[PDF] Top 20 DNA Microarray Based PCR Ribotyping of Clostridium difficile
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DNA Microarray Based PCR Ribotyping of Clostridium difficile
... PCR ribotyping detects the lengths of ISR sequences, which in turn are determined by ISR module types and their arrangements ...the microarray operates by recognizing ISR sequence module variants and ... See full document
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Comparison of PCR based approaches to molecular epidemiologic analysis of Clostridium difficile
... by PCR methods, computer-generated images of gels were obtained by using the Den- dron software package (Solltech ...and PCR ribotyping varied considerably among isolates, patterns were compared ... See full document
5
Development and Validation of an Internationally-Standardized, High-Resolution Capillary Gel-Based Electrophoresis PCR-Ribotyping Protocol for Clostridium difficile
... Reduced PCR cycling and electrophoresis times combined with the ability to increase batch sizes (using multiple fluorescent detection dyes) represents a rela- tively rapid, high-throughput and low cost means of ... See full document
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Characterization of Toxin A Negative, Toxin B Positive Clostridium difficile Isolates from Outbreaks in Different Countries by Amplified Fragment Length Polymorphism and PCR Ribotyping
... observation. Based on the original observation, isolate 98-15323 belonged to serogroup H and had an insertion of 200 bp in the fragment amplified with primers NK11 and NK9, whereas isolate 98-15845 was found to ... See full document
7
Molecular Evaluation of the Enterotoxigenicity of Clostridium difficile and Clostridium perfringens Swine Isolates by PCR Assays
... polymorphic DNA (RAPD) typing, PCR ribotyping, and toxinotyping ...C. difficile as an epidemiology tool to identify clusters or strains that are associated with dis- ease ...of ... See full document
6
International Typing Study of Toxin A Negative, Toxin B Positive Clostridium difficile Variants
... C. difficile strain VPI 10463 and the nontoxigenic REA type M3 (isolate 1413) were chosen as the positive and negative control, ...designed based on the published sequence of the VPI 10463 pathogenicity ... See full document
5
Utilizing Rapid Multiple Locus Variable Number Tandem Repeat Analysis Typing To Aid Control of Hospital Acquired Clostridium difficile Infection: a Multicenter Study
... C. difficile selective agar plate (Oxoid, United Kingdom) and incubated anaerobically at 37°C for 48 ...C. difficile was picked for DNA extraction using the Chelex method and subcultured to blood ... See full document
5
Rapid Detection of Clostridium difficile in Feces by Real Time PCR
... Clostridium difficile is the major causative agent of nosocomial antibiotic-associated diarrhea, colitis, and pseudomembranous ...C. difficile is closely related to the production of toxins A and ... See full document
5
Correlation of Disease Severity with Fecal Toxin Levels in Patients with Clostridium difficile Associated Diarrhea and Distribution of PCR Ribotypes and Toxin Yields In Vitro of Corresponding Isolates
... PCR ribotyping. Cell pellets from overnight cultures of C. difficile were boiled in 10% Chelex, and PCR amplification of DNA interspaced between the genes encoding 16S and 23S rRNA was ... See full document
6
Evaluation of Portability and Cost of a Fluorescent PCR Ribotyping Protocol for Clostridium difficile Epidemiology
... PCR ribotyping. A list of the recommended equipment for successful PCR ribotyping is provided in Table S2 in the supplemental ...Each PCR was carried out in 25- l total volumes, and a ... See full document
6
Clostridium difficile PCR Ribotype 018, a Successful Epidemic Genotype
... Clostridium difficile infection (CDI) became a public health problem for the global spreading of the so-called hypervirulent PCR ribotypes (RTs) 027 and 078, associated with increases in the ... See full document
6
Clostridium difficile PCR Cycle Threshold Predicts Free Toxin
... tcdB PCR cycle threshold in PCR-positive toxin-positive and toxin-negative stool samples using various toxin reference ...derived based on equal weight assigned to sensitivity and specificity—are ... See full document
10
Risk factors for Clostridium difficile infection in surgical patients hospitalized in a tertiary hospital in Belgrade, Serbia: a case–control study
... analyzed PCR ribotype distri- bution of 249 C. difficile isolates received for typing from six hospital settings from South Europe (the MMA was not included) in time period from 2008 to 2015 and showed that ... See full document
6
Pathogenicity Locus, Core Genome, and Accessory Gene Contributions to Clostridium difficile Virulence
... tree based on these variants ...leaves based on acute disease ...clustered based on the clinical isolate’s clade and, to a lesser degree, MLST ...strain based on its acute virulence rank also ... See full document
15
Diagnosis of Clostridium difficile infection by toxigenic culture and PCR assay
... C. difficile strains, 15 ...C. difficile isolated from the patients with diarrhea in Babol was low ...C. difficile was reported low ...C. difficile in diarrheal patients was re- ported at ... See full document
7
Molecular and Susceptibility Analysis of Toxigenic Clostridium difficile Obtained from Adult Patients Suspected of CDI in Trinidad
... Clostridium difficile is a Gram positive rod-shaped bacterium that produces two major toxins, A and ...all Clostridium difficile positive ...for Clostridium difficile ... See full document
15
High Throughput Analysis of Gene Essentiality and Sporulation in Clostridium difficile
... C. difficile from one host to the next ...C. difficile, including the mechanism of cascade activation via Spo0A (10) and the order and timing of sigma factor activation (11, ...C. difficile, but ... See full document
13
Comparison of DNA Fingerprinting Methods for Use in Investigation of Type E Botulism Outbreaks in the Canadian Arctic
... to DNA degradation, despite the use of formaldehyde prior to DNA ...of DNA degradation, with all 21 type E isolates that were previously untypeable now producing high-quality ...interpretable ... See full document
10
Clostridium difficile infection health disparities by race among hospitalized adults in the United States, 2001 to 2010
... Keywords: Race, Disparity, Clostridium difficile Abbreviations: AHRQ, Agency for healthcare research and quality; aOR, Adjusted odds ratio; CDI, Clostridium difficile infection; CI, Conf[r] ... See full document
7
Cost of hospital management of Clostridium difficile infection in United States—a meta analysis and modelling study
... We carried out meta-analysis for cost studies following a Monte Carlo simulation approach, as reported by Jha et al [23] and Zimlichman et al [17], bearing in mind the het- erogeneity of the included studies. For each ... See full document
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