[PDF] Top 20 The development of a rapid SYBR Green I based quantitative PCR for detection of Duck circovirus
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The development of a rapid SYBR Green I based quantitative PCR for detection of Duck circovirus
... Real-time PCR was done using the Mastercycler ® ep real- plex system (Eppendorf, Germany) with SYBR Green I detection and Tm ...analysis. SYBR ® Premix Ex Taq™ (per- fect real ... See full document
6
Development of Group and Serotype Specific One Step SYBR Green I Based Real Time Reverse Transcription PCR Assay for Dengue Virus
... one-step SYBR Green-based RT-PCR could re- place the conventional cell culture method as the new gold standard in future clinical diagnosis of dengue virus ...of PCR in molecular ... See full document
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Establishment of one step SYBR green based real time PCR assay for rapid detection and quantification of chikungunya virus infection
... the SYBR green I-based RT-PCR for the quantitation and detection of CHIKV is ...decreases quantitative reliability. The SYBR Green-based RT-PCR ... See full document
7
Development of a SYBR green I based RT PCR assay for yellow fever virus: application in assessment of YFV infection in Aedes aegypti
... one-step SYBR Green I-based real-time quantitative RT-PCR (qRT-PCR) assay targeting the 5 ’ NTR and capsid-gene junction – for rapid detection and ... See full document
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Direct Detection of Emergent Fungal Pathogen Candida auris in Clinical Skin Swabs by SYBR Green Based Quantitative PCR Assay
... auris SYBR green qPCR performed well in comparison with the established culture method, indicating that it could be an effective rapid alternative to culture for the identification of ...auris ... See full document
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Simultaneous detection of porcine pseudorabies virus, porcine parvovirus and porcine circovirus type 2 by multiplex real-time PCR and amplicon melting curve analysis using SYBR Green I
... porcine circovirus type 2 can cause reproductive failure in pigs, and swine are often simultaneously infected by combinations of the three ...the development of a SYBR Green ... See full document
9
Development and Application of a Universal Hemoplasma Screening Assay Based on the SYBR Green PCR Principle
... universal SYBR green PCR assay described here was designed as a quantitative, closed-tube method for inexpensive and rapid screening of samples for hemotropic Mycoplasma ...hemoplasma ... See full document
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SYBR Green Based Real Time Quantitative PCR Assay for Detection of West Nile Virus Circumvents False Negative Results Due to Strain Variability
... Real-time quantitative PCR is used routinely for the high-throughput diagnosis of viral pathogens, such as West Nile virus ...dye SYBR green. The SYBR green-based assay ... See full document
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Development of a duplex SYBR Green I based quantitative real time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses
... the PCR cycles, a melting curve was generated (with the annealing temperatures 60 °C to 95 °C at a linear transition rate of ...duplex quantitative PCR were conducted using single or double standard ... See full document
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Detection of Duck Hepatitis B Virus DNA on Filter Paper by PCR and SYBR Green Dye Based Quantitative PCR
... in quantitative PCR, because SYBR green dye can bind to any double- stranded DNA, even nonspecific ...by PCR product, because illegitimate amplifications that incor- porate dUTP can be ... See full document
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TANDEM RECOMBINANT PLASMID CONSTRUCTION AS POSITIVE CONTROL FOR PIK3CA H1047R DETECTION BASED ON SYBR GREEN I qPCR
... the detection of both wild-type and mutant PIK3CA ...Efficient detection system can be obtained by using this tandem positive control in genotyping qPCR since it can shorten operational time and cost of the ... See full document
8
Molecular detection of human rhinoviruses in respiratory samples: a comparison of Taqman probe , SYBR green I and BOXTO based real time PCR assays
... [41]. Detection of HRV by culture is slow and complex for HRVA and HRVB, whilst HRVC has been unculturable in vitro to date ...and rapid antigen test kits are not available ...RT- PCR appear to be ... See full document
7
Duplex Real Time SYBR Green PCR Assays for Detection of 17 Species of Food or Waterborne Pathogens in Stools
... the detection of the following gastrointestinal infec- tious organisms: Shigella ...for detection of EPEC and EHEC was done within 1 ...conventional PCR assay was done using seven primer sets for the ... See full document
13
Real time PCR for the rapid identification and drug susceptibility of Mycobacteria present in Bronchial washings
... Of the 150 patients included in the study, 93 were males. The men: women ratio was 1.6:1. The age range was 12 to 83 years. 32 % of the males were above the age of 60 years while in females only 11 % were above the age ... See full document
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Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis
... primers based on the unique, repetitive Neospora-specific Nc5 sequence which has not been detected in other taxa [7, ...the detection of ...a detection limit equivalent to one ... See full document
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Bax Inhibitor-1 down-regulation in the progression of chronic liver diseases
... Real-time PCR was conducted in an ABI 7900 Sequence Detection System (Applied Biosystems, Foster City, CA, USA) using SYBR Green I ...(SYBR Green Master Mix), 300 nM of ... See full document
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Homology Modelling and Structural Comparisons of Capsid Associated Proteins from Circoviruses Reveal Important Virus Specific Surface Antigens
... In 2011 [18] the crystal structure of PCV2 Cap was determined. To crystallize PCV2 Cap, 40 amino acids were omitted from the N-terminal, which contains the NLS, however, the structure placed the NLS inside the capsid ... See full document
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Rapid detection of porcine circovirus type 2 using a TaqMan based real time PCR
... real-time PCR system, which amplified a 149-bp fragment to detect ...time PCR approach increased the detection of PCV2 samples by 18% over that achieved by conventional ...real-time PCR system ... See full document
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Comparison of Real Time PCR Assays for Detection of Pathogenic Leptospira spp in Blood and Identification of Variations in Target Sequences
... real-time PCR assays have been described, and these assays are now used in many diagnostic and reference laboratories for the detection of leptospires in biological fluids of ...patients. PCR of ... See full document
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Potentials and limitations of molecular diagnostic methods in food safety
... In many cases the genomic DNA of the host organism itself, especially from ground or processed meat or from complex food products, can interfere with the detection procedure by competing for the probe and primer ... See full document
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