[PDF] Top 20 Cloning and Expression of a Helicobacter bilis Immunoreactive Protein
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Cloning and Expression of a Helicobacter bilis Immunoreactive Protein
... different immunoreactive clones, 13 were determined to contain either P167 or P158: 5 clones contained the complete sequence of P167; 6 clones contained partial sequences of P167; and 2 clones contained the ... See full document
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IN SILICO DESIGN OF ANTIGENIC PEPTIDES AND MOLECULAR CLONING OF CYTOTOXIN ASSOCIATED GENE (cagA) OF H PYLORI IN AN EXPRESSION VECTOR
... Gene Expression Studies: The purified gene of the insert was then used for expression ...pMD19-T cloning vector (Fermentas, ...the cloning as like in 1:1 vector to insert ratio was ... See full document
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Cloning and Expression of Coxsakievirus B3 Viral Protein-1 in E. Coli
... Viral protein-1 (VP1) is a major capsid protein of Coxsakievirus B3 (CVB3) that plays an important role in directing viruses towards permissive cells and acts as a main antigenic site of the virus in ... See full document
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CLONING AND EXPRESSION OF A PARTIAL UREA ANTIGEN FOR THE PRODUCTION OF VACCINE AGAINST HELICOBACTER PYLORI, THE RISK FACTOR FOR GASTRIC CANCER
... the cloning vector was ligated to expression ...the expression of ureA protein and the protein was successfully isolated and ...quantified protein was subjected to SDS PAGE to ... See full document
7
Cloning and developmental expression of zebrafish pdzrn3
... the cloning of pdzrn3 and show that this gene displays a dynamic expression during zebrafish ...spatio-temporal expression pattern during embryogenesis was not previously ...of expression of ... See full document
5
Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein
... regarding cloning, expression and immuno-reactivity characterization of recombi- nant GRA5 protein of ...GRA5 protein cloned and expressed for detection of human toxoplasmosis us- ing western ... See full document
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Genesis of newt sperm axial fiber: cDNA cloning and expression of a 29 kDa protein, a major component of the axial fiber, during spermatogenesis
... Int J IJC\ 8iul ~O 1109 1118(19961 1109 Origillal,111hk Genesis of newt sperm axial fiber cDNA cloning and expression of a 29 kDa protein, a major component of the axial fiber, during spermatogenesis[.] ... See full document
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Cloning and expression of class I chitinases in Hami melon after Penicillium infection.
... HmCHT-2-encoded protein was ...this protein was predicted to be 34.13, which indicated that this protein was ...the protein is ...HmCHT-2-encoded protein and that the entire peptide ... See full document
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Cloning and Expression of Recombinant Helicobacter pylori Urease A and B Subunits as a Putative Vaccine
... associated lymphoid tissue (MALT) lymphoma [7]. Urease is an important colonization factor of H pylori such that urease negative mutants are unable to colonize the stomachs of mice [8]. Urease activity is required for ... See full document
5
Cloning, Expression, and Immunological Characterization of the P30 Protein of Mycoplasma pneumoniae
... For expression, subcloning of the full-length P30 protein (P30A) and a frag- ment of the p30 gene (P30B) was done in the pQE-30 vector and the pMAL-p2x fusion vector, according to the manufacturer’s ... See full document
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Cloning, expression and characterization of gE protein of Duck plague virus
... The purified denatured protein was done by gradient dialysis in 0.85% NaCl solution containing 6, 5, 4, 3, 2 M urea, and changed 3 times over 1 day at 4°C in each solu- tion. Also, aggregation was removed by ... See full document
11
Cloning and expression of an equine herpesvirus 1 origin-binding protein.
... In order to confirm that the C-terminal DNA-binding domain of EHV-1 OBP was mediating the same sequencespecific binding as the wild-type protein, in vitro-expressed del 1-499 was used to[r] ... See full document
8
Cloning and Expression of Immunoreactive Antigens from Mycobacterium tuberculosis
... After transfer, membranes were washed twice in TBST buffer and blocked in 5% skim milk-TBST. Pooled sera from four individuals with active TB and from four healthy individuals were preabsorbed overnight, against ... See full document
7
Cloning and Expression of an Immunogenic Membrane-Associated Protein of Helicobacter hepaticus for Use in an Enzyme-Linked Immunosorbent Assay
... 18-kDa immunoreactive protein, MAP18, was ...binant protein was expressed in Escherichia coli as a glutathione S-transferase (GST) fusion protein, GST- MAP18, and purified by affinity ... See full document
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Molecular Cloning of the Gene for a Conserved Major Immunoreactive 28-Kilodalton Protein of Ehrlichia canis: a Potential Serodiagnostic Antigen
... able expression of homologous p28 genes, thus enabling ...MSP-3 protein, resulting in persistent infections ...gene expression resulting in pheno- typic changes in Borrelia hermsii has also been ... See full document
8
RNA-seq analysis of Macrobrachium rosenbergii hepatopancreas in response to Vibrio parahaemolyticus infection
... A cutting edge technology has emerged recently, known as Next Generation Sequencing technology (NGS). Cur- rently, there are four established platforms which uses NGS technology: the Illumina Genome Analyzer, the ... See full document
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Synthesis and Production of Sweet-Tasting Protein in E. coli and Purification by Amylose Resin
... the expression vectors pMAL-C2X and pGEX-2T contain the strong Ptac ...the protein product that is required for folding and stability of the ...constructed expression vectors were transformed into ... See full document
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2. Tingting Tao, Xiaomeng Liu, Jie Chang and Feng Xu
... The 3-hydroxy-3-methylglutaryl-Coenzyme A reductase (HMGR), which catalyzes the conversion of HMG-CoA to MVA, is a rate-limiting enzyme in isoprenoid biosynthesis via MVA pathway (Rui et al., 2012; Dai et al., 2011). In ... See full document
8
Expression and Immunogenicity of Recombinant Immunoreactive Surface Protein 2 of Anaplasma phagocytophilum
... Cloning, expression, and purification of the recombinant MSP2 ...recombinant protein was performed by using Ni-NTA (nitrilotriacetic acid) His-bind resin as described by the manufacturer (Novagen, ... See full document
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<em>Helicobacter pylori</em> promotes gastric epithelial cell survival through the PLK1/PI3K/Akt pathway
... of immunoreactive cells from 100 cells in each field was averaged from the five fields and scored as follows: 0 ...5.0% immunoreactive; 1 = ...overall protein expression level was then ... See full document
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