[PDF] Top 20 Complement Activation Is Required for Induction of a Protective Antibody Response against West Nile Virus Infection

... the virus was abol- ished completely after exposure to a 10% (vol/vol) solution of ...fix complement (E8, IgG1) demonstrated no enhancement of complement-mediated neu- ...of complement, a ... See full document

... The complement system also acts to limit viral dissemination through antibody- independent ...distinct complement receptors (CR1, CR2, CR3, CR4, C1qR, CRIg, and SIGN-R1) have been identified as ... See full document

... IgG response were observed with wild-type and W101R E proteins, although a trend was observed at day 30 and after boosting (P ⫽ ...Epitope-specific antibody responses in human patient se- ...body ... See full document

... and protective efficacy of a recombinant subunit West Nile virus (WNV) vaccine was evaluated in rhesus macaques (Macaca ...monoclonal antibody specific for flavivirus E ...potent ... See full document

... antiviral response to WNV in C57BL/6 ...pathway(s) required for poly(I) · poly(C)-dependent induction of 2-5A Oas mRNA was intact in both C57BL/6 RNase L ⫹/⫹ and RNase L ⫺/⫺ mice ...gene ... See full document

... WNV infection; however, there were distinct differences in the ability of these antibodies to protect mice, both before and after ...of antibody is needed to effectively treat mice later in the course of ... See full document

... Sendai virus in HeLa cells suggested that IRF-3 and IRF-7 coordinately induce IFN- ␤ gene tran- scription after infection ...gene induction (23, 27, ...not required for systemic IFN ... See full document

... antibodies against WNV were initially detected 4 days after infection in wild-type animals, which was the same time when high-grade viremia was first detected in ␮MT ...IgM against WNV, derived from ... See full document

... WNV infection. TLR3 stimulation has been shown to promote B-cell activation (2) and cross-priming of virus-specific T cells (17, 22, ...antiviral antibody response leads to rapid and ... See full document

... in antibody responses in Cd22 ⫺/⫺ mice, we next considered if other defects in adaptive immunity might contribute to the increased susceptibility of Cd22 ⫺/⫺ mice to ...WNV infection, CD4 ⫹ or CD8 ⫹ T cells ... See full document

... Type I IFN signaling programs limit infectivity of a wide array of RNA and DNA viruses in cell culture and animals. Because there are at least 14 IFN- ␣ and 1 IFN- ␤ isoforms in addition to multiple other subtypes in ... See full document

... argue against a bacterial ...made West Nile virus infection a strong possibility, which was confirmed by immunoglobulin M (IgM) antibody measure- ments in ... See full document

... The presence of SBV-N specific IgM antibodies was assayed with a capture IgM ELISA. ELISA plates coated with monoclonal anti-ruminant IgM antibodies, recom- binant SBV-N-proteins and monoclonal horseradish per- oxidase ... See full document

... West Nile virus (WNV) is transmitted to vertebrate hosts primarily by infected Culex ...potentiate infection in hosts compared to viral infection by needle ...WNV infection and ... See full document

... after infection, ...IFN response after WNV ...increased infection in peripheral lym- phoid tissues and ...WNV-specific antibody responses were observed throughout the infection time ... See full document

... diarrhea virus, a related member of the Flavi- viridae family, has been shown to activate PERK and to induce eIF2 ␣ phosphorylation ...PERK activation in eIF2 ␣ phos- phorylation in both neuroblastoma cells ... See full document

... information enabling us to estimate the risk of WNV infection and to monitor its evolution during the season. Predictive models could possibly be developed to assess the risk of transmission to humans. This would ... See full document

... core antibody, hepatitis C, Lyme, Saint Louis encephalitis virus, and herpes simplex virus were ...Epstein-Barr virus revealed past ...for antibody to ... See full document

... for virus replication and ...with virus after three passages under selection of either CR4348 (left) or CR4354 (right) on Vero ...mutated virus, and detected with the indicated human IgG1 ... See full document

... Currently, there is no approved specific therapy for WNV or other flaviviruses. We performed here a primary screen of 80,000 small molecule compounds from a commercial library with a cell-based replicon assay that ... See full document