[PDF] Top 20 Development and Validation of a Real Time PCR Assay for Rapid Detection of Candida auris from Surveillance Samples
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Development and Validation of a Real Time PCR Assay for Rapid Detection of Candida auris from Surveillance Samples
... C. auris isolate M5658, a major genotype belonging to the South Asia clade and involved in the current outbreak in New York, was used to assess the analytical sensitivity of the real-time PCR ... See full document
7
Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... broad-range real-time PCR was developed for the rapid detection of human pathogenic ...The assay targets a part of the 28S large-subunit ribosomal RNA (rDNA) ...Aspergillus, ... See full document
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Development and Validation of a Quantitative Real Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis
... of assay format, primers, and probes warrant ...nested PCR approach as a method to increase the sensitivity of the assay because of the major difficulties of amplicon contamination inherent in such a ... See full document
7
Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
... new assay failed to detect acanthamoebae in 5 clini- cal specimens deemed positive by the Riviere assay, and because 3 of these organisms could not be identi- fied to the species level, we are unable to ... See full document
8
A TaqMan Probe Based Real Time PCR Assay for the Rapid Identification of the Emerging Multidrug Resistant Pathogen Candida auris on the BD Max System
... Recently, PCR-based methods for identification of C. auris infection have started to ...a PCR assay based on melt curve analysis for the detection of ...C. auris (14). Most ... See full document
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Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan Based PCR Assay
... a rapid and reproducible method for de- termination of the ...blood samples by using the TaqMan technology combined with the GeneAmp 5700 real-time sequence detection system (Applied ... See full document
6
Design and Validation of an H5 TaqMan Real Time One Step Reverse Transcription PCR and Confirmatory Assays for Diagnosis and Verification of Influenza A Virus H5 Infections in Humans
... returning from these ...by rapid nucleotide sequencing, is performed after a positive ...the development and validation of a real-time, one-step reverse transcription-PCR ... See full document
9
Rapid and Direct Real Time Detection of bla KPC and bla NDM from Surveillance Samples
... laboratory-developed PCR-based nucleic acid amplifi- cation tests (NAATs) have been described for the detection of carbapenemases in surveillance swabs and stool (8, ...(NASBA)-based assay, ... See full document
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Validation of a pan orthopox real time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood
... plaque assay method(s) and polymerase chain reaction (PCR) analysis by agarose gel electrophoresis have given way to sensitive and rapid PCR methods using minor groove binding (MGB) probe ... See full document
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Rapid Preclinical Detection of Sheeppox Virus by a Real Time PCR Assay
... A rapid diagnostic assay for CaPV would be useful for disease surveillance as well as for detection of CaPV in clinical samples and for outbreak ...(TaqMan) PCR assay ... See full document
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Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... TaqMan real-time RT-PCR assay developed in the present study offers a useful option for laborator- ies requiring a high-throughput testing ...simultaneous detection of H5 HA and N6 NA ... See full document
7
Real Time PCR Assay for Detection of Quinolone Resistant Neisseria gonorrhoeae in Urine Samples
... the development of applicable surveillance tools to detect fluoroquinolone-resistant Neisseria gonorrhoeae (QRNG) in urine ...a real-time PCR assay for detecting mutations in the ... See full document
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Diagnosis of Invasive Aspergillosis by a Commercial Real Time PCR Assay for Aspergillus DNA in Bronchoalveolar Lavage Fluid Samples from High Risk Patients Compared to a Galactomannan Enzyme Immunoassay
... Aspergillus real-time PCR assay with that of the Platelia GM assay (cutoff used, ⱖ ...specimens from lung transplant recipients, including 16 proven or probable cases of IA and ... See full document
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Diagnostic Accuracy of PCR Alone Compared to Galactomannan in Bronchoalveolar Lavage Fluid for Diagnosis of Invasive Pulmonary Aspergillosis: a Systematic Review
... separated from patients at risk (13, 34), studies with PCR sampling of pretested positive samples (35), and studies with an invalid reference standard for which we were not able to apply consensus ... See full document
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Characterization and Comparison of Galactomannan Enzyme Immunoassay and Quantitative Real Time PCR Assay for Detection of Aspergillus fumigatus in Bronchoalveolar Lavage Fluid from Experimental Invasive Pulmonary Aspergillosis
... EIA assay and qPCR assay were determined by ROC ...fluid samples from animals that received antifungal therapy (all treatment group data ...Data from continuous variables are presented ... See full document
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Bronchoalveolar Lavage Galactomannan in Diagnosis of Invasive Pulmonary Aspergillosis among Solid Organ Transplant Recipients
... Based on our data, we cannot conclusively define interpre- tive criteria for BAL GM testing. In part, this is due to the relatively small sample size and the low number of IPA cases in our study. In addition, the ... See full document
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Comparison of Real Time PCR, Conventional PCR, and Galactomannan Antigen Detection by Enzyme Linked Immunosorbent Assay Using Bronchoalveolar Lavage Fluid Samples from Hematology Patients for Diagnosis of Invasive Pulmonary Aspergillosis
... Aspergillus PCR assay. The iCycler iQ real- time detection system (Bio-Rad) was used to develop a quan- titative PCR assay for Aspergillus ...DNA from the ... See full document
5
Molecular Diagnostic Testing for Aspergillus
... serial surveillance with blood collected at weekly intervals during defined periods of ...was from a randomized study conducted in 409 HSCT recipients that compared an Aspergillus PCR-based ... See full document
6
Comparison of Methods for Detection of Vaccinia Virus in Patient Specimens
... Our real-time PCR detected vaccinia virus for a longer du- ration after vaccination of eight patients than did shell vial ...our real-time PCR using fluorescence resonance energy ... See full document
5
Development of a Rapid PCR Assay Specific forStaphylococcus saprophyticus and Application to Direct Detection from Urine Samples
... DNA from gram-negative bacilli including ...developed PCR assays for the spe- cific detection of other staphylococcal species as well as asso- ciated antibiotic resistance genes ...saprophyticus ... See full document
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