[PDF] Top 20 Dissecting the Interferon-Induced Inhibition of Hepatitis C Virus Replication by Using a Novel Host Cell Line

... HCV replication and the metabolism of the host cell), none of these mutations was found to significantly enhance HCV replication ...HCV replication. Thus, these mutations seem not to ... See full document

... by using a high-throughput siRNA trans- fection protocol as previously described ...by using a Z-score transformation so the siRNAs with the most significant effects (activation or inhibition of ... See full document

... epatitis C virus (HCV) infects over 170 million people world- wide and frequently leads to persistent infection, which in turn can lead to chronic hepatitis, cirrhosis, and hepatocellular carcinoma ... See full document

... are induced or repressed by both IFN-␥ and IFN- ␣/␤ and that are temporally linked to the disappearance of HBV ...HBV replication by IFN-␥- and IFN-␣/ ␤-dependent mechanisms, ...HBV replication is ... See full document

... After cloning this gene, we then showed that the gene product, IL-28A, has similar biological properties as other type I interferons. IL-28A resembles type I IFNs in its abil- ity to induce anti-HCV activity through ... See full document

... A novel GFP-based flow cytometry assay for HCV replica- ...HCV replication complex (18). In this replicon cell line (I/5A-GFP), the NS5A-GFP fusion protein exhibits properties of both the ... See full document

... these cell lines expressed higher levels of the liver-specific genes than the HEK293 cells used as negative controls ...liver-specific host factors in cancer cell ...AFP-expressing cell lines, ... See full document

... that hepatitis C virus, a member of the Flaviviridae family, induces the upregulation of PP2Ac in vivo (liver biopsy studies of HCV transgenic mice) and in vitro (tetracycline-regulated HCV protein ... See full document

... in cell culture differ from those in the peripheral ...IFN-␣ inhibition of mutation accumulation on HCV ge- nomes in two cultures, despite a clear effect on negative-strand HCV RNA production, was ...viral ... See full document

... chronic hepatitis C, NS5A induction of IL-8 may ultimately be involved in HCV persistence and ...holic hepatitis (44), it is tempting to speculate that NS5A induction of IL-8 may exacerbate the ... See full document

... the host, development of drug resistant virus mutants, and the lack of reproducible infectious culture systems and small animal models for HCV replication and patho- ...Although interferon-α ... See full document

... RNA replication independently from IRES-dependent translation and RNA ...the inhibition of HCV replication upon DDX60L expression sug- gested that DDX60L acts as a direct effector protein inhibiting ... See full document

... IFN-induced inhibition of HBV replication, we hypothesized that the proteasome might play a role in me- diating the antiviral ...HBV-Met cell line utilizing cell-permeable pro- ... See full document

... protein induced a marked reduction in the synthesis of envelope and capsid proteins and of HBV DNA replicative ...HBV replication at a posttranscriptional ...Indeed, using the well-characterized PRE ... See full document

... hepatoblastoma cell line HepG2 produces and secretes hepatitis B virus (HBV) after transfec- tion of cloned HBV ...HepG2 cell through binding sites in the pre-S1 ...the cell ... See full document

... with hepatitis C, which suggests that the mechanism by which IFN-alpha treatment causes viral clearance might be independent of hepatic activation of type I IFN regu- lated genes ...IFN- induced ... See full document

... In contrast, the identities of the host cell proteins which cooperate with the viral nonstructural proteins in virus replication e.g., ribosomeassociated P36, host factor, 3CD-stimulatin[r] ... See full document

... Hence, we set up an assay to crosslink NS3 to interacting proteins and to isolate these complexes (Fig.3). Replicon containing cells with (+) and without (–) a His6- tag at NS3 were incubated with increasing ... See full document

... HCV replication different from each ...maximum inhibition up to 90% at a 20 μM concentration. No significant inhibition was detected in cells transfected with the negative control siRNA ... See full document

... peg interferon, peg interferon loaded iron oxide nanoparticles and iron oxide nanoparticles, MTT assay was ...Peg Interferon and peg interferon loaded iron oxide nanoparticles were added to ... See full document