[PDF] Top 20 Comparison of Five Repetitive Sequence Based PCR Typing Methods for Molecular Discrimination of Salmonella enterica Isolates
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Comparison of Five Repetitive Sequence Based PCR Typing Methods for Molecular Discrimination of Salmonella enterica Isolates
... all isolates with the same serotype had the same ...the isolates with the same serotype still clustered together at a similarity coefficient of 85% or higher, except for two serotype Enteritidis ...lence ... See full document
9
Comparison of an Automated Repetitive Sequence Based PCR Microbial Typing System with Pulsed Field Gel Electrophoresis for Molecular Typing of Vancomycin Resistant Enterococcus faecium
... Repetitive-sequence-based PCR. The genetic relatedness of the VRE isolates was determined by Rep-PCR typing as previously described ...Rep- PCR was performed using ... See full document
5
Molecular Genotyping of Staphylococcus aureus Strains: Comparison of Repetitive Element Sequence Based PCR with Various Typing Methods and Isolation of a Novel Epidemicity Marker
... Rep-PCR typing appears to be superior to AP-PCR, since the rep-PCR results obtained in this study are highly repro- ducible due to the high annealing conditions of the ...rep-PCR ... See full document
8
Microbial DNA Typing by Automated Repetitive Sequence Based PCR
... Common molecular typing methods used for bacterial strain discrimination include PFGE, RAPD, AFLP, and ribotyping (20, 24, 27, 38, 40, 41, ...greater discrimination to evaluate results ... See full document
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Comparison of Different PCR Approaches for Typing of Francisella tularensis Strains
... the isolates. Therefore, there is a need to develop new typ- ing methods for this bacterial ...species. Repetitive element sequence-based PCR (rep-PCR) is a group of ... See full document
7
Molecular Typing of Klebsiella pneumoniae Isolates by Enterobacterial Repetitive Intergenic Consensus (ERIC)–PCR
... Thirty-five isolates were selected for analysis by ERIC-PCR based on clinical samples type (urine, trachea, wound, and blood), MDR isolates antibiotic resistance profile, sampling ... See full document
5
Comparison of Typing Methods with a New Procedure Based on Sequence Characterization for Salmonella Serovar Prediction
... the isolates representing the 40 most common serovars and of 86% of the isolates representing less-common ...probe-based molecular-serotyping approaches, we are not aware of any comprehensive ... See full document
12
CRISPR typing and subtyping for improved laboratory surveillance of salmonella infections
... of isolates to public health laboratories and PFGE results ...and comparison of banding profiles is not straightforward, even with standard protocols and analysis ...DNA sequence repeats in the ... See full document
21
Molecular Typing of Salmonella enterica Serovar Typhi Isolates from Various Countries in Asia by a Multiplex PCR Assay on Variable Number Tandem Repeats
... the isolates (Table 2 and ...multiplex PCR confirmed that the results were reproducible. Comparison of the VNTR profiles with the phage-typing data revealed that isolates with the same ... See full document
7
Evaluation of IS200 PCR and Comparison with Other Molecular Markers To Trace Salmonella enterica subsp enterica Serotype Typhimurium Bovine Isolates from Farm to Meat
... original molecular marker (IS200-PCR) and that is based on the amplification of DNA with outward-facing primers complementary to each end of IS200 has been evaluated with a collection of 85 ... See full document
6
Comparison of the DiversiLab Repetitive Element PCR System with spa Typing and Pulsed Field Gel Electrophoresis for Clonal Characterization of Methicillin Resistant Staphylococcus aureus
... ever, typing methods have also become an indispensable tool for distinguishing clonal nosocomial clusters from unrelated increase of MRSA ...genetic typing methods for the clonal ... See full document
7
Suitability of Repetitive DNA Sequence Based PCR Fingerprinting for Characterizing Epidemic Isolates of Salmonella enterica Serovar Saintpaul
... Three molecular typing methods, repetitive-sequence-based PCR (rep-PCR) fingerprinting, plasmid profil- ing, and arbitrarily primed PCR fingerprinting, were ... See full document
6
Efficacy of Pulsed Field Gel Electrophoresis and Repetitive Element Sequence Based PCR in Typing of Salmonella Isolates from Assam, India
... of discrimination observed in the present study, it may be concluded that PFGE is a better method for molecular typing of Salmonella than ...one molecular typing method and those ... See full document
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Epidemiology, Clinical Presentation, Laboratory Diagnosis, Antimicrobial Resistance, and Antimicrobial Management of Invasive Salmonella Infections
... of Salmonella serovars Typhi and Paratyphi A had emerged that were less susceptible to the ...520–522). Isolates that have this de- creased-susceptibility phenotype were not defined as resistant by the ... See full document
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<p>Molecular Relatedness of <em>Salmonella enterica</em> Typhimurium Isolates from Feces and an Infected Surgical Wound</p>
... SM080 isolates were genotyped using multi- locus sequence typing (MLST) and pulsed- fi eld gel electro- phoresis ...cular typing method of Salmonella serotype PFGE in ...PulsenetChina. ... See full document
6
Characterization of Salmonella enterica Serovar Typhimurium and Monophasic Salmonella Serovar 1,4,[5],12:i: Isolates in Thailand
... Duplex PCR was developed to screen Salmonella enterica serovar Typhimurium phage type DT104 and related strains in Thailand because a phage typing laboratory of serovar Typhimurium is not ... See full document
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Multilocus Sequence Typing Lacks the Discriminatory Ability of Pulsed Field Gel Electrophoresis for Typing Salmonella enterica Serovar Typhimurium
... Briefly, Salmonella isolates were grown overnight on MacConkey agar (Difco Becton Dickinson, MD) plates and then suspended in a cell suspension buffer (100 mM Tris–100 mM EDTA, pH ... See full document
5
Multiple Polymorphic Loci for Molecular Typing of Strains of Mycobacterium leprae
... leprae isolates from patients in Japan (except Okinawa) and Korea carried three copies of the repeat unit, while those from Brazil, Haiti, and Okinawa, Japan, had four ...clinical isolates are evaluated for ... See full document
7
Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus
... Phage typing, plasmid DNA restriction analysis, and antibiogram analysis, the techniques most readily available to clinical laboratories, identified 23 to 26 of 29 outbreak-related isola[r] ... See full document
9
Comparison of Multilocus Sequence Typing, Pulsed Field Gel Electrophoresis, and Antimicrobial Susceptibility Typing for Characterization of Salmonella enterica Serotype Newport Isolates
... of Salmonella enterica serotype Newport (commonly referred to as MDR-AmpC) have emerged in animals and humans and have become a major public health ...standard” typing method for Salmonella, ... See full document
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