[PDF] Top 20 Clinical Evaluation of Two Methods for Genotyping Hepatitis C Virus Based on Analysis of the 5′ Noncoding Region
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Clinical Evaluation of Two Methods for Genotyping Hepatitis C Virus Based on Analysis of the 5′ Noncoding Region
... four clinical specimens that potentially contained mixtures of genotypes 1a and ...4, 5, and ...their analysis of 448 se- quences present in several large databases. Probes 5 and 6 are used to ... See full document
7
Comparison of Hepatitis C Virus Genotyping by 5′ Noncoding Region and Core Based Reverse Transcriptase PCR Assay with Sequencing and Use of the Assay for Determining Subtype Distribution in India
... Phylogenetic analysis of the sequences of the 5 ⴕ noncoding regions (5 ⴕ NCR) of 149 samples from hepatitis C virus (HCV) RNA-positive chronic carriers representing ... See full document
5
Evaluation of a New Assay in Comparison with Reverse Hybridization and Sequencing Methods for Hepatitis C Virus Genotyping Targeting Both 5′ Noncoding and Nonstructural 5b Genomic Regions
... the evaluation of a new real-time PCR assay for hepatitis C virus (HCV) ...the 5 ⴕ noncoding region (5 ⴕ NC) (method 1; HCV genotyping analyte-specific ... See full document
6
Concordance of hepatitis C virus typing methods based on restriction fragment length polymorphism analysis in 5' noncoding region and NS4 serotyping, but not in core PCR or a line probe assay
... the 5 9 NC and core regions of HCV (nucleotides 279 to 512) were ...to 5% of the 5 9 NC sequences of HCV genotype 1 may have a mutation in nucle- otide 99 which could induce a false 1a-1b ...the ... See full document
5
Review: Molecular Diagnosis of Hepatitis C Viruses; Technologies and Its Clinical Applications
... have two to three fold higher response rate than the patients infected with genotype 1 ...HCV genotyping assay for treatment management and ultimate patient care is ...sequencing analysis of specific ... See full document
33
Comparison of Hepatitis C Virus NS5b and 5′ Noncoding Gene Sequencing Methods in a Multicenter Study
... NS5b-based genotyping procedures are preferable to 5 ⬘ NC- region-based ones (6, 26, ...than 5 ⬘ NC-region-based meth- ods, with the genotypes in only 2 samples ... See full document
7
Evaluation of the Invader Assay for Genotyping Hepatitis C Virus
... NS5B Genotyping Assay was attempted with this specimen but failed to generate a ...HCV 5 ⬘ NC region sequence of this clone was consistent with that of genotype 3b, further examination of the ... See full document
6
Rapid Genotyping of Hepatitis C Virus by Primer Specific Extension Analysis
... accurate genotyping of hepatitis C virus (HCV) is becoming increasingly important for clinical management of chronic infection and as an epidemiological ...has clinical ... See full document
6
Analysis of the 5′ Noncoding Region versus the NS5b Region in Genotyping Hepatitis C Virus Isolates from Blood Donors in France
... studies based on 5 ⬘ NCR analysis which established that subtype 1b was more prevalent than subtype 1a (range of 27 to 34% versus range of 16% to 20%, respectively) (7, 18, ...that 5 ⬘ NCR ... See full document
6
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
... Hepatitis C virus genotyping assays are usually based on the sequence analysis of an amplified segment of the genome, which is commonly the 5’ untranslated ...Currently, ... See full document
5
Hepatitis C Virus Genotyping: Interrogation of the 5′ Untranslated Region Cannot Accurately Distinguish Genotypes 1a and 1b
... identified clinical differences only between those infected with type 1 isolates and those infected with non-type 1 ...investigating clinical differences between patients in- fected with HCV genotypes 1a ... See full document
8
Simplified Hepatitis C Virus Genotyping by Heteroduplex Mobility Analysis
... for genotyping HCV is nucleotide sequencing of NS5b ampli- ...routine genotyping. Many methods have been published, although no simple, in- expensive, and accurate method has yet been developed ... See full document
6
Hepatitis C Virus Genotyping Using an Oligonucleotide Microarray Based on the NS5B Sequence
... Several methods has been proposed for HCV genotyping (50), including commercially available techniques based on real-time PCR: the HCV genotyping analyte-specific reagent (ASR) assay (Abbott ... See full document
8
Nucleotide Variability and Translation Efficiency of the 5′ Untranslated Region of Hepatitis A Virus: Update from Clinical Isolates Associated with Mild and Severe Hepatitis
... fulminant hepatitis A, several studies have searched for viral determi- nants of disease severity by comparing the sequences of field isolates associated with different clinical presentations (13, 26), and ... See full document
9
The Image of the Region Element Based on the Activities of Pedestrians
... The results of the mapping behavior of Tugu area can be seen in figure 4 and figure 5. The results show that the type of primary activity on both weekday and weekend is similar, but there are more primary activity ... See full document
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Cytoplasmic Expression of mRNAs Containing the Internal Ribosome Entry Site and 3′ Noncoding Region of Hepatitis C Virus: Effects of the 3′ Leader on mRNA Translation and mRNA Stability
... coding region surrounded by specific NCR ...predicted 5⬘ and 3⬘ NCR ...example, 5⬘HCVluc RNAs might be more abundant than 5⬘HCVluc3⬘HCV RNAs but may be less efficiently ... See full document
6
Economic evaluation of screening programs for hepatitis C virus infection: evidence from literature
... Our review exhibits two main limitations. Firstly, the inclusion criteria for study selection did not consider the methodological quality or the quality of reporting of the records identified. On the other hand, ... See full document
10
Hepatitis C virus detection by single round PCR specific for the terminal 3' noncoding region
... nested 5 9 NCR PCR results are unclear, but it is possible that the nested 5 9 NCR PCR assay has a slight advantage in ...some 5 9 NCR-based RT-PCR assays (17, 22, 33) is the small amplicon ... See full document
7
Simeprevir for the treatment of hepatitis C virus infection
... are accompanied by chronic liver disease, leading to fibrosis and cirrhosis, with a risk of complications including ascites and hepatocellular carcinoma. Over recent decades, the standard treatment for HCV genotype 1 ... See full document
9
Sequences in the 5′ Nontranslated Region of Hepatitis C Virus Required for RNA Replication
... HCV 5⬘ NTR were fused via the 63-nucleotide spacer to the IRES of PV were used to program HeLa cell extracts as described in Materials and ...HCV 5⬘ NTR is shown in lane 3 (rep5.1). Note that this RNA ... See full document
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