[PDF] Top 20 Detection of Plasmodium Infection by the illumigene Malaria Assay Compared to Reference Microscopy and Real Time PCR
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Detection of Plasmodium Infection by the illumigene Malaria Assay Compared to Reference Microscopy and Real Time PCR
... and illumigene malaria assay. Microscopy was performed using standard Giemsa- stained thick and thin peripheral films at the time of initial diagnosis ...the illumigene ... See full document
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Epidemiology of Plasmodium infections in Flores Island, Indonesia using real time PCR
... severe malaria, other studies were unable to link ABO blood groups to the incidence of malaria ...AB compared to subjects with the other blood ...microscopic Plasmodium infections were found ... See full document
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Sequence Based Optimization of a Quantitative Real Time PCR Assay for Detection of Plasmodium ovale and Plasmodium malariae
... and PCR assays are the mainstays of malaria diagnostics in resourced settings; how- ever, microscopy remains the gold ...on microscopy present challenges due to the technical expertise ... See full document
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Evaluation of FRET real time PCR assay for rapid detection and differentiation of Plasmodium species in returning travellers and migrants
... the detection of Plasmodium ...other malaria parasite species ...the malaria attacks by the ...mixed infection, the ability to provide accurate species identification in the same sample ... See full document
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High Throughput Pooling and Real Time PCR Based Strategy for Malaria Detection
... in real-time PCR assays: in the subset of microscopy- negative samples with a predicted low prevalence of para- sitemia, the estimated cost saving of pooling, compared with the cost of ... See full document
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Development of a Real Time PCR Assay for Detection of Plasmodium falciparum, Plasmodium vivax, and Plasmodium ovale for Routine Clinical Diagnosis
... TaqMan-based real-time PCR qualitative assay for the detection of three species of malaria parasites— Plasmodium falciparum, ...where malaria is endemic and who ... See full document
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Real Time PCR for Detection and Identification of Plasmodium spp
... the assay, DNA was isolated from eight blood specimens known to contain either ...than malaria diagnosis and 158 blinded samples from patients with fever and a history of recent travel within a region where ... See full document
6
Diagnostic performance of the loop mediated isothermal amplification (LAMP) based illumigene® malaria assay in a non endemic region
... for malaria confirmation to the Central Laboratory of Clinical Biology of ITM (the Belgian ref- erence laboratory for Plasmodium, accredited according to ...by real-time PCR (Cnops et ... See full document
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Simple Real Time PCR and Amplicon Sequencing Method for Identification of Plasmodium Species in Human Whole Blood
... Accurate Plasmodium species identification has therapy implications for ...primaquine. Compared to microscopy, nucleic acid tests have improved specificity for species identification and higher ... See full document
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Real Time Quantitative PCR for Determining the Burden of Plasmodium falciparum Parasites during Pregnancy and Infancy
... Malaria infection in pregnant women can result in intrauter- ine growth retardation, which results in the birth of under- weight babies, thereby increasing their risk for infant death (18, ...placental ... See full document
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Comparison of real time PCR and microscopy for malaria parasite detection in Malawian pregnant women
... pfldh PCR assay can theoretically quantify the amount of ...the assay were, in fact, “submicro- scopic”, they may also be the result of poorly-sensitive ...Moreover, microscopy can be an ... See full document
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A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... with malaria to Kapit Hospital, Sarawak from July 2006 to February 2008 for a study aimed at deter- mining the clinical and laboratory features of knowlesi malaria ...the Malaria Research Centre, ... See full document
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Real Time PCR Assay for Diagnosis of Entamoeba histolytica Infection
... Real-time-PCR assay. The oligonucleotide primers and molecular-beacon probe were designed (using Oligo software from Beacon Designer-2.1; Bio-Rad) to specifically amplify a 134-bp fragment ... See full document
5
Real Time PCR for Diagnosis of Human Bocavirus Infections and Phylogenetic Analysis
... conventional PCR methods with agarose gel electro- ...hBoV detection, we have established a real-time PCR ...this assay proved to be sensitive, spe- cific, and reliable for hBoV ... See full document
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Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... TaqMan real-time RT-PCR assay developed in the present study offers a useful option for laborator- ies requiring a high-throughput testing ...simultaneous detection of H5 HA and N6 NA ... See full document
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Host Range and Vertical Transmission of Cyprinid herpesvirus 2
... CyHV-2 infection and enormous financial losses, studies involved in CyHV- 2 have been given additional ...and detection of CyHV-2(Goodwin et ...(LAMP) assay, PCR, real-time ... See full document
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Novel real-time PCR detection assay for Brucella suis
... Real time PCR products were analysed by capillary gel electrophoresis using the QIAxcel DNA high resolution kit (1200) with an analytical range of 15 bp to 10 kb on the QIAxcel Advanced system ... See full document
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Multiplex Real Time PCR Assay for Simultaneous Detection of Acanthamoeba spp , Balamuthia mandrillaris, and Naegleria fowleri
... triplex real-time TaqMan PCR assay that can simultaneously identify Acanthamoeba ...same PCR vessel. The assay was validated with 22 well-characterized amebic strains harvested ... See full document
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Comparison of BD GeneOhm Cdiff Real Time PCR Assay with a Two Step Algorithm and a Toxin A/B Enzyme Linked Immunosorbent Assay for Diagnosis of Toxigenic Clostridium difficile Infection
... difficile infection (CDI) is emerging as the most common infectious cause of nosocomial diarrhea, yet sensitive and specific commercially available diagnostic tests with rapid turnaround times are lacking ... See full document
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Validation of a pan orthopox real time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood
... The pan-orthopox PCR assay has been previously described by [4], with the exception of quantitation strategy utilizing the aforementioned standard curve. Each LightCycler reaction was comprised of 5.0 μl of ... See full document
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