[PDF] Top 20 Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus
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Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus
... Rational clinical application of quantitative assessments of hepatitis C virus (HCV) RNA depends on an understanding of factors affecting the assay and its intrinsic ...available ... See full document
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Assessment of the Target Capture PCR Hepatitis B Virus (HBV) DNA Quantitative Assay and Comparison with Commercial HBV DNA Quantitative Assays
... Each of the tests for HBV has been developed by using proprietary HBV DNA standards and provides results in units of measure unique to that particular method. The inconsis- tency among various units of measure for HBV ... See full document
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Evaluation of a quantitative competitive PCR assay for measuring herpes simplex virus DNA content in genital tract secretions
... previous semiquantitative detection method and found concordance for 61 of 63 positive specimens. We also evaluated the HSV DNA content from individual swabs of genital secretions obtained from individual sites of the ... See full document
5
Quantitative Competitive Reverse Transcription PCR for Quantification of Dengue Virus RNA
... QC-RT- PCR has been successfully utilized for other viruses, such as human immunodeficiency virus type 1 and HCV, and this method has been shown to be useful for assessing both clinical status and ... See full document
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Anti hepatitis B core antigen testing with detection and characterization of occult hepatitis B virus by an in house nucleic acid testing among blood donors in Behrampur, Ganjam, Orissa in southeastern India: implications for transfusion
... An in house nucleic acid tests (NAT) to directly detect the presence of HBV DNA using a combination of amplification and detection techniques similar to HCV RNA was employed [22]. HBV DNA isolation and amplification by ... See full document
7
Comparison of Quantitative Competitive PCR with LightCycler Based PCR for Measuring Epstein Barr Virus DNA Load in Clinical Specimens
... real-time PCR assay for EBV load ...trarapid PCR system based on real-time fluorimetric quantifi- cation of PCR ...of PCR products in this assays ensures high ...LightCycler ... See full document
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Simultaneous Genotyping and Quantification of Hepatitis B Virus for Genotypes B and C by Real Time PCR Assay
... commercial assay with good statistical ...GQ-PCR assay and that obtained by direct sequencing (Table ...127 clinical samples, we found that 17 ...type-specific PCR assay. Because ... See full document
8
Novel, ligation dependent PCR assay for detection of hepatitis C in serum
... of PCR in clin- ical ...from clinical specimens involves laborious and time- consuming procedures that are impractical for processing large numbers of specimens in a clinical laboratory ...which ... See full document
7
Development of a Real Time Quantitative PCR for Detecting Duck Hepatitis A Virus Genotype C
... duck hepatitis A virus genotype C (DHAV-C), a causative agent of duck viral hepatitis, has been responsible for in- creasing economic losses in the duck industry in China and South ... See full document
6
TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... one assay that also used in vitro-transcribed RNA ...the quantitative real-time RT-PCR pre- sented ...quantify virus in the samples tested in this study by use of a standard curve generated ... See full document
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Clinical Performance Evaluation of Four Automated Chemiluminescence Immunoassays for Hepatitis C Virus Antibody Detection
... the clinical specificity of four CLIAs. The clinical specificity was ...Architect assay, ...the assay format may be responsible for nonspecific reactivity (11, ...the assay format seems ... See full document
5
Hepatitis C Virus Subtyping by a Core Envelope 1 Based Reverse Transcriptase PCR Assay with Sequencing and Its Use in Determining Subtype Distribution among Danish Patients
... transcriptase PCR (RT-PCR) assay using conserved primers deduced from the core-envelope 1 (C-E1) region of the hepatitis C virus (HCV) genome was developed for subtyping ... See full document
10
Comparison of a competitive combined reverse transcription PCR assay with a branched DNA assay for hepatitis C virus RNA quantitation
... compared quantitative RT-PCR and bDNA assays, obtaining correlation coefficients similar to that obtained by us (2, ...the assay to a large number of specimens. Results of the RT-PCR ... See full document
5
Quantitation of hepatitis C virus genome molecules in plasma samples
... A competitive reverse transcription PCR cRT-PCR-based assay for the quantitative detection of hepatitis C virus HCV viremia was developed, optimized, and applied to the direct molecular [r] ... See full document
6
Development of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA
... the PCR. Regarding sensitivity, our PCR assay could detect about 10 copies, and measured DNA quantitatively from 10 to 100,000 copies in the extracts ...to PCR reaction did not cause a ... See full document
5
Evaluation of the Abbott LCx HIV 1 RNA Quantitative, a New Assay for Quantitative Determination of Human Immunodeficiency Virus Type 1 RNA
... the assay controls and clinical samples were ex- tracted by centrifugation and separation using a QIAAmp col- umn and subjected to amplification with primers targeted at a highly conserved 170-bp sequence ... See full document
5
Hepatitis C Virus (HCV) Core Antigen Assay To Detect Ongoing HCV Infection in Thai Injection Drug Users
... a quantitative enzyme immunoassay (trak-C) for hepatitis C virus core antigen (HCV core Ag) by testing serum specimens from 820 injection drug users in Thailand with anti-HCV ... See full document
6
Molecular Detection and Species Specific Identification of Medically Important Aspergillus Species by Real Time PCR in Experimental Invasive Pulmonary Aspergillosis
... real-time PCR system demonstrated greater sensitivity than culture for detection of ...Pan-Aspergillus PCR was also more sensitive than culture for detection of ...real-time PCR and galactomannan ... See full document
8
Characterization of hepatitis C virus resistance to grazoprevir reveals complex patterns of mutations following on-treatment breakthrough that are not observed at relapse
... the virus while on treatment after previously having undetectable HCV ...vitro. Virus from this partici- pant underwent selection of an A156L variant that involved 2 nucleotide changes (GCC > CTC), with ... See full document
17
Epidemiology of Hepatitis B Virus Genetic Diversity Vs and Diagnostic Molecular Markers of Hepatitis C Virus Core Gene
... the virus cause diagnostic markers including hepatitis B virus genotypes Vs and seeking nuclear gene has been mutated ...the virus in different countries and the relationship between genotype ... See full document
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