[PDF] Top 20 Coronavirus transcription: subgenomic mouse hepatitis virus replicative intermediates function in RNA synthesis.
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Coronavirus transcription: subgenomic mouse hepatitis virus replicative intermediates function in RNA synthesis.
... Because only genome-length negative-strand templates were found in mouse hepatitis virus MHV-infected cells and in replicative intermediates RIs obtained from MHV-infected cells and beca[r] ... See full document
7
Subgenomic RNA synthesis directed by a synthetic defective interfering RNA of mouse hepatitis virus: a study of coronavirus transcription initiation.
... However, the results obtained for the promoter mutants Bi and C3, both containing the same A->C substitution Table 2, seem difficult to interpret in terms of base pairing: introduction o[r] ... See full document
11
Analysis of a recombinant mouse hepatitis virus expressing a foreign gene reveals a novel aspect of coronavirus transcription.
... of coronavirus transcription postulated that (positive-strand) subgenomic mRNAs were generated by pausing of the polymerase near the end of the leader sequence followed by detachment and reattachment ... See full document
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Mechanism of coronavirus transcription: duration of primary transcription initiation activity and effects of subgenomic RNA transcription on RNA replication.
... This model is consistent with most previously described observations; subgenomic DI RNA synthesis does not occur in subgenomic DI RNA-transfected, MHV-infected cells 20, subgenomic RI RN[r] ... See full document
8
The Putative Helicase of the Coronavirus Mouse Hepatitis Virus Is Processed from the Replicase Gene Polyprotein and Localizes in Complexes That Are Active in Viral RNA Synthesis
... viral RNA, but the function of Hel in the complex during RNA synthesis remains to be de- ...in RNA synthesis and replication complex ...can function in the highly complex ... See full document
10
Equine arteritis virus subgenomic mRNA synthesis: analysis of leader-body junctions and replicative-form RNAs.
... underlying transcription mechanism may be imprecise (3, ...precursor RNA, as has been suggested in the past (35), and that at least late in infection their synthesis is largely independent from that ... See full document
8
Polypyrimidine Tract-Binding Protein Binds to the Leader RNA of Mouse Hepatitis Virus and Serves as a Regulator of Viral Transcription
... Mouse hepatitis virus (MHV) is a single-stranded, positive- sense RNA virus and belongs to the Coronaviridae ...an RNA genome of approximately 31 kb (22), which encodes seven to ... See full document
6
Interactions between the cytoplasmic proteins and the intergenic (promoter) sequence of mouse hepatitis virus RNA: correlation with the amounts of subgenomic mRNA transcribed.
... Among RNA viruses, coronavirus, as exemplified by mouse hepatitis virus (MHV), employs unique strategies for RNA replication and transcription, probably as a consequence ... See full document
8
Mouse Hepatitis Virus Stem-Loop 4 Functions as a Spacer Element Required To Drive Subgenomic RNA Synthesis
... directing subgenomic RNA ...genomic RNA, as described in the negative-strand discon- tinuous transcription model defined by Zuniga et ...MHV RNA leader TRS, and heterogeneous nuclear ... See full document
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Subgenomic Negative-Strand RNA Function during Mouse Hepatitis Virus Infection
... negative-strand synthesis and hotter pools of nucleotide precursors, this could also represent RF RNA structures which had burned out and were accumulating during ...RF RNA should decrease over time ... See full document
8
The 3' untranslated region of coronavirus RNA is required for subgenomic mRNA transcription from a defective interfering RNA.
... of mouse hepatitis virus (MHV) genomic RNA contains a recognition sequence (55 nucleotides [nt]) required for minus-strand RNA ...in subgenomic mRNA transcription, we have ... See full document
5
The Leader RNA of Coronavirus Mouse Hepatitis Virus Contains an Enhancer-Like Element for Subgenomic mRNA Transcription
... Mouse hepatitis virus (MHV) is the prototype of Murine coronavirus, a member of the family ...single-stranded RNA genome 31 kb long, which encodes seven to eight genes (16, 18, ... See full document
10
Highly Permissive Cell Lines for Subgenomic and Genomic Hepatitis C Virus RNA Replication
... of subgenomic replicons lacking neo as well as full-length HCV genome ...deleterious RNA secondary structures (20, 26) or RNA replication, by disrupting RNA elements that lie in the HCV 5⬘ NTR ... See full document
14
Modeling Subgenomic Hepatitis C Virus RNA Kinetics during Treatment with Alpha Interferon
... sg1b RNA decline ...HCV RNA present in these cells does contribute to the number of HCV RNA copies present in the cultures throughout the ...viral RNA dynamics during IFN- ␣ ... See full document
8
Synthesis of virus-specific polypeptides and genomic RNA during the replicative cycle of Pichinde virus.
... The nucleoprotein of Pichinde virus represents approximately 85% of both labeled virion protein and viral proteins immunoprecipitable from lysates of infected cells incubated in the pres[r] ... See full document
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SYNCRIP, a Member of the Heterogeneous Nuclear Ribonucleoprotein Family, Is Involved in Mouse Hepatitis Virus RNA Synthesis
... of transcription factors (34); it was also observed previ- ously with the full-length PTB in MHV RNA transcription ...MHV transcription (29), the domi- nant-negative effect observed with F-SYN ... See full document
10
Coronavirus minus-strand RNA synthesis and effect of cycloheximide on coronavirus RNA synthesis.
... The replication of genomesized plus- and minus-strand RNA by the rhabdovirus vesicular stomatitis virus required continued synthesis of viral nucleocapsid protein 3, 4, 16, 32, but the t[r] ... See full document
7
Hepatitis C Virus Subgenomic Replicon Requires an Active NS3 RNA Helicase
... cellular RNA was extracted from ...cellular RNA using SuperScript II RNase H- reverse transcriptase (RT) (Invitrogen) in the presence of 100 nM of one of two primers complementary to the HCV 5 ⬘ ... See full document
8
The 5' nontranslated region of potato virus X RNA affects both genomic and subgenomic RNA synthesis.
... potato virus X (PVX) ...for RNA production by S1 nuclease protec- tion ...of RNA levels in protoplasts inoculated with PVX clones containing deletions or an insertion in their 5 * ... See full document
8
Structure and Functional Relevance of a Transcription-Regulating Sequence Involved in Coronavirus Discontinuous RNA Synthesis
... Altogether, the analysis of wild-type and mutant sequences indicated that the TRS-L needed to form a well-defined hair- pin structure, with a specific optimal thermal stability, for ef- ficient CoV replication and ... See full document
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