[PDF] Top 20 Control of mRNA stability by the virion host shutoff function of herpes simplex virus.
Has 10000 "Control of mRNA stability by the virion host shutoff function of herpes simplex virus." found on our website. Below are the top 20 most common "Control of mRNA stability by the virion host shutoff function of herpes simplex virus.".
Control of mRNA stability by the virion host shutoff function of herpes simplex virus.
... The first demonstration that the vhsl mutation affects the half-lives of viral as well as host mRNAs was provided by the observation that, following reversal of a cycloheximide block pre[r] ... See full document
10
Small Interfering RNAs That Deplete the Cellular Translation Factor eIF4H Impede mRNA Degradation by the Virion Host Shutoff Protein of Herpes Simplex Virus
... bind host translation initiation factors, includ- ing eIF4H (14, 17, 18) and/or eIF4B ...the mRNA to allow binding of the 40S ribosome subunit and the initiation of cap-dependent scanning ... See full document
10
Picornavirus Internal Ribosome Entry Site Elements Target RNA Cleavage Events Induced by the Herpes Simplex Virus Virion Host Shutoff Protein
... FIG. 2. The pCITE transcript is cleaved immediately downstream of the IRES. (A) Uncapped, internally labeled transcripts of pCITE and pCITE RI/ AvrII (lacking the 5⬘-most 159 nt of the IRES) were added to RRLs containing ... See full document
10
The Virion Host Shutoff Endonuclease (UL41) of Herpes Simplex Virus Interacts with the Cellular Cap-Binding Complex eIF4F
... The virion host shutoff (Vhs) (UL41) endonuclease is a component of herpes simplex (HSV) virions (2, 4, 26, 27, 32, 42) that, during lytic infections, accelerates the turnover of many ... See full document
5
Herpes Simplex Virus 2 Virion Host Shutoff Endoribonuclease Activity Is Required To Disrupt Stress Granule Formation
... with herpes simplex virus 2 (HSV-2) have a disruption in their ability to form SGs in response to treatment with arsenite, an inducer of oxidative stress ...defective virion host ... See full document
13
The herpes simplex virus virion host shutoff function.
... The lack of host shutoff at an input MOI of 2 PFU of KOS virus in the presence of dactinomycin per cell was also observed in additional experiments in which the level of host cell protei[r] ... See full document
6
The Herpes Simplex Virus Host Shutoff RNase Degrades Cellular and Viral mRNAs Made before Infection but Not Viral mRNA Made after Infection
... A herpes simplex virus tegument protein brought into the cell during infection and designated the virion host shutoff protein (VHS) is an endoribonuclease that degrades ...viral ... See full document
7
The Splicing History of an mRNA Affects Its Level of Translation and Sensitivity to Cleavage by the Virion Host Shutoff Endonuclease during Herpes Simplex Virus Infections
... lytic herpes simplex virus (HSV) infections, the virion host shutoff (Vhs) (UL41) endoribonuclease degrades many cellu- lar and viral ...reporter mRNA or RLuc constructs ... See full document
13
The virion host shutoff protein of herpes simplex virus type 1: messenger ribonucleolytic activity in vitro.
... protein-induced mRNA degradation, our laboratory and the Read laboratory have re- produced the early destabilization phenomenon in cell extracts (24, ...degraded. mRNA destabilization is not observed with ... See full document
9
The Effect of Herpes Simplex Virus Virion Host Shutoff Gene- a New Suicide Gene- on Tumor Cells
... This study was initiated to investigate the effect of Vhs on tumor cells. The Vhs gene was amplified from HSV-2 genome and cloned into the pcDNA3 plasmid. The pcDNA3 plasmid contains a very strong cytomegalovirus ... See full document
5
Selective Ablation of Virion Host Shutoff Protein RNase Activity Attenuates Herpes Simplex Virus 2 in Mice
... Coimmunoprecipitation. Vero cells in 6-well plates were mock transfected or cotransfected with 1 g each of a plasmid expressing HSV-2 VP22 [pcDNA- VP22(2)], VP16 (pcDNA-VP16), and vhs (pcDNA-333vhsHA or pcDNA- D215NHA), ... See full document
12
mRNA Decay during Herpes Simplex Virus (HSV) Infections: Protein-Protein Interactions Involving the HSV Virion Host Shutoff Protein and Translation Factors eIF4H and eIF4A
... (11). They also found that recombinant eIF4B and eIF4H both stimulated the Vhs-dependent endonuclease activity present in whole-cell extracts of yeast engineered to express Vhs (11). Previous studies of eIF4H deletion ... See full document
14
A Single Amino Acid Substitution in Herpes Simplex Virus Type 1 VP16 Inhibits Binding to the Virion Host Shutoff Protein and Is Incompatible with Virus Growth
... transcription of IE promoters. (A) Transcriptional activation by VP16 mutant proteins. COS-1 cells was transfected with mammalian expres- sion vectors for VP16 harboring the indicated point mutations along with the ... See full document
11
Improved cell survival by the reduction of immediate-early gene expression in replication-defective mutants of herpes simplex virus type 1 but not by mutation of the virion host shutoff function.
... 10 0022-538X/94/$04.00+0 Copyright C 1994, American Society for Microbiology Improved Cell Survival by the Reduction of Immediate-Early Gene Expression in Replication-Defective Mutants o[r] ... See full document
16
Mutational analysis of the herpes simplex virus virion host shutoff protein: evidence that vhs functions in the absence of other viral proteins.
... Herpes simplex virus (HSV) virions contain one or more factors that trigger rapid shutoff of host protein synthesis and accelerated decay of cellular and viral mRNAs in infected ...the ... See full document
9
mRNA Decay during Herpes Simplex Virus (HSV) Infections: Mutations That Affect Translation of an mRNA Influence the Sites at Which It Is Cleaved by the HSV Virion Host Shutoff (Vhs) Protein
... pBK2 mRNA, a modified form of the HSV-1 thymidine kinase (TK) mRNA, beginning with cleavages at sites in the first 250 nucleo- tides, including a number near the start codon and in the 5= un- translated ... See full document
16
Herpes Simplex Virus Virion Host Shutoff Protein Requires a Mammalian Factor for Efficient In Vitro Endoribonuclease Activity
... the control of the galac- tose-inducible GAL10 promoter (Yvhs); as controls, we also derived strains bearing the empty expression vector (YGAL) and a vector specifying the inactive vhs1 mutant form of vhs ...the ... See full document
14
The Virion Host Shutoff Protein (UL41) of Herpes Simplex Virus 1 Is an Endoribonuclease with a Substrate Specificity Similar to That of RNase A
... S-transferase–virion host shutoff (GST-vhs) protein exhibited endoribonucleolytic activity in in vitro assays using as substrates in vitro-transcribed regions of IEX-1 ... See full document
5
Therapeutic Immunization with a Virion Host Shutoff-Defective, Replication-Incompetent Herpes Simplex Virus Type 1 Strain Limits Recurrent Herpetic Ocular Infection
... of virus shedding in previously infected mice ...mutant virus is on a par with that of the replication-competent BGS41 vaccine strain, as was previously seen for prophylactic immunization ...to ... See full document
11
Inhibition of human immunodeficiency virus replication by the herpes simplex virus virion host shutoff protein.
... viral mRNA splicing in infected ...viral mRNA from the nucleus into the cytoplasm and increasing the ratio of unspliced and singly spliced mRNA to doubly spliced mRNA ...of host factors ... See full document
7
Related subjects