[PDF] Top 20 Efficient and Heritable Gene Targeting in Tilapia by CRISPR/Cas9
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Efficient and Heritable Gene Targeting in Tilapia by CRISPR/Cas9
... that Cas9-mediated cleavage can be abol- ished by single mismatch at the gRNA–target site interface, particularly in the last 10–12 nucleotides located in the 39 end of the 20-nt gRNA targeting ...the ... See full document
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A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Hordeum vulgare L.)
... the Cas9 gene for expression in barely cells The sequence of the Cas9 gene from Streptococcus pyo- genes (Gene Bank accession AMH03982) was optimized for expression in monocot plants ... See full document
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Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas
... the CRISPR/Cas system was made by the adaption of addi- tional Cas9 nucleases to genome ...used Cas9 nuclease originates from Streptococ- cus ...that Cas9 orthologues from other species are ... See full document
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Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system
... the CRISPR/Cas9 system in two plasmids To establish a genome editing system in ...on CRISPR/Cas9, we designed and constructed a two-plasmid system that separated Cas9 and the sgRNA into ... See full document
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Tracking and transforming neocortical progenitors by CRISPR/Cas9 gene targeting and piggyBac transposase lineage labeling
... an efficient method to deliver multiple transgenes into rodent neural progenitors in the developing ...the CRISPR/Cas9 system with IUE and piggyBac transposase lineage labeling to induce somatic ... See full document
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In Vitro CRISPR/Cas9 System for Efficient Targeted DNA Editing
... The CRISPR/Cas9 system facilitates site-specific cleavage of the chromosome from various ...the CRISPR/Cas9 system has been developed as a universal genome-editing tool and has been widely ... See full document
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A Multiplex CRISPR/Cas9 System for Use as an Anti-BmNPV Therapeutic
... the CRISPR/Cas9 system is multiple genome ...single Cas9 enzyme targeting two or more sgRNA at different genomic sites for simultaneous production of multiple DNA ...multiplex ... See full document
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Efficient Editing of Malaria Parasite Genome Using the CRISPR/Cas9 System
... the CRISPR/sgRNA-transfected parasites (see ...Py03652 gene with the gfp gene in Plasmodium ...the Cas9 and sgRNA expression cassettes and donor template for HR repair after double-strand ... See full document
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Efficient genome engineering of Toxoplasma gondii using CRISPR/Cas9
... with CRISPR/Cas9 plasmids or with control plasmids lacking a targeting protospacer in the presence or absence of the Cas9 ...a targeting protospacer, and further improved in the absence ... See full document
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CRISPR–Cas9 a boon or bane: the bumpy road ahead to cancer therapeutics
... understanding gene function. CRISPR/Cas9 is a targeted genome-editing platform derived from bacterial adaptive immune system and has been repurposed into a genome-editing ...endonuclease, Cas9 ... See full document
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Highly efficient heritable targeted deletions of gene clusters and non coding regulatory regions in Arabidopsis using CRISPR/Cas9
... of CRISPR/Cas9 in generating genomic deletions, Arabidopsis plants were transformed with the pUbiCAS9-Red vector containing sgRNA pairs targeting twelve distinct genomic ...the targeting ... See full document
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Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9
... using CRISPR/CAS9. Previous studies have shown that combin- ing CRISPR/CAS9 with sgRNAs can be used to generate site- specific double-strand breaks in the target DNA that are repaired by NHEJ, ... See full document
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CRISPR/Cas9 TECHNOLOGY A NEW BOON IN GENOME EDITING
... the CRISPR/Cas9 system for genome editing in mammalian cell lines and zebrafish embryos showed that the technology was at least as efficient as ZFNs and TALENs targeting the same sites and in ... See full document
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Evolution of Resistance Against CRISPR/Cas9 Gene Drive
... It is clear that there is a need for more detailed modeling of CGD on several fronts. For example, we assumed that re- sistance is a binary trait, yet resistance levels could depend on number, type, and location of ... See full document
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A CRISPR Interference System for Efficient and Rapid Gene Knockdown in Caulobacter crescentus
... optimized CRISPR interference (CRISPRi) for use in ...pasteurianus CRISPR systems, we show that the coexpression of a catalytically dead form of Cas9 (dCas9) with a single guide RNA (sgRNA) ... See full document
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HIV-1: tackling the obstacles that limit the effectiveness of CRISPR-Cas9 gene editing of the T cell co-receptor CCR5 Tatiana Hillman 1https://orcid.org/0000-0002-7539-0512
... PREVENTING CRISPR CAS9-SGRNA OFFTARGET MODIFICATIONS Hu et ...engineered CRISPR-Cas9 to delete sites in the HIV-1 LTR U3 region, ceasing gene expression and replication within HIV-1 ... See full document
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Efficient gene deletion and replacement in Aspergillus niger by modified in vivo CRISPR/Cas9 systems
... nuclease Cas9 can cause a double-strand break (DSB) in the target deoxyribonucleic acid (DNA) which is formed by a 20-bp DNA sequence of target gene with a 5 ′ -NGG nucleotide sequence in the subsequent ... See full document
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Precise and Heritable Genome Editing in Evolutionarily Diverse Nematodes Using TALENs and CRISPR/Cas9 to Engineer Insertions and Deletions
... pyogenes cas9 gene, which is codon op- timized for eukaryotic expression and carries a C-terminal HA-2xNLS-mCherry-2xNLS fusion tag, was constructed as ...the cas9 fusion gene from pDD921 ... See full document
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Crispr/Cas9 System - A Revolution in Gene Editing
... All things we had to harvest from the organs of animals before that. During their evolution bacteria have developed a set of barriers to protect themselves against invaders such as phage and plasmid nucleic acids. ... See full document
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Specifically blocking the fatty acid synthesis to inhibit the malignant phenotype of bladder cancer
... The CRISPR-related (CAS) system is a special system that exists in certain bacteria to remove foreign genetic ...II CRISPR system, nuclease Cas9 can be widely used in mammalian cells for ... See full document
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