[PDF] Top 20 Development of a Real Time PCR Approach for the Specific Detection of Prunus mahaleb
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Development of a Real Time PCR Approach for the Specific Detection of Prunus mahaleb
... the Prunus species present, because of significant cross-reactivity with other species within the Prunus ...novel real-time PCR assay was developed for the specific ... See full document
9
Development of a Novel Genus Specific Real Time PCR Assay for Detection and Differentiation of Bartonella Species and Genotypes
... novel real-time PCR assay for detection of Bartonella ...this real-time PCR assay in combination with se- quencing, we successfully amplified Bartonella DNA from rumi- ... See full document
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Development and validation of Burkholderia pseudomallei-Specific real-time PCR assays for clinical, environmental or forensic detection applications
... Specificity of 122018, 266152, BurkDiff and TTS1 assays Following confirmation of assay accuracy, we screened DNA panels comprising 2,205 Burkholderia spp. (Table 2) and 127 non- Burkholderia species (Figure S1; Table ... See full document
9
Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis
... The diagnosis of canine neosporosis is based on com- bining appropriate clinical signs and serology such as immunofluorescent antibody tests and enzyme-linked immunosorbent assays (ELISAs) [6]. However, these assays may ... See full document
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Development of real time PCR for detection and quantitation of Dengue Viruses
... Three hundred and eight blood samples were used to eval- uate the usefulness of the qRT-PCR assay for its diagnostic potential. The blood samples were obtained within 10 days post onset of symptoms. The sera were ... See full document
8
Specific and Sensitive Detection of Neisseria gonorrhoeae in Clinical Specimens by Real Time PCR
... the development of a specific and sensitive TaqMan assay for the detection of ...lower detection limit of one genome per reaction, the opa test appeared more sensitive than both the COBAS ... See full document
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Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... women. Specific and sensitive PCR methods are needed for diagnosis of this bacterium because it is very difficult to culture from patient ...quantitative real-time LightCycler PCR was ... See full document
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Development and Evaluation of a Genus Specific, Probe Based, Internal Process Controlled Real Time PCR Assay for Sensitive and Specific Detection of Blastocystis spp
... A previous comparison between XIVC and conventional PCR (amplifying 550 bp) revealed a nonsignificant difference in sensi- tivity in favor of PCR (30). Although the sensitivity of the TaqMan assay is higher ... See full document
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Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
... and specific The 18S rRNA gene is commonly used for developing primers for both detection and sequencing of protists because of its high conservation across the target genus, and its regions of variation ... See full document
8
Original Article Development of TaqMan real-time PCR for detection of stenotrophomonas maltophilia
... TaqMan real-time PCR was established to detect ...titative detection of clinical samples. In addi- tion, the TaqMan real-time PCR of ... See full document
8
A novel real time PCR assay for specific detection of Brucella melitensis
... tool, real-time PCR approaches provide a much faster ...species-specific real-time PCR for the detection of all biovars of Brucella melitensis, which could be used ... See full document
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Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... the real time RT-PCR detec- tion method for H5N6 subtype AIV to prevent and con- trol the spread of H5N6 ...fast, specific, and sensitive TaqMan real time RT-PCR method ... See full document
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Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
... melt-curve approach could more accurately identify the small difference in T M between these three species an assay based on HRM was ...sive real-time PCR machine ... See full document
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Improved Safety for Molecular Diagnosis of Classical Rabies Viruses by Use of a TaqMan Real Time Reverse Transcription PCR “Double Check” Strategy
... the real-time RT-PCR technol- ogy is the minimizing of cross-contamination based on the amplicon detection without opening the lid of the reaction ...tag- specific fluorophore be ... See full document
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Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... and specific diagnostic assays, fully validated for the detection of H5 viruses belonging to all hemagglutinin (HA) clades, particularly the recently circulating H5N1 viruses of clade ...the ... See full document
9
Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... Real-time PCR is a powerful tool for detection of human patho- gens because of the high sensitivity and throughput ...enables development of a multiplex platform for amplifica- tion and ... See full document
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<p>Detection of <em>Mycoplasma pneumoniae</em> in Mexican children with community-acquired pneumonia: experience in a tertiary care hospital</p>
... The detection of M. pneumoniae in respiratory samples was carried out by real-time PCR amplifying two geno- mic targets (p1 and CARDS) with primers and probes described ... See full document
11
Evaluation of Multiplex Type Specific Real Time PCR Assays Using the LightCycler and Joint Biological Agent Identification and Diagnostic System Platforms for Detection and Quantitation of Adult Human Respiratory Adenoviruses
... Specimen processing. Throat swabs and cultured isolates were processed in a biosafety level 2 hood. Nucleic acid was extracted from 175-l aliquots using the MagNA Pure LC DNA isolation kit I (Roche, Indianapolis, IN), ... See full document
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High occurrence of Blastocystis sp subtypes 1–3 and Giardia intestinalis assemblage B among patients in Zanzibar, Tanzania
... genus specific primer pair Ehd-239 F and Ehd-88R and the species specific probes Histolytica 96 T and Dis- par 96 T ...Universal PCR Master Mix (Applied Biosys- tems, Life Technologies, Stockholm, ... See full document
12
Development of an optimized method for the detection of airborne viruses with real time PCR analysis
... years, including liquid impingers, solid impactors, filters, electrostatic precipitators, and many others [8]. In this study, we used low melting agarose (LMA) as a capture medium instead of agar based media, since ... See full document
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