[PDF] Top 20 Development of a new duplex real time polymerase chain reaction assay for hepatitis B viral DNA detection
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Development of a new duplex real time polymerase chain reaction assay for hepatitis B viral DNA detection
... active viral replication is detec- tion of HBV DNA in plasma or serum ...on real-time PCR have been developed for quantification of HBV DNA in serum or plasma samples ...tests, ... See full document
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Original Article Novel real-time polymerase chain reaction assay for quantitative detection of Chlamydia trachomatis in vaginal and cervical secretions
... traditional detection methods, this newly developed real-time PCR method is more sensitive and specific, espe- cially in terms of quantitative detection of ...samples, real-time ... See full document
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Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... H5 subtype AIVs can be classified into two groups based on their pathogenicity on chickens: high patho- genicity and low pathogenicity groups [6]. Highly patho- genic H5 subtype AIVs have infected wild birds, and ... See full document
7
PCR and antibody methods: Research compares two cattle feed tests that detect bovine byproduct contaminants
... under development for FDA ...these detection methods, evaluating their ability to detect ruminant contami- nation in cattle ...a real-time polymerase chain reaction ... See full document
5
Development of a panel of seven duplex real-time PCR assays for detecting 13 streptococcal superantigens
... The reaction conditions of the duplex real-time PCR were optimized and the specificity of the duplex assays was evaluated using SAg positive ...of detection of the duplex ... See full document
5
Development of a fluorescent quantitative real time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
... homogenization. DNA was extracted from the tissue samples by using the method described by Cheng ...this assay, we could quantify the viral ...The viral concentra- tions were expressed as the ... See full document
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New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... a new detection platform ...Taq DNA polymerase based reaction components, with a HDA amplification cocktail, which is now commercially available in various formats from Biohelix Corp, ... See full document
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A novel duplex real time reverse transcriptase polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control
... fluorescence detection kits), the duplex primer/probe assay has many ...fluorescence detection kit required 900-μl of serum for HCV RNA ...the duplex primer/probe assay barely ... See full document
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Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... and real-time PCR, and detection based on DNA or ...PCR development has been done for food samples from non-fishery ...products. Real time PCR is a more recently developed ... See full document
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A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification
... the detection of Norovirus and bovine viral diarrhoea virus ...were real-time PCR ...RT-PCR assay without RNA extraction with less than 1% serum ...dRT-PCR assay remains func- ... See full document
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Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... the detection of adenovirus in these cases. Importantly, the assay demonstrated the detection of dual Adeno and CMV and Adeno and RSV in clinical samples, underscoring the importance of complete ... See full document
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Real-Time Polymerase Chain Reaction for the Rapid Detection of Group B Streptococcal Colonization in Neonates
... the time of delivery are currently observed in the hospital for ⱖ 48 hours and evaluated and perhaps treated for sepsis in the presence of clinical signs or maternal risk ...of real-time PCR, which ... See full document
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Detection of hepatitis B virus DNA in serum by polymerase chain reaction amplification and microtiter sandwich hybridization
... The DNAs from 69 hepatitis B surface antigen-positive serum samples and 16 antigen-negative control samples were amplified by the PCR procedure, and the product was detected by Southern [r] ... See full document
6
Clustering techniques for DNA computing readout method based on real-time polymerase chain reaction
... In 1936, Alan Turing designed the Turing Machine [12], a rule-based device that moves over a limitless tape with symbols written on it and can read, write, and rewrite these symbols. The Turing machine marks the ... See full document
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Specific detection of Campylobacter jejuni and Campylobacter coli by using polymerase chain reaction
... Development of a routine detection assay for Campylobacter jejuni and Campylobacter coli in clinical specimens was undertaken by using the polymerase chain reaction PCR.. An oligonucleot[r] ... See full document
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Diagnostic Accuracy of In House PCR for Pulmonary Tuberculosis in Smear Positive Patients: Meta Analysis and Metaregression
... In conclusion, contrary to the increasing importance of NAATs for differential diagnosis of patients with suspected mycobacterial infection, this meta-analysis demonstrated that the scientific background for the use of ... See full document
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Development of a Quantitative Real Time Detection Assay for Hepatitis B Virus DNA and Comparison with Two Commercial Assays
... MONITOR assay within the lower de- tection range, which can even be more than 100%, is the main disadvantage of this assay ...The real-time PCR detection assay described here had ... See full document
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Pathways to false positive diagnoses using molecular genetic detection methods; Phytophthora cinnamomi a case study
... geographic location and only tested against species thought to occur within that environmental system. For example, Engelbrecht et al. (2013) designed primers for use in detecting P. cinnamomi in the avocado industry in ... See full document
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Comparison Between Real-Time Polymerase Chain Reaction and DNA-Microarray in Detection and Identification of Mycobacterium Species
... The LCD array analysis showed that the 80 positive cultures from urine specimens contained six species of pathogenic mycobacterial isolates. The LCD array assay showed different patterns according to the ... See full document
9
Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran
... have been developed with the capability of detecting FMDV in a variety of sample matrices (15, 16). These assays characteristically possess both high analytical sensitivity and high specificity. The ability to detect ... See full document
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