[PDF] Top 20 Cloning and Expression of a Human T-Lymphotropic Virus Type 1 Protein with Reverse Transcriptase Activity
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Cloning and Expression of a Human T-Lymphotropic Virus Type 1 Protein with Reverse Transcriptase Activity
... for 1 min, 55°C for 1 min, and 72°C for ...Table 1. Primer MO40 anneals to nucleotides 2619 to 2632 of HTLV-1 (MT-2, accession ...the expression vector pGEX- 4T-1 ... See full document
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Role for Human Immunodeficiency Virus Type 1 Tat Protein in Suppression of Viral Reverse Transcriptase Activity during Late Stages of Viral Replication
... RT activity (Fig. 1, 2, and 4), and our in vivo results, showing both superior replication capacity and more-efficient endogenous RT activity in the molecular clones that encode 2-exon-Tat ...RT ... See full document
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Human T-Lymphotropic Virus Type 1 Mitochondrion-Localizing Protein p13II Sensitizes Jurkat T Cells to Ras-Mediated Apoptosis
... Jurkat T cells to FasL-induced apoptosis and to test the spec- ificity of FTI as an inhibitor of Ras function, we performed a transfection experiment where the Jurkat T cells were tran- siently transfected ... See full document
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Involvement of transcription factor YB-1 in human T-cell lymphotropic virus type I basal gene expression.
... The five-base-substitution mutant of oligonucleotide + 195 to +240 was previously shown to decrease binding of the Jurkat DRE 1-binding protein, p37 The human T-cell lymphotropic virus t[r] ... See full document
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Resistance to Nucleoside Analog Reverse Transcriptase Inhibitors Mediated by Human Immunodeficiency Virus Type 1 p6 Protein
... APP/SPT virus in the presence or absence of zido- vudine in comparison to the wild-type parental ...NL4-3 virus in the presence of subinhibitory concentrations of zidovudine ...in reverse ... See full document
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Construction and Characterization of a Temperature-Sensitive Human Immunodeficiency Virus Type 1 Reverse Transcriptase Mutant
... the human growth hormone, this mutagenesis approach resulted in the identifi- cation of residues involved in hormone binding ...only 1 of 33 HIV-1 RT mutants was ts, and a previous report describing ... See full document
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Human T-Lymphotropic Virus Type 1 Mitochondrion-Localizing Protein p13II Is Required for Viral Infectivity In Vivo
... for 1 min, annealing at 62°C for 1 min, and extension at 72°C for 45 s, followed by a final extension at 72°C for 5 ...; reverse, 5 ⬘ -GAG GCA GGG ATG ATG TTC-3 ⬘ ... See full document
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Activation of Nuclear Factor of Activated T Cells by Human T-Lymphotropic Virus Type 1 Accessory Protein p12I
... Jurkat T cells with a combination of anti-CD3 and CD28 antibodies resulted in significantly increased absolute values of NFAT- luciferase activity (377 ⫾ 36 [standard error of the mean (SEM)] untreated to ... See full document
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Human T-Lymphotropic Virus Type 1 p30IIRegulates Gene Transcription by Binding CREB Binding Protein/p300
... binding protein (CBP) and p300 mediate transcriptional control of various cellular and viral DNA binding transcription ...(HAT) activity, and form complexes with proteins such as CBP/p300 binding ... See full document
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Imbalanced expression of glutamate-glutamine cycle enzymes induced by human T-cell lymphotropic virus type 1 Tax protein in cultivated astrocytes.
... synaptic activity, since conversion of glutamate by the astrocyte is also seen along axonal pathways ...GS activity in human pathology is well ... See full document
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HuR interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells
... HuR expression with three different siRNAs targeting three different sites in the HuR mRNA sequence, we demonstrated that HuR expression was required for an optimal HIV-1 replication cycle and for ... See full document
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Immunogenicity of Mutations Induced by Nucleoside Reverse Transcriptase Inhibitors for Human Immunodeficiency Virus Type 1-Specific Cytotoxic T Cells
... CD8 T cells, except around mutation ...CD8 T cells that have been already primed and amplified in vivo and produce IFN-␥ ...cytolytic activity in Epstein-Barr virus infection ...CD8 T ... See full document
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Inhibitors of Human Immunodeficiency Virus Type 1 Reverse Transcriptase Target Distinct Phases of Early Reverse Transcription
... endogenous reverse transcription (ERT) assays (39, ...transmembrane protein, which render the HIV-1 envelope permeable to a range of small molecules ...microenvironment. Virus isolated from ... See full document
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Effects of Human Immunodeficiency Virus Type 1 Resistance to Protease Inhibitors on Reverse Transcriptase Processing, Activity, and Drug Sensitivity
... plasma virus were compared to iso- genic clones harboring the corresponding pretherapy protease ...and cloning of protease sequences were as previously described (20, ...each virus pair and analyzed ... See full document
5
Costimulation of cytokine gene expression in T cells by the human T leukemia/lymphotropic virus type 1 trans activator Tax.
... of T cells through their antigen-specific T-cell receptor results in the coordinate expression of a number of cytokine genes such as interleukin-2 (IL-2), IL-3, granulocyte- macrophage ... See full document
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Expression of human immunodeficiency virus type 1 reverse transcriptase in trans during virion release and after infection.
... cells, expression of the b -galactosidase gene is contingent on the expression of HIV-1 Tat trans-activator ...protein. Reverse transcription of the viral RNA into double-stranded viral ... See full document
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Neuropilin-1 Is Involved in Human T-Cell Lymphotropic Virus Type 1 Entry
... surface expression of endogenous NRP1 by HeLa-Tat and MDA-MB-453 ...-Galactosidase activity was determined after sub- tracting the background from a coculture of Cos-HIVLTRLacZ trans- fected with the ... See full document
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Differential Expression of Nur77 Family Members in Human T-Lymphotropic Virus Type 1-Infected Cells: Transactivation of theTR3/nur77 Gene by Tax Protein
... TR3/Nur77 expression can also be quickly induced by focal mechanical lesion of the brain, excitatory epileptogenic treatments with pilocarpine, and in vitro depolarization of neu- ral cells (17, ...infected ... See full document
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Inhibition of p53 Transactivation Function by the Human T-Cell Lymphotropic Virus Type 1 Tax Protein
... Jurkat T cells were ...Tax protein inhibited the ability of p53 to activate expression from the promoter in a dose-dependent fashion ...bars 1 to 3). A 33-fold reduction in p53-dependent Luc ... See full document
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Modulation of the Brd4/P-TEFb Interaction by the Human T-Lymphotropic Virus Type 1 Tax Protein
... Brd4 activity with P-TEFb have been ...P-TEFb activity through a novel auto- phosphorylation pathway that regulates CDK9 kinase activity on the Pol II ...the activity of the cellular Brd4 ... See full document
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