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[PDF] Top 20 Determinants in HIV 2 Env and tetherin required for functional interaction

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Determinants in HIV 2 Env and tetherin required for functional interaction

Determinants in HIV 2 Env and tetherin required for functional interaction

... infections HIV-2 stocks were produced in 293T cells by transient transfection using TurboFect and 10 μ g of proviral plas- mids, followed by harvesting and filtration of superna- tants 48 h ...a ... See full document

13

HIV 1 Vpu and HIV 2 Env counteract BST 2/tetherin by sequestration in a perinuclear compartment

HIV 1 Vpu and HIV 2 Env counteract BST 2/tetherin by sequestration in a perinuclear compartment

... retains tetherin in the TGN and simultaneously marks it for degradation by recruiting β-TrCP to mediate its ...its interaction with gp120 ...target tetherin [23-27,38,42] are consistent with the ... See full document

16

Antagonism of BST-2/Tetherin Is a Conserved Function of the Env Glycoprotein of Primary HIV-2 Isolates

Antagonism of BST-2/Tetherin Is a Conserved Function of the Env Glycoprotein of Primary HIV-2 Isolates

... primary HIV-2 isolates also exhibited Vpu-like activity, and viruses with Env proteins capable or incapable of antagonizing BST-2 were found to coexist in the same ...an Env isolate ... See full document

13

Separable Determinants of Subcellular Localization and Interaction Account for the Inability of Group O HIV-1 Vpu To Counteract Tetherin

Separable Determinants of Subcellular Localization and Interaction Account for the Inability of Group O HIV-1 Vpu To Counteract Tetherin

... efficient tetherin antagonism when combined with the M-Vpu TM ...requires interaction with the Vpu cytoplasmic tail and its phosphorylation, such putative conformational differ- ences do not affect this ... See full document

12

A Functional Interaction between gp41 and gp120 Is Observed for Monomeric but Not Oligomeric, Uncleaved HIV-1 Env gp140

A Functional Interaction between gp41 and gp120 Is Observed for Monomeric but Not Oligomeric, Uncleaved HIV-1 Env gp140

... Uncleaved HIV-1 Env gp140 Miklos Guttman, Kelly ...of HIV and the target for the humoral immune sys- ...gp140 Env constructs truncated at the transmembrane domain are being investigated ... See full document

14

Functional bottlenecks for generation of HIV 1 intersubtype Env recombinants

Functional bottlenecks for generation of HIV 1 intersubtype Env recombinants

... of HIV-1 intersub- type Env recombinants could be the key to understand- ing the labile C1/C5/ecto-gp41 interface and could help in design of drugs to disrupt this ...on HIV-1 env gene ... See full document

17

CCR5 interaction with HIV 1 Env contributes to Env induced depletion of CD4 T cells in vitro and in vivo

CCR5 interaction with HIV 1 Env contributes to Env induced depletion of CD4 T cells in vitro and in vivo

... of HIV-1 Env binding to CCR5 by overexpression of R5-tropic Env on cell surface or by using recombinant R5-tropic gp120 proteins [4, 5, ...R5-tropic Env has not been studied in HIV-1 ... See full document

13

Identification of Amino Acids in the Human Tetherin Transmembrane Domain Responsible for HIV-1 Vpu Interaction and Susceptibility

Identification of Amino Acids in the Human Tetherin Transmembrane Domain Responsible for HIV-1 Vpu Interaction and Susceptibility

... by HIV-1 Vpu to recognize ...other functional activities of tetherin, such as B cell differentiation, the inhibition of virus production, or the regu- lation of interferon release (6, 18, 24, ... See full document

14

Preadaptation of Simian Immunodeficiency Virus SIVsmm Facilitated Env-Mediated Counteraction of Human Tetherin by Human Immunodeficiency Virus Type 2

Preadaptation of Simian Immunodeficiency Virus SIVsmm Facilitated Env-Mediated Counteraction of Human Tetherin by Human Immunodeficiency Virus Type 2

... of HIV-2 H 12034, which was inactive in all assays, harbors a histidine at this ...of HIV-2, Schmitt and colleagues recently passaged an SIVsmm clone in humanized mice ...and env genes. ... See full document

24

Tetherin Antagonism by HIV-1 Group M Nef Proteins

Tetherin Antagonism by HIV-1 Group M Nef Proteins

... Nef required for tetherin an- ...human tetherin. Thus, the sequence determinants for the antago- nism of human tetherin appear to be complex and context depen- ... See full document

14

Complementation of diverse HIV 1 Env defects through cooperative subunit interactions: a general property of the functional trimer

Complementation of diverse HIV 1 Env defects through cooperative subunit interactions: a general property of the functional trimer

... the HIV-1 Env trimer, using analysis of functional complementation between coexpressed inactive variants harboring different functional ...of Env-mediated cell fusion, complementation ... See full document

11

Lack of adaptation to human tetherin in HIV 1 Group O and P

Lack of adaptation to human tetherin in HIV 1 Group O and P

... with tetherin, is predictive of anti-tetherin ...counteract tetherin, the lack of activity of the O26M chimera, which has a robust pre- sence in the TGN compartment, suggests that this is not ... See full document

18

HIV-1 Cell-Free and Cell-to-Cell Infections Are Differentially Regulated by Distinct Determinants in the Env gp41 Cytoplasmic Tail

HIV-1 Cell-Free and Cell-to-Cell Infections Are Differentially Regulated by Distinct Determinants in the Env gp41 Cytoplasmic Tail

... of HIV Env gp41 CT remain poorly understood despite being widely studied in the context of cell-free ...particle-associated Env or differential interaction with host or viral ...cell-to-cell ... See full document

14

The transmembrane domain of HIV 1 Vpu is sufficient to confer anti tetherin activity to SIVcpz and SIVgor Vpu proteins: cytoplasmic determinants of Vpu function

The transmembrane domain of HIV 1 Vpu is sufficient to confer anti tetherin activity to SIVcpz and SIVgor Vpu proteins: cytoplasmic determinants of Vpu function

... changes required for a gain of anti-tetherin activity of SIVcpz or SIVgor Vpus based on results obtained using M Vpus because the effect of sequence alterations is often context ...are required for ... See full document

14

Interaction between Reverse Transcriptase and Integrase Is Required for Reverse Transcription during HIV-1 Replication

Interaction between Reverse Transcriptase and Integrase Is Required for Reverse Transcription during HIV-1 Replication

... this interaction will lead to a defect specifically at the re- verse transcription ...the functional relevance of the putative RT-binding surface on the IN C-terminal domain, we introduced single amino acid ... See full document

12

HIV 1 Group P is unable to antagonize human tetherin by Vpu, Env or Nef

HIV 1 Group P is unable to antagonize human tetherin by Vpu, Env or Nef

... are required to exclude the possibility that they may counteract the tetherin restriction through an as yet unknown ...that HIV-1 Groups O and N can cause AIDS [45,46], whereas the pathogenic ... See full document

9

Upregulation of BST-2/Tetherin by HIV Infection In Vivo

Upregulation of BST-2/Tetherin by HIV Infection In Vivo

... including HIV, from escaping infected cells. The HIV protein Vpu antagonizes this host ...during HIV infection in vivo and whether it can be modulated to the host’s ...from HIV-infected ... See full document

11

The ESCRT-0 Component HRS is Required for HIV-1 Vpu-Mediated BST-2/Tetherin Down-Regulation

The ESCRT-0 Component HRS is Required for HIV-1 Vpu-Mediated BST-2/Tetherin Down-Regulation

... the HIV-ACE research network (HEALTH-F3-2008-201095) supported by a grant of the European Commission, within the Priority 1 Health work programme of the 7th Framework Programme of the ... See full document

20

Antibody-Mediated Enhancement of HIV-1 and HIV-2 Production from BST-2/Tetherin-Positive Cells

Antibody-Mediated Enhancement of HIV-1 and HIV-2 Production from BST-2/Tetherin-Positive Cells

... treatment of multiple myeloma and are considered for the treatment of solid tumors with high BST-2 antigen levels. Functionally, BST-2 restricts the secretion of retroviruses, including human ... See full document

14

HIV-2 as a model to identify a functional HIV cure

HIV-2 as a model to identify a functional HIV cure

... Two HIV virus types exist: HIV‑1 is pandemic and aggressive, whereas HIV2 is confined mainly to West Africa and less ...against HIV. Consequently, the concepts of functional ... See full document

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