[PDF] Top 20 Duck hepatitis B virus infection of hepatocytes is not dependent on low pH.
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Duck hepatitis B virus infection of hepatocytes is not dependent on low pH.
... 4, we also investigated the effects of NH4Cl on infection by DHBV in experiments in which i hepatocytes were washed with pH 2.2 buffer at the end of inoculation to prevent further intern[r] ... See full document
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Time- and Temperature-Dependent Activation of Hepatitis C Virus for Low-pH-Triggered Entry
... ingly, in contrast to the data presented here, Hsu et al. re- ported that HCVpp (genotype 1a, strain H77) were moderately susceptible to inactivation by low pH (17). The basis for this difference is unknown ... See full document
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Glucagon Treatment Interferes with an Early Step of Duck Hepatitis B Virus Infection
... hepadnavirus infection was studied in vitro with the duck hepatitis B virus (DHBV) ...throughout infection or starting up to 8 h after virus uptake resulted in a ... See full document
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Initiation of Duck Hepatitis B Virus Infection Requires Cleavage by a Furin-Like Protease
... DHBV infection of primary duck ...the duck liver, suggesting the possible involvement of the endosome in switching DHBV binding from DCPD to DGD followed by membrane ...susceptible duck liver ... See full document
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Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent
... human hepatocytes (PHH). (A and B) HCV-positive serum S297 or S298 was preincubated for 30 min at 4°C with 10, 20, or 40 g/ml green monkey (G), mouse (M), or human (H) CD81-LEL fusion proteins and, 3 days ... See full document
6
Uptake of duck hepatitis B virus into hepatocytes occurs by endocytosis but does not require passage of the virus through an acidic intracellular compartment.
... A pH-independent membrane fusion which requires endo- cytosis is rather uncommon for enveloped ...is pH independent and takes place at the outer cell membrane ...Sendai virus) or is pH de- ... See full document
5
A Metastable Form of the Large Envelope Protein of Duck Hepatitis B Virus: Low-pH Release Results in a Transition to a Hydrophobic, Potentially Fusogenic Conformation
... under low-pH conditions which rapidly trigger the hydrophobic conformation resulted in membrane binding ...similar low-pH-dependent membrane binding occurred with cell monolayers, ... See full document
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Entry of Hepatitis B Virus into Immortalized Human Primary Hepatocytes by Clathrin-Dependent Endocytosis
... human hepatocytes provides a clue to the cause of the disordered metabolism of iron ...primary hepatocytes could be performed to analyze differentially expressed genes and proteins responsible for ... See full document
11
Duck hepatitis B virus infection of Muscovy duck hepatocytes and nature of virus resistance in vivo.
... For staining of DHBV envelope proteins, cells were fixed in methanol-acetone 1:1 and stained with a mixture of two mouse monoclonal antibodies, 1H.1 and 7C.12, that are specific for the [r] ... See full document
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Viral DNA-Dependent Induction of Innate Immune Response to Hepatitis B Virus in Immortalized Mouse Hepatocytes
... epatitis B virus (HBV) has infected approximately 2 billion people worldwide, with 350 million of them becoming chronically infected ...chronic hepatitis, cirrhosis, and hepatocellular carcinoma ... See full document
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Scavenger Receptor Class B Type I and Hepatitis C Virus Infection of Primary Tupaia Hepatocytes
... natural infection of hepatocytes by native ...primary hepatocytes with native virions are needed to verify findings obtained with HCVpp and other surrogate systems mimicking the early steps of viral ... See full document
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Effect of Antiviral Treatment with Entecavir on Age- and Dose-Related Outcomes of Duck Hepatitis B Virus Infection
... of hepatocytes that stained positive for DHBsAg were counted in liver biopsy tissue with the aid of an eyepiece graticule in 200 250- by 250- m grid fields (containing ⬃100,000 hepatocytes) and were ... See full document
14
Genomic responses to hepatitis B virus (HBV) infection in primary human hepatocytes
... Raw methylation data was imported and processed using R/Bioconductor packages [37, 38]. Data quality was inspected using boxplots for the distribution of methylated and unmethylated signals, and inter-sample relationship ... See full document
15
Efficient Infection of Primary Tupaia Hepatocytes with Purified Human and Woolly Monkey Hepatitis B Virus
... Nycodenz gradient purification of HBV particles. In all experiments described here, the iodinated bencoic acid derivate Nycodenz (batch 10032780; Nycomed Pharma AS, Oslo, Norway) was dissolved in phosphate-buffered ... See full document
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Clonal Expansion of Normal-Appearing Human Hepatocytes during Chronic Hepatitis B Virus Infection
... infected hepatocytes by antiviral cytotoxic T lymphocytes (CTLs) ...infected hepatocytes, which should initially constitute the entire hepa- tocyte ...altered hepatocytes that had lost the ability to ... See full document
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Detection of Clonally Expanded Hepatocytes in Chimpanzees with Chronic Hepatitis B Virus Infection
... hepadnavirus infection, viral DNA integrates at a low rate into random sites in the host DNA, producing unique virus-cell junctions detectable by inverse nested PCR ...individual hepatocytes, ... See full document
13
Proteomic analysis of primary duck hepatocytes infected with duck hepatitis B virus
... DHBV infection in PDHs was deter- mined by indirect immunofluorescence and Southern blot ...DHBV infection was observed by the confocal laser scanning microscope ... See full document
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n-Butyrate, a cell cycle blocker, inhibits early amplification of duck hepatitis B virus covalently closed circular DNA after in vitro infection of duck hepatocytes.
... nucleic acid binding capacity (12) and lead to destabilization of the nucleocapsid, allowing release of the genome into the nucleus and cccDNA formation. Third, butyrate may exert a more direct role in cccDNA ... See full document
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Recombinant Duck Interferon Gamma Inhibits Duck Hepatitis B Virus Replication in Primary Hepatocytes
... human hepatitis B virus (HBV), the woodchuck hepatitis virus (WHV), and the duck hepatitis B virus ...during infection with other viruses, modulate ... See full document
7
Hepatocytes Traffic and Export Hepatitis B Virus Basolaterally by Polarity-Dependent Mechanisms
... hepadnaviruses hepatitis B virus (HBV) and duck hepatitis B virus (DHBV) in polarized hepatocyte-derived cell lines and primary duck ...was dependent on the ... See full document
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