[PDF] Top 20 Development and Evaluation of a Loop Mediated Isothermal Amplification Method for Rapid Detection of Aspergillus fumigatus

... qPCR detection on clinical samples showed that they had good concordance; besides simplicity, however, the detection limit and speed of the LAMP assay seemed better than those of qPCR for clinical ... See full document

... threshold value of ⱖ0.1 was considered positive. In addition, 10-␮l aliquots of RT-LAMP products were electrophoresed on a 3% NuSieve 3:1 agarose gel (BMA, Rockland, Maine) for electrophoresis in Tris-borate buffer, ... See full document

... A LAMP amplification kit (Eiken Chemical Co. Ltd., Tokyo, Japan) was used for the LAMP reaction. The re- action was prepared according to the manufacturer’s instructions in a total volume of 25 μL. The final ... See full document

... purposes, development of rapid, economic and effective on-site detection methods for GM components is ...GMO detection are based on polymerase chain reaction (PCR). PCR method needs ... See full document

... years, isothermal amplifications, espe- cially loop-mediated isothermal amplification (LAMP) [12] has revolutionized the field of molecular diagnostics by offering rapid and ... See full document

... minimum detection limit of the real-time RT-LAMP assay was ...this method presented a similar sensitivity with real-time RT-PCR and was somewhat sensitive than one-step RT-PCR in detection of ... See full document

... Human African trypanosomiasis is endemic in tropical Africa. In eastern and southern Africa the disease is caused by Trypanosoma brucei rhodesiense, while T. b. gambiense infections are common in central and West Africa. ... See full document

... 15]. Rapid detection is very important for the prevention and control of ...the detection of the H5, H7 and H9 subtypes of AIVs have been devel- oped and applied to surveillance and clinical care ... See full document

... PCR method are completely avoided ...easier detection by visual inspection, by observation of either an increase in turbidity caused by gener- ation of magnesium pyrophosphate or a color change after ... See full document

... electrophoresis. Amplification was completed in PCR microtubes in an electric thermostatic water bath (Suopu, Shanghai, China) at the isothermal condition of 60°C for 60 ... See full document

... equipment. Loop mediated isothermal amplification technique (LAMP) is a technique developed by Notomi et ...under isothermal conditions. This nucleic acid amplification technique ... See full document

... to detection of other key genetic traits such as fungicide resistance, one recent example being an assay targeted to a 34 bp tandem repeat in the cyp51A gene that has been associated with azole resistance in ... See full document

... Based on 40 clinical samples from suspected HFMD cases, 92.9% diagnostic sensitivity was achieved with the RT-LAMP assay. Two samples tested negative by the RT-LAMP assay but positive by the rRT-PCR assay. The ... See full document

... stem- loop DNAs with various sizes of stem and cauliflower-like structures having multiple loops, which is induced by alternately annealing inverted repeats of the target sequence in the same strand ...LAMP ... See full document

... RNA amplification kit and a fluorescent detec- tion reagent (Eiken Chemical ...the detection of EV71 and ...the detection of EV71 and CVA16 was conducted using a total of 515 clinical ... See full document

... Theoretically, Bst polymerase used in LAMP has a higher tolerance of inhibitors than Taq polymerase used in qPCR [63, 64]. This suggests that the sensitivity of LAMP is slightly higher than that of qPCR, which is use- ... See full document

... via amplification of genetic material (DNA or RNA) by PCR has become a “gold standard” for rapid diagnosis of viral diseases, including ...for rapid diagnosis of CaPVs, including conven- tional and ... See full document

... standard method- for isolation of bacterium from stool specimens, for which Cefsulodin-Irgasan-Novobiocin agar (CIN) is the selective ...upon amplification of DNA or RNA and do not require high microbial ... See full document

... A total of 81 tissue samples were collected from chick- ens suspected of ALV infection, which had died from hemangioma or dramatically weight loss. These samples were detected by the LAMP assay and by PCR and virus ... See full document

... Loop-mediated isothermal amplification (LAMP), a novel nucleic acid amplification method, was developed for the clinical detection of four species of human malaria ... See full document