[PDF] Top 20 Development and Evaluation of Real Time PCR Based Fluorescence Assays for Detection of Chlamydia pneumoniae
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Development and Evaluation of Real Time PCR Based Fluorescence Assays for Detection of Chlamydia pneumoniae
... Chlamydia pneumoniae is an important respiratory pathogen recently associated with atherosclerosis and several other chronic ...diseases. Detection of C. pneumoniae is inconsistent, and ... See full document
9
Real-Time PCR Detection of Mycoplasma pneumoniae, Chlamydia pneumoniae and Legionella pneumophila in Respiratory Specimens Using the ARIES® System
... assay development, which is extremely valuable to smaller clinical laboratories with limited ...open PCR platform with the capacity to design primers for multiple targets as well as internal controls, this ... See full document
6
The Pneumoplex Assays, a Multiplex PCR Enzyme Hybridization Assay That Allows Simultaneous Detection of Five Organisms, Mycoplasma pneumoniae, Chlamydia (Chlamydophila) pneumoniae, Legionella pneumophila, Legionella micdadei, and Bordetella pertussis, and Its Real Time Counterpart
... in detection and diag- ...These assays are based on amplification of either isolated nucleic acid or whole-organism DNA by PCR with gene-specific ...singleplex PCR assays have ... See full document
7
Comparison of an in house real time duplex PCR assay with commercial HOLOGIC® APTIMA assays for the detection of Neisseria gonorrhoeae and Chlamydia trachomatis in urine and extra genital specimens
... duplex PCR assay ...the time of PCR testing, the duplex PCR assay did not include a target for an internal human DNA control in order to validate the quality of the extracted ...in-house ... See full document
7
Comparison and Evaluation of Real Time PCR, Real Time Nucleic Acid Sequence Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae
... Mycoplasma pneumoniae is a common cause of community-acquired pneumonia and lower-respiratory-tract ...these assays is limited. The development of a novel duplex real-time PCR ... See full document
6
Evaluation of Multiplex Type Specific Real Time PCR Assays Using the LightCycler and Joint Biological Agent Identification and Diagnostic System Platforms for Detection and Quantitation of Adult Human Respiratory Adenoviruses
... conventional PCR for HAdV-B3, -E4, -B7, -B11, -B14, or ...Chlamydophila pneumoniae (ATCC 53592), Escherichia coli, Klebsiella pneu- moniae (ATCC 13883), Pseudomonas aeruginosa (ATCC 97), Mycoplasma pneu- ... See full document
7
Development of Real Time PCR Assays and Evaluation of Their Potential Use for Rapid Detection of Burkholderia pseudomallei in Clinical Blood Specimens
... various PCR-based detection methods, in both con- ventional end-point and real-time formats, for specific and rapid identification of the bacterium for earlier diagnosis of melioidosis ... See full document
8
Development of a Real Time Fluorescence PCR Assay for Rapid Detection of the Diphtheria Toxin Gene
... culture detection. Consequently, molecular detection of the diphtheria toxin gene, tox, is often the only means by which a laboratory con- firmation of diphtheria can be ...standard PCR assay that ... See full document
7
Comparison of a New Quantitative ompA Based Real Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays
... five assays detected purified C. pneumoniae DNA down to very low levels and detected DNA in the mock-infected sputum, and depending on the target level, the results could be reproduced in most ...from ... See full document
9
Development of Real Time Multiplex Nucleic Acid Sequence Based Amplification for Detection of Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella spp in Respiratory Specimens
... multiplex assays has rarely been performed (6, 7, 9, 10, 27, ...a real-time multiplex NASBA than of the mono NASBAs for the quantification of human cytomegalovirus IE1 mRNA ...M. pneumoniae in ... See full document
7
Development and Evaluation of an ompA Quantitative Real Time PCR Assay for Chlamydia trachomatis Serovar Determination
... qPCR assays for clinical ...ET Chlamydia trachomatis assay (Becton Dickinson, Sparks, MD) or the COBAS Amplicor analyzer (Roche ...evaluate detection of the A to C ... See full document
6
Molecular Diagnosis of Chlamydia pneumoniae Infection
... in PCR buffers, use of proteins for enzyme stabili- zation, and alteration of annealing, extension, and denatur- ation temperatures and times and cycle number (34, 67, ...touchdown PCR (19) with decreasing ... See full document
9
Development of Conventional and Real Time PCR Assays for Detection of Legionella DNA in Respiratory Specimens
... diagnosis based on successful culturing of Legionella organisms ...evaluated assays to detect Legionella DNA in respiratory tract specimens and sometimes also in serum or urine (1, 6–8, 9–12, 14–16, ...23). ... See full document
7
Real Time PCR Assays for Detection of Bocavirus in Human Specimens
... Clinical specimens. Respiratory specimens were collected from 1,178 hospi- talized pneumonia patients identified through active population-based surveil- lance for pneumonia in Sa Kaeo Province, Thailand, between ... See full document
5
Eggert, Marlene (2011): Nachweis und Charakterisierung von Shigatoxin-bildenden Escherichia coli in Wildwiederkäuern. Dissertation, LMU München: Tierärztliche Fakultät
... using real-time PCR and culturing ...The real-time PCR screening was based on the stx1 and stx2 ...the detection of eae, hlyA and saa and characterized ...duplex ... See full document
150
Multicenter Comparison Trial of DNA Extraction Methods and PCR Assays for Detection of Chlamydia pneumoniae in Endarterectomy Specimens
... overall detection rate (9%) and the maximum concor- dant positive rate (25% for one carotid artery) for the ather- oma samples were lower than ...of PCR inhibitors within clinical sam- ...of PCR ... See full document
6
Development and Validation of a Quantitative Real Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis
... nested PCR approach as a method to increase the sensitivity of the assay because of the major difficulties of amplicon contamination inherent in such a method (10, ...nested PCR format also undermines the ... See full document
7
SIMULTANEOUS DETECTION OF CHLAMYDIA PNEUlvlONIAE AND MYCOPLASMA PNEUMONIAE BY PCR
... Respiratory tract infections are important burdens on the health service, and pneumonia is probably the most common serious infection seen in general practice. Our understanding of the etiological agents of lower ... See full document
6
Analytical Sensitivity, Reproducibility of Results, and Clinical Performance of Five PCR Assays for Detecting Chlamydia pneumoniae DNA in Peripheral Blood Mononuclear Cells
... C. pneumoniae DNA by one PCR assay while 2 were positive by a second PCR assay and 3 other assays de- tected no ...of PCR assays, as we have shown in the present ...the ... See full document
6
Rapid Detection of Chlamydia pneumoniae by PCR Enzyme Immunoassay
... for detection of C. pneumoniae (7, ...C. pneumoniae are the omp1 gene and a specific DNA fragment (5, ...Various PCR procedures for detection of C. pneumoniae by different detec- ... See full document
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