[PDF] Top 20 Development of a New Chlamydiales Specific Real Time PCR and Its Application to Respiratory Clinical Samples
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Development of a New Chlamydiales Specific Real Time PCR and Its Application to Respiratory Clinical Samples
... the Chlamydiales order has extended considerably over the last several ...a new Chlamydiales-specific real-time PCR targeting the conserved 16S rRNA gene was devel- ... See full document
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Comparison of a New Quantitative ompA Based Real Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays
... the real-time PCR technology is an additional diagnostic tool that offers numer- ous advantages such as an automated, nonnested format in- cluding template inactivation and the combination of ... See full document
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A New Highly Sensitive and Specific Real Time PCR Assay Targeting the Malate Dehydrogenase Gene of Kingella kingae and Application to 201 Pediatric Clinical Specimens
... K ingella kingae is a significant pediatric pathogen responsible for bone and joint infections, occult bacteremia, and, more rarely, endocarditis that may occur either sporadically or in the context of outbreaks in ... See full document
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Comparison of Established Diagnostic Methodologies and a Novel Bacterial smpB Real Time PCR Assay for Specific Detection of Haemophilus influenzae Isolates Associated with Respiratory Tract Infections
... of respiratory tract infections (RTI) worldwide. The development of a rapid ...novel real-time PCR diagnostic assay targeting the smpB gene was designed to detect all serogroups of ... See full document
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Development of Conventional and Real Time PCR Assays for Detection of Legionella DNA in Respiratory Specimens
... The development and validation of a PCR assay based on the use of new 16S ribosomal DNA (rDNA)- targeted primers to detect Legionella DNA in respiratory specimens are ...conventional ... See full document
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Development and validation of Burkholderia pseudomallei-Specific real-time PCR assays for clinical, environmental or forensic detection applications
... Specificity of 122018, 266152, BurkDiff and TTS1 assays Following confirmation of assay accuracy, we screened DNA panels comprising 2,205 Burkholderia spp. (Table 2) and 127 non- Burkholderia species (Figure S1; Table ... See full document
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Development of a Real Time Reverse Transcription PCR for Detection of Newcastle Disease Virus RNA in Clinical Samples
... and specific method, it takes several days to make a routine ...reported. Real-time PCR and real-time reverse-tran- scription PCR (RRT-PCR) offer the high ... See full document
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Quantitative Detection of Moraxella catarrhalis in Nasopharyngeal Secretions by Real Time PCR
... the PCR assay for the detec- tion of ...on real-time (TaqMan) technology. Previously reported PCR as- says were either conventional end-point assays (7, 12, 32) or multiplex assays (15, ...to ... See full document
5
Development and Application of Real Time PCR Assay for Quantification of Mycobacterium ulcerans DNA
... the development of an IS2404-based real-time PCR assay for the quantification of ...conventional PCR assay (25) recommended in a slightly modified form (21) by WHO as a test for the ... See full document
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Specific and Sensitive Detection of Neisseria gonorrhoeae in Clinical Specimens by Real Time PCR
... the development of a specific and sensitive TaqMan assay for the detection of ...AMPLICOR-positive samples revealed that 68% were negative in both the 16S rRNA test and the opa assay (confirming that ... See full document
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Real time PCR using FRET technology for Old World cutaneous leishmaniasis species differentiation
... Leishmaniasis, an infection caused by obligate intrama- crophage protozoa transmitted predominantly by the bite of an infected phlebotomine female sandfly is en- demic throughout tropical and subtropical regions [1]. ... See full document
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Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
... the development of their primer sets, as well as 25 new sequences included in order to have at least one repre- sentative sequence for each species known to cause ... See full document
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Sensitive Detection of Mycoplasma pneumoniae in Human Respiratory Tract Samples by Optimized Real Time PCR Approach
... the real-time PCR, freshly grown cells of strain M129 were homogenized by using a 27G syringe and tenfold diluted in phosphate-buffered ...DNAs. Respiratory tract specimens (bronchoalveo- lar ... See full document
5
One step real time RT PCR assays for serotyping dengue virus in clinical samples
... A sample was classified as positive in the CDC DENV-1-4 real-time RT-PCR assays if the amplification curve crossed the threshold line within 37 cycles Five of the six DENV1 RT-PCR positi[r] ... See full document
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Detection of U S , Lelystad, and European Like Porcine Reproductive and Respiratory Syndrome Viruses and Relative Quantitation in Boar Semen and Serum Samples by Real Time PCR
... disrupting agent, such as dithiothreitol or -mercaptoethanol, so it is similar to those used in other differential lysis tech- niques that release only somatic cell DNA and not sperm cell DNA (22, 31, 35, 49). ... See full document
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Comparison of the GenMark Diagnostics eSensor Respiratory Viral Panel to Real Time PCR for Detection of Respiratory Viruses in Children
... six respiratory specimens are continuously processed and extracted throughout the day and evening shifts and tested with our 10-member respiratory virus panel of laboratory-developed real-time ... See full document
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Nasal swab samples and real time polymerase chain reaction assays in community based, longitudinal studies of respiratory viruses: the importance of sample integrity and quality control
... Between September 2010 and July 2012, 152 infants were recruited into the study. All participants lived within the greater Brisbane metropolitan area and none were from rural communities. One-hundred and twenty- five ... See full document
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Multicenter, Prospective Clinical Evaluation of Respiratory Samples from Subjects at Risk for Pneumocystis jirovecii Infection by Use of a Commercial Real Time PCR Assay
... itative real-time PCR test utilizing molecular beacons (36) for the detection of ...SmartCycler real-time PCR system was used to monitor the fluorescence emitted by each beacon ... See full document
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Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... the ITS 2 region (30) have been ...broad-range real-time PCR assay tar- geting clinical relevant fungal pathogens besides Candida and Aspergillus in one assay is currently not ...the ... See full document
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Simultaneous Detection of Pathogens in Clinical Samples from Patients with Community Acquired Pneumonia by Real Time PCR with Pathogen Specific Molecular Beacon Probes
... The target genes for the six pathogens were selected for the following reasons. The lytA gene encoding autolysin enzyme as well as the pneumolysin gene are specific to S. pneumoniae (26, 29); therefore, the lytA ... See full document
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