[PDF] Top 20 Evaluation of Antigen-Specific T-Cell Responses with a Miniaturized and Automated Method
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Evaluation of Antigen-Specific T-Cell Responses with a Miniaturized and Automated Method
... Reagents. Phosphate-buffered saline (PBS) and RPMI 1640 medium were purchased from BioWhittaker, Verviers, Belgium. RPMI 1640 medium was en- riched with 10 mM L -glutamine and with 5% fetal calf serum selected for low ... See full document
8
Myocardial infarction triggers cardioprotective antigen specific T helper cell responses
... cardiac T cell infiltration showed substantial similari- ties across species, and the numbers of infiltrating cardiac T cells peaked at the healing ...different cell types demands careful ... See full document
16
Antigen specific Tregs control T cell responses against a limited repertoire of tumor antigens in patients with colorectal carcinoma
... antitumor T cell responses in cancer patients are strongly controlled by Tregs, and increased numbers of tumor-infiltrating Tregs correlate with reduced ...tumor-specific T cell ... See full document
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Oral Vaccination with Attenuated Salmonella enterica Strains Encoding T-Cell Epitopes from Tumor Antigen NY-ESO-1 Induces Specific Cytotoxic T-Lymphocyte Responses
... the cell surface. A highly efficient gene replacement method was used to generate peptide vaccines based on Salmonella enterica serovar Typhimurium ...The T-cell epitopes (NY-ESO-1 p157-165 ... See full document
6
An inducible transgenic mouse breast cancer model for the analysis of tumor antigen specific CD8 T-cell responses
... of T-Ag NP positive cells in involuted ...as T-Ag NP expressing cells are scarce ...in T-Ag NP positive cells after immune- suppressive treatment would be much easier to detect than in NP8 mice in ... See full document
17
Efficient Generation of Mucosal and Systemic Antigen-Specific CD8+ T-Cell Responses following Pulmonary DNA Immunization
... ⫹ T cells in the lungs and MLN of mice immunized by the pulmonary route 12 weeks following homologous boost, and these mice were protected against a lethal intranasal vaccinia virus ...⫹ T cells confer ... See full document
11
Oral administration of an immunodominant T cell epitope downregulates Th1/Th2 cytokines and prevents experimental myasthenia gravis
... 431A automated peptide synthesizer (Applied Biosystems Perkin-Elmer Europe ...primary antigen-specific responses from lymph node T cells (T-cell tolerance) and (b) the ... See full document
10
An oral recombinant Salmonella enterica serovar Typhimurium mutant elicits systemic antigen-specific CD8+ T cell cytokine responses in mice
... The polymerase chain reaction for amplification of gfp was conducted in a 50 μl volume with 4.5 units AmpliTaq Gold™ DNA polymerase (Applied Biosystems), 1× PCR buffer, 1.5 μM of each primers (GFP2 and GR), 0.2 mM ... See full document
10
A role of cellular glutathione in the differential effects of iron oxide nanoparticles on antigen-specific T cell cytokine expression
... immune responses, and induce oxidative stress in ...attenuated antigen-specific immunity in vivo, though the underlying mechanism remains ...on antigen-specific cytokine expression by ... See full document
8
Antigen-Specific T-Cell Responses of Leprosy Patients
... immune responses of leprosy patients ...further evaluation, DNAs encoding selected ...rate specific restriction enzyme sites 5 ⬘ and 3 ⬘ of the gene of interest and excluded in the target gene for ... See full document
7
Co-administration with DNA encoding papillomavirus capsid proteins enhances the antitumor effects generated by therapeutic HPV DNA vaccination
... enhanced antigen-specific CD8 + in both CRT/E7 and ovalbumin (OVA) antigenic ...+ T cell immune ...+ T cell immune responses by L1 DNA also confers improved therapeutic ... See full document
10
Effect of anti-IgE therapy on food allergen specific T cell responses in eosinophil associated gastrointestinal disorders
... Allergen specific T cell responses were measured using a polychromatic adaptation of published flow cytometry methods utilizing carboxyfluorescein succini- midyl ester (CFSE) dye dilution ... See full document
8
Comparison of Immunologic Assays for Detecting Immune Responses in HIV Immunotherapeutic Studies: AIDS Clinical Trials Group Trial A5181
... 12 responses from these assays were used to analyze differences using fresh and cryopreserved ...B-lymphoblastoid cell lines (B-LCLs) as antigen-presenting cells (APCs) (ELISPOT ... See full document
8
Polyethylene glycol-coated graphene oxide attenuates antigen-specific IgE production and enhanced antigen-induced T-cell reactivity in ovalbumin-sensitized BALB/c mice
... on antigen-specific immunity, antibody production and T-cell reactivity in OVA-sensitized mice were ...OVA- specific IgM, IgG 1 , and IgG 2a were not altered (Table ...Th ... See full document
10
Intravenous administration of the selective toll-like receptor 7 agonist DSR-29133 leads to anti-tumor efficacy in murine solid tumor models which can be potentiated by combination with fractionated radiotherapy
... This is the first paper describing the novel small molecule TLR7 agonist DSR-29133 (structure defined in Figure 1A). Using NF-kB/SEAP/293 cells stably transfected with either human TLR7 or TLR8 (or mouse TLR7, ... See full document
12
Enhanced Induction of Intestinal Cellular Immunity by Oral Priming with Enteric Adenovirus 41 Vectors
... immune responses than the negative control group in both compartments ...tetramer responses than single rAd5 vaccine- immunized mice in the spleen ...these responses more effectively ...immune ... See full document
9
Chimeric murine polyomavirus virus-like particles induce Plasmodium antigen-specific CD8+ T cell and antibody responses
... FIGURE 2 | Structural analysis of in vitro assembled chimeric murine polyomavirus virus-like particles. Wild and chimeric MuPyVP1-G4S proteins were expressed in E. coli and purified by liquid chromatography. Proteins ... See full document
12
In vivo microRNA-155 expression influences antigen-specific T cell-mediated immune responses generated by DNA vaccination
... Total RNA was purified from 1 × 10 7 BMDCs by acid- phenol:chloroform extraction using the mir Vana miRNA Isolation Kit (Ambion, Austin, TX). The resultant sam- ple was enriched for small RNA species by ethanol pre- ... See full document
11
The Early Kinetics of Cytomegalovirus-Specific CD8+ T-Cell Responses Are Not Affected by Antigen Load or the Absence of Perforin or Gamma Interferon
... immune responses participate in the control of murine cytomegalovirus (mCMV) ...ⴙ T cells. mCMV-specific CD8 ⴙ T-cell responses are unusual in that, even after mCMV has been ... See full document
7
Type I Interferon Signaling Prevents Hepatitis B Virus-Specific T Cell Responses by Reducing Antigen Expression
... ⫹ T cell responses are required for the clearance of ...mune responses to the induction of HBV-specific CD8 ⫹ T cell responses are not fully un- ...⫹ T cell ... See full document
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