[PDF] Top 20 Conformational Differences between Functional Human Immunodeficiency Virus Envelope Glycoprotein Trimers and Stabilized Soluble Trimers

... the differences in conformation between structurally well-characterized HIV-1 Env trimers (sgp140 ...HIV-1. Differences in the antigenic and glycosylation profiles of these Envs, as well as ... See full document

... gp130 trimers was not sufficient to render all of the nonneutralizing gp120 epitopes inaccessible to ...the soluble gp130 trimers are not thought to be available for antibody binding in the context ... See full document

... the soluble, stabilized trimers are derived can influence the elici- tation of primary virus-neutralizing ...The trimers de- rived from the primary YU2 isolate generated better neutral- ... See full document

... on virus neutralization sensitivity. The purified cleaved Env trimers exhibited a hollow architecture with a central void near the trimer ...Envs, stabilized soluble gp140 SOSIP.664 Env ... See full document

... the envelope (Env) of human immunodeficiency virus type 1 (HIV-1) have been relatively ineffective at generating high-titer, long-lasting immune ...Env stabilized with a synthetic ... See full document

... of envelope glycoprotein immunogens that elicit broadly neutralizing antibody responses remains a major challenge for human immunodeficiency virus type 1 (HIV-1) vaccine ...stable ... See full document

... HIV-1 envelope glycoprotein mutants for function in virus entry and cell-to-cell ...whether functional complementation between envelope glycoprotein mutants with defects ... See full document

... The human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins function as a membrane-an- chored trimer of three gp120 exterior glycoproteins and three gp41 transmembrane ...stabilize ... See full document

... cleaved, envelope glyco- protein complexes? The immunoelectron microscopy data of the 2G12/SOS gp140 complex suggest that appropriately di- rected antibodies could strengthen weak oligomeric interac- ...also ... See full document

... glutaraldehyde-mediated conformational fixation that does not occur efficiently in the context of the cleavage-deficient ...relationships between proteolytic matura- tion and glutaraldehyde ...Env ... See full document

... of soluble HIV-1 envelope glycoprotein trimers, uncleaved, soluble gp140 glycoproteins were stabilized by fusion with another trimeric motif derived from T4 bacteriophage ... See full document

... Stabilized SOS trimers form noncovalently and are cleaved. Based on the above results, we focused on the SOSgp140 I559P and I559G variants for further analysis. These pro- teins are designated SOSIP gp140 ... See full document

... in virus control wells (no test ...ADA-based virus lacking both nef and vpu (HIV-1) or coated with recombinant gp120 ...mean. Differences between groups were compared by using the ... See full document

... prime, soluble-protein boost ...gp140 trimers; second, to determine whether bead-immobilized ...to soluble forms of the same protein at eliciting an Ab response in the boosting phase; and third, to ... See full document

... The virus stocks, which are capable of a single round of infection, were harvested 2 days later, aliquoted, and stored at ⫺ ...mutant envelope glycoproteins were ...produce virus stocks with ... See full document

... ABSTRACT Human immunodeficiency virus type 1 (HIV-1) entry into cells is medi- ated by the viral envelope glycoproteins (Env), a trimer of three gp120 exterior gly- coproteins, and three gp41 ... See full document

... is soluble, does not aggregate, and is easier to express and ...the soluble SOSIP gp140 trimer has a very similar appearance to virion-associated Env at the resolution re- ported ...similar ... See full document

... gp160 glycoprotein. This glycoprotein was expressed efficiently on the cell surface and was precipitated by antibodies that recognize conformation-depen- dent gp120 epitopes (29, ...subtle ... See full document

... HCV envelope glycoproteins play an essential role in virion morphogenesis as well as in HCV entry into liver ...HCV glycoprotein functions, in which protein oligomerization often takes ...virion-associated ... See full document

... nonnative trimers. EM images show that the latter are not true trimers but are oligomeric proteins that contain three splayed-out gp120 subunits linked to a central gp41 ECTO core via the uncleaved ... See full document