[PDF] Top 20 Determination of viral proteins present in the human immunodeficiency virus type 1 preintegration complex.
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Determination of viral proteins present in the human immunodeficiency virus type 1 preintegration complex.
... Viral DNA can be purified from these extracts by gel filtration chromatography and sucrose gradient sedimentation as a part of a nucleoprotein complex containing integrase as the only vi[r] ... See full document
6
Human ESCRT-II Complex and Its Role in Human Immunodeficiency Virus Type 1 Release
... in virus budding must take into ac- count the observation that HIV-1 release is not significantly inhibited by depletion of endogenous CHMP5 and CHMP6 (reference 85 and this work), yet virus release ... See full document
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The Barrier-to-Autointegration Factor Is a Component of Functional Human Immunodeficiency Virus Type 1 Preintegration Complexes
... and viral proteins in fraction I would increase the level of cDNA recovery by MAb ...for 1 h at 4°C in a Centricon 100 concentrator (Millipore ...Whereas proteins with mo- lecular masses of ... See full document
7
A p6Pol-protease fusion protein is present in mature particles of human immunodeficiency virus type 1.
... fusion proteins were partially autoprocessed at the p6 Pol protein (see also ...FIG. 1. Immunoblot analysis of viral particles harvested from human cell culture ... See full document
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The C-Terminal Proline-Rich Tail of Human Immunodeficiency Virus Type 2 Vpx Is Necessary for Nuclear Localization of the Viral Preintegration Complex in Nondividing Cells
... munodeficiency virus SIVsm/SIVmac lineage encode ...other viral components, is a nu- clear protein ...accessory proteins mediate distinct func- tions during SIV infection ... See full document
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Reconstruction and Function of Ancestral Center-of-Tree Human Immunodeficiency Virus Type 1 Proteins
... of human immunodeficiency virus type 1 (HIV-1) and its capacity to mutate and escape host immune responses are major challenges for AIDS vaccine ...major viral structural ... See full document
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Potentiation of human immunodeficiency virus type 1 Tat by human cellular proteins.
... by human extracts, imply- ing that the required factor(s) is not present in these ...a complex between Tat and a host cell protein(s) or to covalent modification of ... See full document
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Interaction of the Human Immunodeficiency Virus Type 1 Vpr Protein with the Nuclear Pore Complex
... other viral proteins, is pre- sumed to be important for at least some of its ...this viral accessory protein are to be fully ...different proteins are present in multiple copies in each ... See full document
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Cytoskeletal proteins inside human immunodeficiency virus type 1 virions.
... a complex and poorly understood process, which most probably requires interactions between Gag and cellular proteins that might result in the incorporation of host proteins into the ...assembling ... See full document
10
Human immunodeficiency virus type 1 preintegration complexes containing discontinuous plus strands are competent to integrate in vitro.
... the viral DNA are integrated into a site in the right half of the same ...each viral DNA 3 9 end integrates into the strand of opposite polarity, yielding an internally inverted circle ...each viral ... See full document
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Nef association with human immunodeficiency virus type 1 virions and cleavage by the viral protease.
... not present in virions pro- duced by the DFCI-HT-PR 2 construct ...29-kDa proteins detected in viral particle preparations represented LAI Nef and ELI Nef, respectively (see ...auxiliary viral ... See full document
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Human immunodeficiency virus type 2 preintegration complexes: activities in vitro and response to inhibitors.
... (A) Proteins associated with HIV-2 viral cDNA. HIV-2 viral cDNA was immunoprecipitated and amplified by PCR to identify component ...with 1% Triton X-100 and incubated with 10 ml of polyclonal ... See full document
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Role of Human Immunodeficiency Virus Type 1 Integrase in Uncoating of the Viral Core
... the viral core. Mutational stud- ies of HIV-1 IN have demonstrated the pleiotropic effects of IN on different stages of the viral life cycle (10, 14, 17, 28, 44, 62, 64, 69), but to our knowledge, a ... See full document
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Mapping Viral DNA Specificity to the Central Region of Integrase by Using Functional Human Immunodeficiency Virus Type 1/Visna Virus Chimeric Proteins
... and complex protein-protein ...order complex (1, 3, 12, 17, 23, 26, 28, 29, 54, 73) and each active site might act only once (2, ...of viral DNA ends now seems ... See full document
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Evidence for a Functional Link between Uncoating of the Human Immunodeficiency Virus Type 1 Core and Nuclear Import of the Viral Preintegration Complex
... this virus in greater detail, we quantified early and late reverse transcripts synthesized during a 25-h time ...mutant virus produced levels of early RT products similar to wild type, and at similar ... See full document
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Role for Human Immunodeficiency Virus Type 1 Membrane Cholesterol in Viral Internalization
... of human immunodeficiency virus type 1 (HIV-1) virions contains high levels of cholesterol and sphingomyelin, an enrichment that is explained by the preferential budding of the ... See full document
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Human Immunodeficiency Virus Type 1 Vif Protein Is Packaged into the Nucleoprotein Complex through an Interaction with Viral Genomic RNA
... how virus-associated Vif could regulate viral ...to viral RNA and Gag, Vif has a critical role in stabilizing viral nucleoprotein ...with viral nucleoprotein complexes as well as the ... See full document
14
Hsp40 Facilitates Nuclear Import of the Human Immunodeficiency Virus Type 2 Vpx-Mediated Preintegration Complex
... is present in viral PICs and regulates their nuclear ...the viral core initiates within the periph- eral regions of the cell cytoplasm by association with the actin cytoskeleton after entry ...motor ... See full document
9
Lys-34, Dispensable for Integrase Catalysis, Is Required for Preintegration Complex Function and Human Immunodeficiency Virus Type 1 Replication
... the viral integrase (IN) acting upon the viral attachment (att) sites at the ends of linear viral cDNA within the context of a large nucleoprotein complex referred to as the ... See full document
8
The Vif and Gag proteins of human immunodeficiency virus type 1 colocalize in infected human T cells.
... was present in membrane-associated and soluble cytosolic ...(Fig. 1 and 3A); however, it is worth noting that these groups did not use nonpermissive T cells for their studies, nor did they examine ... See full document
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