the MoMuLV ... See full document

... NCp10 zinc finger drastically impair infectiv- ity of MuLV and MuLV-VL30 recombinant ...of virus replica- tion, substitutions and deletions were introduced into Friend MuLV NCp10 coding sequence in ... See full document

... Cell lines producing the mutant virions were constructed in NIH 3T3 and rat2 cells, and the viral particles released by these cells were characterized for protein and RNA content.. The r[r] ... See full document

... To investigate the role of the basic amino acids flanking the NCplO zinc finger, neutral residues were substituted for the basic amino acids and the effects of these mutations in vivo on[r] ... See full document

... immunodeficiency virus type 1 (HIV-1) particles and oxidize the cysteine thiolates in the NC zinc ...on Moloney murine leukemia virus ...foamy virus, a spumaretrovirus ... See full document

... in Moloney murine leukemia virus (MoMLV) replication, we analyzed 18 insertional mutations in this ...All mutants were ...loop. Mutants of this region assembled imperfect ... See full document

... In this study, saturation experiments required: i preparation of large quantities of DNA complementary to the genome of M-MuLV cDNA by the endogenous reverse transcriptase reaction, and [r] ... See full document

... On the other hand, earlier data on the complexity of the RNA of M-MLV obtained by hybridizing viral RNA with complementary DNA and 9 Downloaded from http://jvi.asm.org/ on November 10, 2[r] ... See full document

... PG13 cell clones containing MSTK vector proviruses were established by infection with virus produced in GP+E-86 helper cells at low multiplicity of infection and used in the forward muta[r] ... See full document

... tested replication properties for Moloney murine leukemia viruses with targeted mutations in the PPT and in conserved sequences upstream, as well as for pools of mutants with randomized ... See full document

... envelope protein to mediate cell-cell fusion were also detrimental to retroviral ...the mutants that allowed extensive cell-cell fusion producing virions with very low ...infectious virus were a ... See full document

... Murine leukemia virus pol gene products: analysis with antisera generated against reverse transcriptase and endonuclease fusion proteins expressed in Escherichia coli.. Affinity purifica[r] ... See full document

... Construction and analysis of deletion mutations in the pol gene of Moloney murine leukemia virus: a new viral function required for productive infection.. Highly preferred targets for re[r] ... See full document

... Identification of the bases in the RNase T2-resistant oligonucleotide fraction and base-methylated nucleotides derived from the interior of the subunit molecules involved the following c[r] ... See full document

... Gag protein, glycosylated Gag (glyco-gag). In Moloney murine leukemia virus (M-MuLV) glyco-gag (gPr80 gag ) is translated from unspliced viral mRNA via an upstream CUG initiation ... See full document