[PDF] Top 20 Computational Challenges in High Resolution Cryo Electron Microscopy
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Computational Challenges in High Resolution Cryo Electron Microscopy
... higher resolution in several locations, but not conclusively so (Figure ...CPV cryo-EM image dataset was split into halves and independent "half-maps" were generated by Prec and then again by the ... See full document
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A 2.8-Angstrom-Resolution Cryo-Electron Microscopy Structure of Human Parechovirus 3 in Complex with Fab from a Neutralizing Antibody
... At high resolution, we were able to clearly specify the conserved conforma- tional epitope shared among HPeV3 strains, including solvent-accessible atoms in the interface region, which now includes ... See full document
12
Approaches to altering particle distributions in cryo-electron microscopy sample preparation
... Glycerol is commonly added to buffers to stabilize proteins, for example when freezing samples for storage. For high- resolution cryo-EM studies the inclusion of glycerol should be avoided if at all ... See full document
12
A national facility for biological cryo electron microscopy
... other electron optical ...in cryo-EM single-particle analysis these parameters must be determined for each particle using an iterative computational ...the resolution obtainable from suitable ... See full document
10
A 3.0-Angstrom Resolution Cryo-Electron Microscopy Structure and Antigenic Sites of Coxsackievirus A6-Like Particles
... viral challenges (19, 20), indicating that CVA6 VLPs represent an excellent CVA6 vaccine ...by cryo-electron microscopy (cryo-EM) single-particle ... See full document
14
Antigenic and Cryo-Electron Microscopy Structure Analysis of a Chimeric Sapovirus Capsid
... (8.5-Å) cryo-electron microscopy (cryo- EM) structure of a chimeric VLP and developed a VP1 homology ...The cryo-EM structure revealed that the P domain dimers were raised slightly (⬃5 ... See full document
12
Five of Five VHHs Neutralizing Poliovirus Bind the Receptor-Binding Site
... Cryo-electron microscopy. Samples for cryo-electron microscopy were prepared as described by Schotte et ...G2 electron microscope (FEI ...the resolution regimes ... See full document
10
Cryo electron microscopy to determine the structure of macromolecular complexes
... of high-speed detection is that it enables electron counting within a reasonable total exposure ...optical microscopy, individ- ual frames are recorded with very sparse electron events, so ... See full document
9
Recent advances in retroviruses via cryo electron microscopy
... through computational models [85] and is currently supported by the available high-resolution reconstructions obtained from multiple mature virions and [87, ... See full document
10
3D structure of individual nanocrystals in solution by electron microscopy
... particle cryo-electron microscopy (cryo-EM) is a common method for the determination of 3D structures in biological ...by computational methods (11). Single-particle cryo-EM has ... See full document
22
Cryo-electron microscopy structure of yeast Ty retrotransposon virus-like particles.
... The T 5 3 and T 5 4 particle reconstructions presented here provide the first structures for a retroviral core-like particle. It was expected that large holes ( ; 30 Å in diameter) would be found penetrating the surfaces ... See full document
6
High-Resolution Cryo-Electron Microscopy Structures of Murine Norovirus 1 and Rabbit Hemorrhagic Disease Virus Reveal Marked Flexibility in the Receptor Binding Domains
... Using the structure of rNV VLP P domains for modeling, the rRHDV P domains are lifted off the surface of the shell, but not rotated as with MNV-1. This places the bottom edge of the A subunit P1 domain near the S domain ... See full document
6
Cryo-Electron Microscopy Structure of Porcine Deltacoronavirus Spike Protein in the Prefusion State
... For data processing, the recorded movies were corrected for beam-induced motion using Motion- Cor2 (47). The final image was bin averaged to give a pixel size of 1.3 Å. The parameters of the microscope contrast transfer ... See full document
14
Collaborative computational project for electron cryo microscopy
... The Collaborative Computational Project for Electron cryo- Microscopy (CCP-EM) has recently been established. The aims of the project are threefold: to build a coherent cryoEM community which ... See full document
5
The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
... no high-resolution ...of high-resolution density for this ...no high-resolution density, in the unsharpened map there is continu- ous density that encompasses the path of the ... See full document
10
Cryo-Electron Microscopy Structure of Lactococcal Siphophage 1358 Virion
... Twenty-four-Å-resolution cryo-EM reconstruction of the 1358 phage baseplate. (A) The 24-Å cryo-EM electron density map of the closed form of the 1358 baseplate together with the two last ... See full document
11
Synthesis, characterization and catalytic performance of well-ordered crystalline heteroatom mesoporous MCM-41
... The mesoporous heteroatom molecular sieve MCM-41s with the crystalline phase were synthesized via a one-step hydrothermal method. The result materials possess a structure which is in the vertical cross directions with a ... See full document
21
Nanouric acid or nanocalcium phosphate as central nidus to induce calcium oxalate stone formation: a high-resolution transmission electron microscopy study on urinary nanocrystallites
... Conclusion: The formation of CaOx stones is closely related both to the properties of urinary nanocrystallites and to the urinary components. The combination of HRTEM, fast Fourier transformation, selected area ... See full document
11
Cryo Electron Microscopy Structure of an Acinetobacter baumannii Multidrug Efflux Pump
... IMPORTANCE Acinetobacter baumannii is a successful human pathogen which has emerged as one of the most problematic and highly antibiotic-resistant Gram- negative bacteria worldwide. Multidrug efflux is a major mechanism ... See full document
13
PHASE TRANSFORMATIONS IN CALCIUM SUBSTITUTED LANTHANUM FERRITE
... the high angle annular dark field (HAADF) detector suggests that the green A-site located between octahedral planes is preferentially substituted by the larger lanthanum (Z=57) over the calcium cation ...the ... See full document
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