[PDF] Top 20 Detection of receptor-specific murine leukemia virus binding to cells by immunofluorescence analysis.
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Detection of receptor-specific murine leukemia virus binding to cells by immunofluorescence analysis.
... To determine whether the virus binding detected by this method represents a specific viral envelope-cell surface receptor interaction, we compared the binding specificity of ecotropic an[r] ... See full document
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G100R Mutation within 4070A Murine Leukemia Virus Env Increases Virus Receptor Binding, Kinetics of Entry, and Viral Transduction Efficiency
... mediate virus binding ...increased binding over ⌬H36 alone ...4070A virus. (A) Assays of binding of the G100R Env virus on D17 and NIH 3T3 ...D17/gag-pol/⌿GIP cells to ... See full document
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Identification of a subdomain in the Moloney murine leukemia virus envelope protein involved in receptor binding.
... the receptor-binding domain of Moloney murine leukemia virus envelope in order to identify residues involved in receptor ...binding. Analysis of mutations in the ... See full document
7
Receptor-binding properties of a purified fragment of the 4070A amphotropic murine leukemia virus envelope glycoprotein.
... Efficient binding and internaliza- tion of retrovirus particles may also require cooperation be- tween several Ram-1 molecules, which may not be necessary for the natural function of these molecules as phosphate ... See full document
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GRB2 Interaction with the Ecotropic Murine Leukemia Virus Receptor, mCAT-1, Controls Virus Entry and Is Stimulated by Virus Binding
... HEK293 cells, and virus infection was measured by FACS ...(38). Cells expressing the YFP-tagged GRB2 R86K construct showed a 6-fold reduction in infection compared to cells expressing YFP ... See full document
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Role of Variable Regions A and B in Receptor Binding Domain of Amphotropic Murine Leukemia Virus Envelope Protein
... of murine leukemia virus (MuLV) into cells is initiated by an interaction between the envelope glycoprotein and the host cell ...Another murine retrovirus, Mus dunni endogenous ... See full document
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Initial Binding of Murine Leukemia Virus Particles to Cells Does Not Require Specific Env-Receptor Interaction
... virion binding to cells. The kinetics of virus binding to TE671 cells in the presence and absence of specific receptor-Env interaction was ...The virus-cell associ- ... See full document
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Properties of the Naturally Occurring Soluble Surface Glycoprotein of Ecotropic Murine Leukemia Virus: Binding Specificity and Possible Conformational Change after Binding to Receptor
... the cells with 2 g of biotinyl- ated MAb282 or biotinylated MAb4D2 for 30 min and then with ...the cells were fixed in PBS containing 1% paraformalde- hyde prior to the flow cytometric ...3T3 cells ... See full document
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A Hydrophobic Patch in Ecotropic Murine Leukemia Virus Envelope Protein Is the Putative Binding Site for a Critical Tyrosine Residue on the Cellular Receptor
... 3T3 cells bearing the endogenous ecotropic MLV receptor (stippled bars) and human 293 cells expressing the exogenous receptor (black ...of cells trans- fected with a virus genome ... See full document
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Linkage of Reduced Receptor Affinity and Superinfection to Pathogenesis of TR1.3 Murine Leukemia Virus
... TR1.3-infected cells. The diminu- tion of available surface receptor by Env is an important com- ponent in controlling retrovirus cytopathology and syncytium ...relative binding of ...ulate ... See full document
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Receptor-binding domain of murine leukemia virus envelope glycoproteins.
... fluoride), cells were disrupted under the same conditions, and both were clarified by ...to analysis. For pulse-chase experiments, cells were labeled for 40 min with 150 mCi of [ ... See full document
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Characterization of murine-specific leukemia virus receptor from L cells.
... Specific binding to the precipitated membrane protein 7 ,ug/,ul was determined by subtracting the nonspecific binding to heat-treated precipitate from the total bound amount of _251-labe[r] ... See full document
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Binding kinetics of ecotropic (Moloney) murine leukemia retrovirus with NIH 3T3 cells.
... from virus particles (2, 33) or secreted directly from producer cells as in the case of simian immuno- deficiency virus ...the virus supernatant could confound our analysis of ... See full document
6
Intracellular assembly and budding of the Murine Leukemia Virus in infected cells
... EM analysis were conducted in derivative human HT1080 cells that expressed Gag/Gag-Pol alone (HT-Fly ...MVB. Analysis of several cell sections revealed that Gag-VLP budded more frequently at the ... See full document
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Core-Binding Factor Influences the Disease Specificity of Moloney Murine Leukemia Virus
... T cells, core sites with a range of affinities for the Runt domain were engineered into the Moloney MLV enhancer, and tran- scriptional activity was measured in several T-cell ...proteins binding to ... See full document
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The receptors for gibbon ape leukemia virus and amphotropic murine leukemia virus are not downregulated in productively infected cells
... identify receptor-ligand interactions was used to further validate the association of SLC20A1- HA and GALV ...MDTFSLC20A1-HA cells in suspension were exposed to GALVRBD-V5 and then incubated with ... See full document
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Analysis of the receptor-binding site of murine coronavirus spike protein.
... is binding to the virus-specific receptor proteins (11), which initiates virus ...a receptor of MHV (5). On the other hand, the receptor-binding domain on the S ... See full document
5
Selective host range restriction of goat cells for recombinant murine leukemia virus and feline leukemia virus type A.
... Based on host range, the murine leukemia virus MuLV system has the following four distinct, identifiable env genespecified subgroups: the ecotropic MuLV's, which can grow only in mouse a[r] ... See full document
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Characterization of Intracellular Ribonucleic Acid Specific for the Murine Sarcoma-Leukemia Virus Complex
... To determine whether cycloheximide treatment could result in accumulation of viral RNA within the 78A1 cells, experiments were first conducted to establish the effect of the inhibitor on[r] ... See full document
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Chimeric Human Immunodeficiency Virus Type 1 Containing Murine Leukemia Virus Matrix Assembles in Murine Cells
... 293 cells transfected with chimeric TE proviruses revealed viral particles in 5 to 10% of the cells ...mock-transfected cells. In cells transfected with the chimeric constructs, characteristic ... See full document
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