[PDF] Top 20 Development of a DNA probe to detect Salmonella typhi
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Development of a DNA probe to detect Salmonella typhi
... Using various enteric bacterial strains, we tested probe specificity with nitrocellulose and Whatman 541 paper and found Whatman 541 paper to have several advantages when used in the col[r] ... See full document
6
Molecular typing of Salmonella typhi strains from Dhaka (Bangladesh) and development of DNA probes identifying plasmid encoded multidrug resistant isolates
... plasmid DNA present in the Dhaka MDR strains (R types CATmSSuT and ATmSSuT) and the MDR strain from Paki- stan (R type ...recognize DNA sequences that are present in the large plasmids of the H1 ... See full document
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Identification of Salmonella enterica Serovar Typhi Genotypes by Use of Rapid Multiplex Ligation Dependent Probe Amplification
... plasmid probe, a bias toward isolates from Vietnam, and the fact that some indel profiles are shared by several genetically diverse ...S. Typhi genotypes, while other indel profiles define much more ... See full document
9
A novel method of selective removal of human DNA improves PCR sensitivity for detection of Salmonella Typhi in blood samples
... human DNA: MolYsis (Molzym GmbH & ...eukaryotic DNA was subsequently degraded by the addition of a chaotropic resistant nuclease, and the intact bacterial cells were collected for bacterial DNA ... See full document
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... different Salmonella serotypes with different antibiotic sensitivity is present then use of single antibiotic may eradicate one serotype while the other if resistant to that drug may ...specific DNA ... See full document
6
Application of salmonella typhi’s outer membrane (omp) in diagnosis of typhoid
... S. typhi from ...S. typhi that have been developed for the diagnosis of typhoid fever, the most widely used is the Widal test, which is an inexpensive and simple method (Appassakij et ...for ... See full document
6
DETECTION OF SALMONELLA TYPHI AND HELICOBACTER GENUS IN GALLSTONE PATIENTS
... pylori DNA in biliary tissue and gallstones, however there are several problems with these studies that some samples were collected at endoscopic retrograde cholangiopancreatography(Silvaet ... See full document
16
The Cytolethal Distending Toxin Produced by Nontyphoidal Salmonella Serotypes Javiana, Montevideo, Oranienburg, and Mississippi Induces DNA Damage in a Manner Similar to That of Serotype Typhi
... nontyphoidal Salmonella (NTS) serotypes represent some of the most common causes of food-borne illnesses world- wide and therefore have a tremendous economic and public health ...S. Typhi have suggested ... See full document
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A non-spectroscopic optical biosensor for the detection of pathogenic Salmonella Typhimurium based on a stem-loop DNA probe and retro-reflective signaling
... Culture-based methods are considered to be standard for the diagnosis of Salmonella. However, they are not widely used due to their time-consuming nature. Other disadvantages of these methods include costly and ... See full document
8
Molecular Determination of H Antigens of Salmonella by Use of a Microsphere Based Liquid Array
... not detect all flagellar anti- gen ...for Salmonella, and dramatically increase the speed for complete serotyping, as phase reversal would no longer be ... See full document
9
Drug-resistant enteric fever worldwide, 1990 to 2018: a systematic review and meta-analysis
... Typhoid-specific lower breakpoints against fluoroqui- nolones (FQ) came into effect during our study period [24]. To allow the analysis of resistance trends over time, we classified ciprofloxacin intermediate (minimum ... See full document
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... in Salmonella for the former first-line ACCoT (ampicillin, chloramphenicol, cotrimoxazole and tetracycline) antibiotics in the late 1980s led to the use of fluoroquinolones for ...that Salmonella strains ... See full document
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Epidemiology, Clinical Presentation, Laboratory Diagnosis, Antimicrobial Resistance, and Antimicrobial Management of Invasive Salmonella Infections
... 50-kDa Salmonella serovar Typhi outer membrane pro- tein (OMP) in an immunodot test ...The Typhi- Rapid IgM and TyphiRapid IgG IgM (Combo) are similar ver- sions of the test but in an ICT cassette ... See full document
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Rapid Emergence of Multidrug Resistant, H58-Lineage Salmonella Typhi in Blantyre, Malawi
... Typhoid fever is a major cause of disease and death around the world, particularly in re- source limited settings, although reports suggest that until recently it has been much less prominent in sub-Saharan Africa (SSA) ... See full document
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Towards HIV Detection: Novel Poly(propylene imine) Dendrimer-Streptavidin Platform for Electrochemical DNA and gp120 Aptamer Biosensors
... The biosensor was prepared by the immobilisation of single stranded 5'- biotynilated probe DNA and B40 aptamer on the modified electrode and then used to detect complementary DNA and gp1[r] ... See full document
13
Typhoid Fever and Liver Enzyme Activity
... incubation period for typhoid is longer than that for salmonellosis, usually one week. Historical accounts of typhoid written before the development of antimicrobial chemotherapy emphasized four main stages, ... See full document
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ANTIBIOGRAM OF SALMONELLA TYPHI AND SALMONELLA PARATYPHI A IN A TERTIARY CARE HOSPITAL
... Enteric fever in India is commonly caused by S.Typhi and S. Paratyphi A, with a ratio of 10:1 cases, respectively. [14] In recent years there is an increase in isolation of S. Paratyphi A. [15, 16] In the present study ... See full document
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Detection of murine typhus infection in fleas by using the polymerase chain reaction
... Polymerase chain reaction PCR amplification of DNA was used to detect the etiologic agent of murine typhus, Rickettsia typhi, in experimentally infected adult fleas.. A primer pair deriv[r] ... See full document
5
Early Infection of Bilateral Total Knee Arthroplasty with Salmonella typhi : Case Report and Review of Literature
... S typhi. In previously reported cases with Salmonella 50 percent had an underlying immunosuppressive condition [7,15]- Sa typhi is mainly acquired through orofecal contamination with Gastrointestinal ... See full document
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Clinical outcomes in typhoid fever: adverse impact of infection with nalidixic acid resistant Salmonella typhi
... of Salmonella enterica serotype typhi (MDR-ST) that were resistant to all the three first-line drugs then in use, namely chloramphenicol, amoxycillin and co-trimoxa- zole emerged, and sooner MDR-ST became ... See full document
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