[PDF] Top 20 Epstein-Barr virus (EBV)-negative B-lymphoma cell lines for clonal isolation and replication of EBV recombinants.

... The response to induction of BL30, BL41, and Loukes clones infected with T1E2-HYG P3HR1 recombinants, assessed by expression of gp350/220, indicated Table 2 that they were all more permi[r] ... See full document

... and replication assays in C666-1 ...42 cell doublings (6 to 84 days), and processed as described in the legend for ...the EBV genomes in C666-1 ...3 cell doublings (6 days) before (Input) and ... See full document

... panel B, the Western blots were probed with human β-Actin and lamin A/C-specific ...Burkitt lymphoma; LCL: lymphoblastoid cell line; NPC: nasopharyngeal carcinoma cell ...of cell ... See full document

... large B-cell lymphoma (ALK-LBCL), and ...before EBV testing by EBERs in situ ...exclusively EBV- negative and does not exhibit clonal rearrangement of the immunoglobulin ... See full document

... Moreover, the EBV-infected Blymphoma cells are often partially permissive for early replicative cycle gene expression and virus replication can be induced, in contrast to previously repo[r] ... See full document

... all EBV-associated malignancies have a predominantly latent pattern of viral gene expression led to the assumption that only the latent phase of viral gene expression is important during the development of ... See full document

... Human B-cells can routinely be transformed in cell culture by infection with ...human B-cells with equivalent numbers of infectious units of BPLF1- knockout virus resulted in decreased ... See full document

... The cell pellet was resuspended by sonification in solubilization buffer ...mono- clonal antibodies. Figure 4 shows Western blot analysis of lymphoma-derived cell line P3HR1/16 and ... See full document

... of EBV-positive BL, EBV infec- tion is also considered as an additional pathogenetic ...isolated EBV-positive and EBV-neg- ative clones in vitro from a t(8;14)(q24;q32)-carrying BL cell ... See full document

... lytic replication of EBV in B cells have been obtained predominantly after reactivation with chemical treat- ment or by superinfection of the cells ...the EBV genome via concatemeric ...of ... See full document

... all virus isolates from any of the human populations stud- ied were either uniformly type 1 at all four polymorphic loci (EBNA2, -3A, -3B, and -3C) or uniformly type 2 (16, 18, 21, ...occurring recombinants ... See full document

... of Epstein-Barr virus (EBV) strains into types 1 and 2 is based on linked polymorphisms in the EBV nuclear antigen 2 (EBNA2) gene, situated in the BamHI Y region of the genome, and in ... See full document

... Epstein-Barr virus (EBV) is a ubiquitous human gammaher- pesvirus that establishes life-long latent infections in B lym- phocytes following the primary infection (16, ...23). EBV ... See full document

... were separated by discontinuous gel electrophoresis and blot- ted on Protran filters (Schleicher and Schuell) (35). EBNA-1 was detected with a polyclonal human serum (diluted 1:5 in milk) by incubating the filters with ... See full document

... of EBV DNA containing the entire BHRF1 gene including 5 ⬘ and 3 ⬘ untranslated regions (accession number NC_007605; nucleotides [nt] 41492 to 43130) was amplified by high-fidelity ...of EBV ... See full document

... a B cell lymphoblastoid cell line (LCL) named HKLY-28 was established from NPC tissue from a Hong Kong Chinese patient ...(5). Virus from this line was passaged to B cells from the ... See full document

... 328 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The random association of Epstein-Barr virus DNA with host cell metaphase chromosomes of all sizes in Burkitt's lymp[r] ... See full document

... of EBV replication in Akata cells resulted in these arrays detecting most LTIII ...in EBV- transformed lymphoblastoid ...on EBV DNA ... See full document

... of B lymphocytes but not for infection of epithelial cells ...niche, EBV binding to the cell surface utilizes a specific interaction be- tween the type 2 complement receptor CR2 (CD21) and gp350/220, ... See full document

... 6069-6073 0022-538X/94/$04.00+0 Copyright © 1994, American Society for Microbiology Isolation of Epstein-Barr Virus EBV-Negative Cell Clones from the EBV-Positive Burkitt's Lymphoma BL L[r] ... See full document