[PDF] Top 20 Establishment of reverse genetics system for PPR virus to develop recombinant vaccines
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Establishment of reverse genetics system for PPR virus to develop recombinant vaccines
... Using reverse genetics techniques RNA virus genomes can be modified through cDNA copies of their ...chimeric vaccines elicited protective immune responses in goats challenged with virulent ... See full document
237
Live attenuated virus vaccines for respiratory syncytial and parainfluenza viruses: applications of reverse genetics
... Reverse genetics has also been used to determine the genetic basis of attenuation of a Jennerian PIV3 vaccine candidate, that is, a BPIV3 wild-type virus that is anti- genically related to human PIV3 ... See full document
8
Development of a reverse genetics system for respiratory syncytial virus long strain and an immunogenicity study of the recombinant virus
... RSV vaccines are ...for reverse genetics manipulation in discovering “ideal” attenuated RSV candidates and also allows examining the contribution of each viral compo- nent to vaccine-enhanced ... See full document
16
All-in-One Bacmids: an Efficient Reverse Genetics Strategy for Influenza A Virus Vaccines
... LAIV vaccines are trivalent or quadrivalent products aimed at providing protection against H1 and H3 influenza A virus sub- types and influenza type B ...Influenza virus reverse ... See full document
13
Attenuation of the virulence of a recombinant influenza virus expressing the naturally truncated NS gene from an H3N8 equine influenza virus in mice
... influenza virus (EIV) causes a highly contagious disease in horses and other ...a reverse genetics system was applied to generate various NS gene recombinant viruses using the backbone ... See full document
9
Establishment of Canine RNA Polymerase I-Driven Reverse Genetics for Influenza A Virus: Its Application for H5N1 Vaccine Production
... pandemic, vaccines whose antigenicities match those of circulating strains must be rapidly ...alternative reverse genetics system for influenza virus using the canine polymerase I ... See full document
5
IBV : potential as a vaccine vector and identification of a novel subgenomic mRNA
... the virus genome and thus requires the development of a reverse genetics system for the ...coronavirus reverse genetics systems were hampered; partly due to the large size of ... See full document
247
Research on basis of reverse genetics system of a Sindbis like virus XJ 160
... vector system of XJ- 160 virus, recombinant vector together with its helper plasmid pBR-H were derived from XJ-160 viral infec- tious clone pBR-XJ160 by overlap-PCR ...vector system is capable ... See full document
5
Reverse Genetics System for Severe Fever with Thrombocytopenia Syndrome Virus
... syndrome virus (SFTSV) is an emerging tick-borne pathogen that was first reported in China in ...SFTS virus represents a new lineage within the Phlebovirus genus, distinct from the existing sandfly fever ... See full document
12
Development and Application of a Reverse Genetics System for Japanese Encephalitis Virus
... Construction of full-length infectious cDNAs for JEV. Recombinant DNA techniques were used according to standard procedures (31). Three overlapping cDNA amplicons (JVF, JVM, and JVR) originally used for complete ... See full document
16
A plasmid-based reverse genetics system for influenza A virus.
... A reverse genetics system for negative-strand RNA viruses was first successfully developed for influenza ...influenza virus-infected ...plasmid-driven system results in the efficient ... See full document
5
Establishment of a reverse genetics system for Schmallenberg virus, a newly emerged orthobunyavirus in Europe
... a recombinant virus was created lacking NSs ...(containing recombinant virus, which was named rSBVdelNSs) was harvested 5 days after transfection for use in a plaque assay in BHK-21 ... See full document
9
Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells
... to develop an efficient influenza plasmid- based rescue system in MDCK ...plasmid system based on a T7 RNA polymerase vector has been reported, this system has not been documented to work for ... See full document
12
Rift Valley Fever Virus Lacking the NSs and NSm Genes Is Highly Attenuated, Confers Protective Immunity from Virulent Virus Challenge, and Allows for Differential Identification of Infected and Vaccinated Animals
... (RVF) virus is a mosquito-borne human and veterinary pathogen associated with large outbreaks of severe disease throughout Africa and more recently the Arabian ...The virus S segment- encoded NSs and the M ... See full document
11
Engineering Recombinant Orsay Virus Directly in the Metazoan Host Caenorhabditis elegans
... elegans-based reverse genetics system to generate recombinant mutants of Orsay ...Orsay virus, for example, by fusion of green fluorescent protein (GFP) or intro- duction of a ... See full document
8
Characterization of the Polyproline Region of the Hepatitis E Virus in Immunocompromised Patients
... proteasome system to ensure the efficient re- cruitment of virus protein to the viroplasm ...the virus life cycle ...of PPR ubiquitination on the regulation of virus replication must ... See full document
9
Generation of Recombinant Rotaviruses Expressing Fluorescent Proteins by Using an Optimized Reverse Genetics System
... Generation of recombinant RVAs from only 11 RVA rescue T7 plasmids. The standard protocol was performed basically as described by Komoto et al. (10). The major modifications were (i) that newly constructed 10 T7 ... See full document
15
Importance of Neuraminidase Active-Site Residues to the Neuraminidase Inhibitor Resistance of Influenza Viruses
... (H3N2) recombinant virus background, we characterized seven charged, conserved NA residues (R118, R371, E227, R152, R224, E276, and D151) that directly interact with the NAIs but have not been reported to ... See full document
9
Requirements and comparative analysis of reverse genetics for bluetongue virus (BTV) and African horse sickness virus (AHSV)
... RG method for AHSV was slightly modified by lowering the amount of plasmids from 600 to 300 ng, and by using optimized AHSV-ORFs in expression plasmids. Many more virus variants were rescued with modified RG ... See full document
9
Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus
... of recombinant virus (1). The second system involved the in vitro assembly of the TGEV full-length cDNA by using a series of contiguous cDNAs with engineered unique restriction ...infectious ... See full document
11
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