[PDF] Top 20 Complete Transcription by the Transcriptase of Vesicular Stomatitis Virus
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Complete Transcription by the Transcriptase of Vesicular Stomatitis Virus
... The replicating complexes of template and product RNA, generated in vitro by VSV transcriptase, exhibit a mass parity between product and template molecules.. Purified product RNA, after[r] ... See full document
5
Rebinding of transcriptase components (L and NS proteins) to the nucleocapsid template of vesicular stomatitis virus.
... d Purified template and solubilized proteins prepared from other virus samples were recombined under saturating conditions in a standard binding assay.. Downloaded from http://jvi.asm.or[r] ... See full document
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Inhibition of viral transcriptase by immunoglobulin directed against the nucleocapsid NS protein of vesicular stomatitis virus.
... Comparative indirect radioimmunoprecipitation by anti-NS,,,d and anti-whole VS,I,d virion sera of N and NS proteins from cytoplasmic extract and of N protein from nucleocapsid extract Cy[r] ... See full document
10
Effect of Temperature-Sensitive Mutation on Activity of the RNA Transcriptase of Vesicular Stomatitis Virus New Jersey
... The heat stability of the mutant ts Bl was similar to that of wild-type virus, and in vitro RNA synthesis was fully restored when the temperature was lowered to 310C after 30 min of prei[r] ... See full document
9
Isolation of an Infectious Ribonucleoprotein from Vesicular Stomatitis Virus Containing an Active RNA Transcriptase
... Here we describe the purification from VSV particles of a ribonucleoprotein complex TNP with RNA transcriptase activity from which the proteins associated with the outer coat of the viru[r] ... See full document
8
Initiation and Direction of RNA Transcription by Vesicular Stomatitis Virus Virion Transcriptase
... 11, 1973 INITIATION AND DIRECTION OF RNA TRANSCRIPTION plate-product complexes and all the initial transcriptase activity but no free product RNA species or 12P-a-UTP 9, were used to ini[r] ... See full document
15
Dissociation of Vesicular Stomatitis Virus and Relation of the Virion Proteins to the Viral Transcriptase
... After separation, the PEG phase was found to contain only the glycoprotein G; all other virion proteins, as well as RNA and endogenous polymerase activity, were recovered in the dextran [r] ... See full document
10
Identification of a Minimal Size Requirement for Termination of Vesicular Stomatitis Virus mRNA: Implications for the Mechanism of Transcription
... of transcription by primer ...of transcription, primer extension analyses were performed using an oligonucleotide that an- nealed to positive-sense VSV RNA at residues 10925 to 10909 of the complete ... See full document
9
Polyadenylation of Vesicular Stomatitis Virus mRNA Dictates Efficient Transcription Termination at the Intercistronic Gene Junctions
... Sendai virus (16) and Newcastle disease virus ...terminating transcription of the upstream mRNA and reinitiating the downstream mRNA, the polymer- ase just reads through the intercistronic junction ... See full document
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Role of the membrane (M) protein in endogenous inhibition of in vitro transcription by vesicular stomatitis virus.
... The RNA polymerase 134 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest An endogenous transcriptase inhibitor active at high concentrations of vesicular stomatitis VS vi[r] ... See full document
9
Initiation of vesicular stomatitis virus mutant polR1 transcription internally at the N gene in vitro.
... polR1 virus polymerases copy- ing the N gene is ...wild-type virus, except for slightly longer poly(A) tails ...reverse transcriptase would show band sizes equal to the distance be- tween the primer ... See full document
10
Overlapping Signals for Transcription and Replication at the 3′ Terminus of the Vesicular Stomatitis Virus Genome
... in transcription as well as in replication, whereas the template with deletions of nucleotides 19 to 24 was partially active, suggests that the signals for transcription and replica- tion are contained ... See full document
9
Transcription of vesicular stomatitis virus activated by pardaxin, a fish toxin that permeabilizes the virion membrane.
... The inhibitory effect of M protein was manifested for both Pardaxin-induced and Triton-induced transcription at high concentrations of vesicular stomatitis virions; however, unlike the T[r] ... See full document
5
Location of the Transcription Defect in Group I Temperature-Sensitive Mutants of Vesicular Stomatitis Virus
... In order to locate the temperature-sensitive transcription defect in the mutants, wt and ts mutant virions were fractionated by Triton X-100-high salt solubilizer into a sedimentable rib[r] ... See full document
8
RNA synthesis of vesicular stomatitis virus. X. Transcription and replication by defective interfering particles.
... Although the bulk of intracellular RNA synthesis specific to DI particles is known to be under replicative and not transcriptive control 23, it is not clear whether this small RNA is the[r] ... See full document
10
The vesicular stomatitis virus matrix protein inhibits transcription from the human beta interferon promoter.
... in transcription inhibition, it was like the wt protein in causing cytopathic cell rounding ...stock virus or an artifact produced during the PCR step has yet to be ... See full document
7
In vitro and in vivo inhibition of primary transcription of vesicular stomatitis virus by a defective interfering particle.
... We show that chimeras with proteins of the tsGll and tsG13 mutants were unable to transcribe at the nonpermissive tem172 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest[r] ... See full document
11
Polyadenylation of Vesicular Stomatitis Virus mRNA
... In this report, we demonstrate that the virion RNA does -not contain polyuridylic acid [polyU ] sequences corresponding in length to the polyA in the transcription product, and show that[r] ... See full document
6
ATP dependence of vesicular stomatitis virus transcription initiation and modulation by mutation in the nucleocapsid protein.
... Characterization of 0,y-imido ATP-resistant and control products by size, polyadenylic acid content, frequency of initiation at the 3' end of the template, and readthrough of the leader-[r] ... See full document
8
Transcript Initiation and 5′-End Modifications Are Separable Events during Vesicular Stomatitis Virus Transcription
... vitro transcription reactions in the absence of a methyl donor (4, 19, ...vitro transcription of wild-type GFC/⌬G proceeds normally in the absence of a methyl donor and that these transcripts are not ... See full document
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