[PDF] Top 20 Evaluation of the Xpert MTB/RIF Ultra Assay for Direct Detection of Mycobacterium tuberculosis Complex in Smear Negative Extrapulmonary Samples

... 168 smear-negative extrapulmonary samples (Table 1), obtained from 148 adult patients and collected between May 1999 and May 2017, were analyzed in the Department of Microbiology at the ... See full document

... RT MTB in combination with the add-on resistance assay, RT MTB INH/RIF, is a novel fully automated real-time PCR system for high-throughput diagnostics of TB, including resis- tance markers ... See full document

... was smear negative and culture negative but positive by both the IS6110 LDT and Xpert MTB/RIF ...nary tuberculosis 6 months prior to presenting with seizures and an ... See full document

... the direct head-to-head comparison of Xpert MTB/RIF with two widely used commercial NAATs, ...CTM- MTB uses real-time PCR technology like Xpert MTB/ RIF but is ... See full document

... of Xpert MTB/RIF assay for direct detection of Mycobacterium tuberculosis (Mtb) and rifampin resistance in pulmonary and extrapulmonary ... See full document

... of tuberculosis (TB) cases in India are estimated to have extrapulmonary disease, and due to a lack of diagnostic means, they often remain ...early detection of Mycobacterium ... See full document

... CSF samples had M. tuberculosis LODs similar to those of saline solution in the GeneXpert ...CSF samples (median, 25 CFU/ ml) was lower than that reported for spiked sputum samples (131 ... See full document

... The Xpert MTB/RIF (Xpert) assay permits rapid near-patient detection of Mycobacterium tuberculosis in sputum; however, the test sensitivity remains suboptimal in ... See full document

... The Xpert MTB/RIF (Xpert) assay (Cepheid ...molecular assay, which permits rapid TB diagnosis through detection of the DNA of Mycobacterium tubercu- losis and ... See full document

... either Xpert® MTB/RIF cartridges or the next-generation Xpert MTB/RIF Ultra ...The Ultra cartridge showed significantly better performance (increased sensitivity) ... See full document

... INNO-LiPA RIF TB (Innogenetics, Ghent, Belgium) and the Genotype MTBDRplus (Hain Life-Sci- ence, Nehren, Germany) (here referred as to ...and Xpert MTB/RIF assays have shown good performance ... See full document

... of smear-positive sputum samples, Xpert is somewhat less sensitive when testing smear-negative ...when Xpert was performed follow- ing negative smear microscopy ... See full document

... M. tuberculosis clumping from the MGIT-grown cultures being better trapped by the Xpert MTB/RIF filter membrane, whereas an ...M. tuberculosis single cell ( ⬃ ... See full document

... Pulmonary samples (sputum, bronchoalveolar lavage, gastric lavage) and extrapulmonary samples (pus, aspirates, body fluids, lymph node [LN] tissue) were collected in special, plain, universal 30ml ... See full document

... also smear neg- ative, but its ADA value was high and its tuberculin test result was ...were smear positive and the patients who provided the specimens were clinically diagnosed with active TB, the ... See full document

... For the per sample analyses, confidence intervals for accuracy measures and comparisons of sensitivities of different tests are based on marginal logistic regression with an identity link function. Of note, using ... See full document

... of smear microscopy include its simplic- ity, low cost, speed, and high specificity in high tuberculosis bur- den ...addition, smear microscopy identifies the most in- fectious people with ... See full document

... of samples (n=2741) were obtained and processed under standardised and highly monitored conditions, as shown by the robust and high-quality data generated at both study sites for the Xpert ... See full document

... Both direct and concentrated smears were prepared, fixed, and stained by Ziehl Neelsen Staining before examining under oil immersion lens for the presence or absence of the Acid Fast Bacilli ...concentrated ... See full document

... (except Mycobacterium marinum, Mycobac- terium kansasii, Mycobacterium szulgai, and Mycobacterium flave- ...and negative controls; if either control fails, the result is deemed indeterminate ... See full document