[PDF] Top 20 CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
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CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
... for genome engineering (Hsu et ...in Caenorhabditis elegans and other ...current CRISPR-based approaches (Arribere et ...these methods and those based on the Mos1 ... See full document
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Rapid and Precise Engineering of the Caenorhabditis elegans Genome with Lethal Mutation Co-Conversion and Inactivation of NHEJ Repair
... To confirm that pha-1(ts) co-conversion, PCR-generated sgRNAs templates, and NHEJ inactivation were effective on other loci, I attempted to introduce a 23FLAG epitope in nhr-25, a broadly expressed nuclear hormone ... See full document
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Engineering and Application of cGAL, a GAL4 Bipartite Expression System for Caenorhabditis elegans
... function. Caenorhabditis elegans is a powerful genetic model organism, with a variety of transgenesis methods available to ...all methods suffer from issues of reproducibility, reusability, ... See full document
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Exciting Prospects for Precise Engineering of Caenorhabditis elegans Genomes with CRISPR/Cas9
... C. elegans, genome-editing techniques have until re- cently primarily been based on transposons, which cause DSBs upon their excision (Figure ...were based on Tc1 exci- sion (Barrett et ... See full document
8
CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae
... metabolic engineering. Several powerful approaches, either plasmid- or PCR- based, have been demonstrated for genome integra- tions using selection ...Such methods typically use active ... See full document
11
Heritable Custom Genomic Modifications in Caenorhabditis elegans via a CRISPR–Cas9 System
... ENOME engineering has proven to be a useful tool in biological ...nematode Caenorhabditis elegans, creating heritable muta- tions had until recently involved random mutagenesis, and the insertion of ... See full document
14
High Efficiency, Homology-Directed Genome Editing in Caenorhabditis elegans Using CRISPR-Cas9 Ribonucleoprotein Complexes
... ABSTRACT Homology-directed repair (HDR) of breaks induced by the RNA-programmed nuclease Cas9 has become a popular method for genome editing in several organisms. Most HDR protocols rely on plasmid-based ... See full document
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CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans
... URRENT methods to generate mutations in the genome of Caenorhabditis elegans, including chemical mutagen- esis and imprecise excision of transposons, all rely on recovering mutations in ... See full document
11
Transgene-Free Genome Editing in Caenorhabditis elegans Using CRISPR-Cas
... Methods based on CRISPR-directed nuclease activity offer the potential for maximally convenient engineering of target specificity to effect changes to the ...the CRISPR RNA (crRNA) and ... See full document
6
Targeted genome engineering in Caenorhabditis elegans
... activator-like; CRISPR: clustered regularly interspaced short palindromic repeats; Cas: CRISPR-associated proteins; Cas9: native Cas9 nuclease; crRNA: CRISPR RNA; pre-crRNA: precursor CRISPR ... See full document
10
A Co-CRISPR Strategy for Efficient Genome Editing in Caenorhabditis elegans
... for CRISPR-induced HR, we decided to employ the unc-119(+) marker as well as a new worm antibiotic-resistance marker expressing the bacterial BSD gene as selectable markers and the avr-15 gene as a counter- ... See full document
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CRISPR Genome Engineering for Human Pluripotent Stem Cell Research
... iPSCs is dermal fibroblasts [44], stemming from their ease of access and programmability efficiency, with peripheral blood [45], cord blood [46], and Epstein-Barr virus-immortalized B-cell lines also emerging as ... See full document
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Advances in Engineering the Fly Genome with the CRISPR-Cas System
... fly genome is significantly changing the course of research in ...Cas9-mediated genome engineering and active-genetic strategies are likely to have a transformative impact on genetics as we currently ... See full document
18
Ushering in CRISPR/Cas Mediated Genome Engineering for Crops
... CGAT, CRISPR-PLANT, CRISPR-P and CRISPR-P ...utilised CRISPR/Cas9 system to generate herbicide tolerant maize, glyphosate tolerant rice and is presently utilizing this tool for tomato and ... See full document
8
Shifting patterns of natural variation in the nuclear genome of caenorhabditis elegans
... C. elegans poly- morphic spectrum has not been accomplished until ...C. elegans have not focused on examining the patterns of variation that we present here, but have instead focused on other important pat- ... See full document
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Genome Engineering of Drosophila with the CRISPR RNA-Guided Cas9 Nuclease
... G ENETIC and molecular techniques to manipulate the genomes of model organisms are invaluable tools for understanding gene function. In Drosophila, chemical and insertional mutagenesis are powerful and widely utilized ... See full document
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SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification in Caenorhabditis elegans
... To validate cell specificity of FLP-on GFP induction, we expressed FLP under the control of a variety of more restrictive promoters and assayed the induction of SNT-1::GFP. First, we examined conditional tagging of SNT-1 ... See full document
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Proteomics Profiling and Functional Characterization of Caenorhabditis elegans Excreted/Secreted Proteins
... C. elegans ESPs composition is a key to understand how nematodes respond and adapt to fast changing environment and to elucidate the strategies to survive and reproduce in natural ... See full document
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Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9
... gondii genome, and the Cas9 nuclease has been replaced with a pyrimethamine resistance ...to CRISPR/Cas9 is multifactorial, including a modest effect from nuclease expression that is significantly ... See full document
8
A Multiplex CRISPR/Cas9 System for Use as an Anti-BmNPV Therapeutic
... the CRISPR/Cas9 system to efficiently knock out multiple target genes [26, ...one-vector CRISPR/Cas9 system that can not only screen and identify the key genes of BmNPV systemic infection process, but also ... See full document
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