[PDF] Top 20 Direct and Quantitative Single-Cell Analysis of Human Immunodeficiency Virus Type 1 Reactivation from Latency
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Direct and Quantitative Single-Cell Analysis of Human Immunodeficiency Virus Type 1 Reactivation from Latency
... of human immunodeficiency virus type 1 (HIV-1) to establish latent infections in cells has received renewed attention owing to the failure of highly active antiretroviral therapy ... See full document
11
Single-Molecule Analysis of Human Immunodeficiency Virus Type 1 gp120-Receptor Interactions in Living Cells
... A quantitative description of the binding interactions between human immunodeficiency virus (HIV) type 1 envelope glycoproteins and their host cell surface receptors ... See full document
8
Quantitative Analysis of Human Immunodeficiency Virus Type 1-Infected CD4+ Cell Proteome: Dysregulated Cell Cycle Progression and Nuclear Transport Coincide with Robust Virus Production
... robust virus produc- tion we observed, we may point to the fact that integrase is an inherently unstable protein subject to degradation via the N- end rule (55), and that other HIV viral proteins, such as Vpr and ... See full document
13
Evidence that the Herpes Simplex Virus Type 1 ICP0 Protein Does Not Initiate Reactivation from Latency In Vivo
... derived from the isolate of strain 17syn ⫹ described ...FIG. 1. Schematic of HSV reactivation from the latent state following ...induce virus reactivation from the latent ... See full document
12
Rapid induction of apoptosis by cell-to-cell transmission of human immunodeficiency virus type 1.
... blot analysis of unintegrated DNA revealed that HIV reverse transcription was completed by 4 to 7 h following cocultivation; DNA frag- ments corresponding to one- and two-long terminal repeat circular forms of HIV ... See full document
9
Molecular Characterization of Preintegration Latency in Human Immunodeficiency Virus Type 1 Infection
... parallel analysis of linear HIV-1 DNA detected in transformed T-cell lines infected with ...which virus can be rescued from these nucle- oside-treated, HIV-1-infected, resting T ... See full document
14
Target Cell Cyclophilin A Modulates Human Immunodeficiency Virus Type 1 Infectivity
... target cell CypA interaction with HIV-1 CA is important for viral ...simultaneous analysis of CypA function with the large number of experimental tools that have accumulated over the years, including ... See full document
9
Functional Analysis of the Core Human Immunodeficiency Virus Type 1 Packaging Signal in a Permissive Cell Line
... nonpermissive cell line (11). We con- clude that the removal of any single G-rich purine motif in this region has little effect on encapsidation or ...leukemia virus and HIV-2 ap- pears to be ... See full document
11
Fluorescence Based Quantitative Methods for Detecting Human Immunodeficiency Virus Type 1 Induced Syncytia
... activated cell sorter [FACS]) to quantify HIV-induced syncytia has previously been described; however, published methods did not measure syncytia populations directly, but estimated fusion indirectly by measuring ... See full document
6
A Sensitive, Quantitative Assay for Human Immunodeficiency Virus Type 1 Integration
... prepared from graded doses of persistently infected cell lines such as 8E5 (34), U1 (10), ACH-2 (10, 29), or mixtures of such lines (37) as their stan- ...these cell lines is that they are clonal and ... See full document
9
Determinants of the Establishment of Human Immunodeficiency Virus Type 1 Latency
... latent virus in actively expressed host ...either latency establishment or ...clonal cell lines generated from our experimental system would be found inte- grated into actively transcribed ... See full document
16
Estimating Initial Viral Levels during Simian Immunodeficiency Virus/Human Immunodeficiency Virus Reactivation from Latency
... HIV reactivation after treatment inter- ruption. Analysis of HIV and SIV sequence diversity during viral rebound following interruption of combination antiretroviral therapy (cART) suggests that individual ... See full document
10
Latency and reactivation of JC virus in peripheral blood of human immunodeficiency virus type 1 infected patients
... JCV DNA was frequently detected in the leukocyte com- partment, but its amplification from plasma was possible in a few instances (12 patients), suggesting the recent production and release of viral particles. On ... See full document
5
Gamma Interferon Blocks Gammaherpesvirus Reactivation from Latency in a Cell Type-Specific Manner
... of cell type-specific effects of IFN- ␥ on ␥ HV68 ...the cell type-specific effects of IFN- ␥ lies at the level of cell type-specific responses to IFN- ␥ ...via cell ... See full document
7
Latency in Human Immunodeficiency Virus Type 1 Infection: No Easy Answers
... years. Direct examination of the viruses present in the plasma of patients with ⬍50 copies of HIV RNA/ml reveals that they are archival in nature, similar to viruses found in the reservoir, and remain sensitive to ... See full document
7
Gamma Interferon Can Prevent Herpes Simplex Virus Type 1 Reactivation from Latency in Sensory Neurons
... HSV-1 reactivation from la- tency preceded peak levels of IFN-␥ in most cultures ...HSV-1 reactivation if present shortly after ACV ...HSV-1 reactivation if HSV-1 ... See full document
7
Mutational Analysis of the Human Immunodeficiency Virus Type 1 Vif Protein
... of cell death imparted by electroporation, it proved to be infeasible to per- form fractionation studies using cells transiently cotransfected with pIIIB/Dvif- and pgVif-derived vectors (data not ...high-titer ... See full document
7
In Vivo Knockdown of the Herpes Simplex Virus 1 Latency-Associated Transcript Reduces Reactivation from Latency
... Cloning synthetic ribozymes into AAV plasmids. Selected synthetic hammerhead ribozymes targeting the LAT were cloned into scAAV2-GFP plasmids for in vitro testing and subsequent vector production. In this vector, ... See full document
10
Utility of Human Immunodeficiency Virus Type 1 Envelope as a T-Cell Immunogen
... FIG. 1. The identification of an Env-specific T-cell epitope using ...to single 15-mer peptides, of which peptides 150 and 151 both had positive responses in this ... See full document
10
The RRE of human immunodeficiency virus type 1 contributes to cell-type-specific viral tropism.
... H Replication in A3.01 cells of mutants which displayed moderately impaired replication kinetics and significant reductions in peak reverse transcriptase levels when replicated in SupTl [r] ... See full document
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