[PDF] Top 20 Cytotoxicity of a replication-defective mutant of herpes simplex virus type 1.

... It is also possible that early gene expression in cells infected by HSV-1 strains which have an intact IE 3 gene contributes to the induction of CPE, particularly since IE 3-expressing s[r] ... See full document

... Experiments using plasmid pSA1 demonstrated that KUL25NS stably packaged replicated amplicon DNA rela- tively more efficiently than it packaged its own genome and in amounts approaching that encapsidated in the presence ... See full document

... As anticipated, UL14D failed to produce the UL14 protein. It is possible in principle for UL14D to produce a C-terminally truncated form of the UL14 protein containing the N-terminal 96 residues. This protein was not ... See full document

... null mutant RGKO and GKO mice (5; ...Table 1, the mortality in female RGKO and GKO mice was 8% (the same mortality as in the female 129/Sv//Ev controls), whereas the mortality rates in both male null ... See full document

... deletion mutant appeared to establish (or maintain) latency with approximately 19 to 36% of the efficiency of the rescued virus, as judged by semiquantitative PCR analysis of viral ...this type of ... See full document

... Examination of the distribution of substituted bases pro- vided information regarding the potential hot spot(s) of mu- tations replicated by Pol. Figure 4 depicts the locations of all simple mutations characterized in ... See full document

... Although HSV-1 remained largely undetected in culture medium until 48 h after heat stress, replication had initiated in neurons by 24 h after heat stress. The frequency of detecting HSV-1 in culture ... See full document

... In addition, survival rates determined from plating efficiencies of the various cell mutants after incubations at 39.5°C for the time periods corresponding 42 Downloaded from http://jvi.[r] ... See full document

... noncompetitive inhibitor of nitric oxide synthetase substantially reduced the antiviral effect of activated RAW macrophages. S-nitroso-L-acetyl penicillamine (SNAP) is a donor of NO and was added to the media of infected ... See full document

... These data are consistent with the view that the genes ULS, UL8, UL9, UL42, and ULS2, like the genes encoding ICP8 and polymerase 12, 15, are all delayed-early genes, as expected for our[r] ... See full document

... Figure 8 summarizes the cleavages mapped in vivo in this DISCUSSION Topoisomerase II consensus sequences are distributed throughout the HSV-1 genome, and some of these sites are recogniz[r] ... See full document

... Location of the gene for gF on the HSV-2 genome and the genes for gC 9, 14 and TK 17, 32 on the HSV-1 genome and a summary of the results obtained from screening AraTr viruses that conta[r] ... See full document

... of virus shedding in previously infected mice ...the replication-incompetent double mutant virus is on a par with that of the replication-competent BGS41 vaccine strain, as was ... See full document

... HSV replication complex to the AAV ...(Fig. 1, lane ...DNA replication (Fig. 1, lane ...(Fig. 1, lane ...was 1:5, which corresponds approxi- mately to ratio of the molecular ... See full document

... In stationary state, 3T6 cells, 1624 and 1624.5 behaved similarly to 1716 and failed to grow whereas 1622 and 1625 grew. In this cell line, replication of ICP34.5 null mutant (1716) has been previously ... See full document

... 3528 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest During the course of experiments designed to isolate deletion mutants of herpes simplex virus type 1 in the encodin[r] ... See full document

... cell type in the cornea as HSK ...elevated virus growth in the neutrophil-depleted hosts could produce more tissue dam- age, thereby compensating for the loss of putative destructive neutrophil ... See full document

... The cells used in this study were baby hamster kidney cells BHK-21; 5-bromo2'-deoxyuridine BUdR-resistant BHK cells BUBHK, which express no cellular TK; and PyY/TG/ 649 Downloaded from h[r] ... See full document

... 25 take the view that the accumulation of virus particles at the cell surface by interaction with heparin sulfate is followed by the interaction of the virus with a saturable receptor in[r] ... See full document

... Herpes simplex virus type 1 (HSV-1) produces oral lesions, encephalitis, keratitis, and severe infections in the immunocompromised ...genital herpes, a disease that is ... See full document