[PDF] Top 20 Construction of an adenovirus type 7a E1A- vector.
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Construction of an adenovirus type 7a E1A- vector.
... ated by PCR with the CAT reporter gene expressed from the cytomegalovirus (CMV) promoter in a Bluescript plasmid. The left-end Ad7a sequence corresponding to nucleotide positions 1 to 475 includes the left-end origin of ... See full document
6
Replication-Defective Bovine Adenovirus Type 3 as an Expression Vector
... Replication-defective, recombinant adenoviral vectors are widely used for in vivo gene transfer because of the ability of these vectors to enter many different target cells efficiently and to express the transgene (6). ... See full document
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Importance of the Ser-132 phosphorylation site in cell transformation and apoptosis induced by the adenovirus type 5 E1A protein.
... major E1A products than of 289R ...for E1A 289R alone the transforming efficiency was higher with E1B than with activated ...Figure 7A and B show typical foci produced by pXC38 and pXC13, ... See full document
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Development and Assessment of Human Adenovirus Type 11 as a Gene Transfer Vector
... Ad5-based vectors containing the fibers of Ad serotypes 11 and 35 have shown great promise, as they are able to transduce many human cell types, including human hematopoietic pro- genitor cells, human DCs, and primary ... See full document
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Adenovirus type 5 early region 4 is responsible for E1A-induced p53-independent apoptosis.
... from E1A-induced apoptosis, it was thought that, if E4 products were essential for p53-inde- pendent cell death, some difference in long-term cell survival might be observed, and thus, at various times up to 10 ... See full document
9
The Human Adenovirus Type 5 E4orf6/E1B55K E3 Ubiquitin Ligase Complex Enhances E1A Functional Activity
... enhance E1A effects on E2F. In addition to effects on E1A protein levels, we wondered if the interactions of E4orf6 protein with E2F and E1A products might enhance the ability of E1A to ... See full document
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Genetic mapping of a major site of phosphorylation in adenovirus type 2 E1A proteins.
... 2-D phosphotryptic peptide maps were done for the mutant ElA proteins synthesized in HeLa cells infected with d11310, d1311, and dl313 and the parent virus used for the construction of t[r] ... See full document
9
Original Article The construction and characterization of a novel adenovirus vector of artificial microRNA targeting EGFR
... structure of protein and frequent interactions of proteins may result in the ineffectiveness of these agents. Moreover, the mutations of EGFR are one of the important causes of resistance. In this study, we used RNA ... See full document
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The Human Adenovirus Type 5 E4orf6/E1B55K E3 Ubiquitin Ligase Complex Enhances E1A Functional Activity
... expressing E1A 12S/243R, E4orf6, E1B55K, or all or with plasmid DNA expressing E1A 13S/289R, and after 48 h cell extracts were subjected to plaque assays on 293 cells, as described in Materials and ...or ... See full document
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Construction of Metabolically Biotinylated Adenovirus with Deleted Fiber Knob as Targeting Vector
... fused construction could readily be biotinylated in mammalian cells (data not ...wild type fiber gene in hAd5 vector with the 9R or 9RPSTCD modified fiber gene to generate ...viral vector ... See full document
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Adenovirus type 5 E1A induced apoptosis in COX 2 overexpressing breast cancer cells
... the vector control cells, and 58% for the E1A stable transfectants (Figure ...the E1A stable transfectants (Figure ...the E1A trans- fectants than in the corresponding parental or ... See full document
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Expression of adenovirus type 12 E1A gene in monkey cells, using a simian virus 40 vector.
... Solid lines, pBR322 sequences; open bars, SV40 or Adl2 sequences; 0, origin of SV40 DNA replication; shaded regions, SV40 sequences containing the 5' striped regions and 3' crosshatched [r] ... See full document
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Generation of a Conditionally Replicating Adenovirus Based on Targeted Destruction of E1A mRNA by a Cell Type-Specific MicroRNA
... of E1A may be explained by the results of previous studies that have reported the capacity of adenovirus to replicate normally despite strongly reduced levels of E1A ...reduce E1A protein ... See full document
7
Modulation of cyclin gene expression by adenovirus E1A in a cell line with E1A-dependent conditional proliferation.
... As already suggested by the rapid induction of cyclin E mRNA upon activation of ElA-ER in IREE-1 cells, these data indicate that EIA acts as a potent inducer of cyclin E gene expression,[r] ... See full document
9
Hacking the Cell: Network Intrusion and Exploitation by Adenovirus E1A
... novel E1A interacting partners have been shown to contribute to this deregulation of cell cycle ...control. E1A binds to NimA-related protein kinase 9 (Nek9) through its N terminus (188, ...with E1A ... See full document
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The CR1 and CR3 domains of the adenovirus type 5 E1A proteins can independently mediate activation of ATF-2.
... of E1A has been well estab- ...12S E1A in F9 ...12S E1A and wtE1A were approximately ...an E1A-like activity that has been found to be present in these cells and which has a function similar ... See full document
8
Adenovirus E1A Directly Targets the E2F/DP-1 Complex
... between E1A and DP-1, we determined that E1A 13S can activate expression from E2F-regulated promoters in a fashion that does not rely on E1A binding to pRb or other pocket proteins in asynchronously ... See full document
11
Adenovirus 5 E1A-Mediated Suppression of p53 via FUBP1
... Effects of FUBP1 depletion on viral genome replication and gene expression. Since we observed that the depletion of FUBP1 had a modest effect on virus growth, similar to what we have seen for other recently identified ... See full document
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Analysis of trans activation by human papillomavirus type 16 E7 and adenovirus 12S E1A suggests a common mechanism.
... Specifically, each displays oncogenic activity in the form of ras cooperativity, each encodes proteins that can trans-activate transcription as measured by activation of the adenovirus E[r] ... See full document
9
Adenovirus E1A is associated with a serine/threonine protein kinase.
... When the ElA immune complexes from unlabeled 293 cell lysates were incubated in the presence of [_y-32P]ATP and the products of the reaction were analyzed by gel electrophoresis, two pro[r] ... See full document
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