[PDF] Top 20 Evaluation of efficacy of Dried Blood Spots (DBS) as compared to plasma samples for the detection of HIV-1 drug resistance mutations
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Evaluation of efficacy of Dried Blood Spots (DBS) as compared to plasma samples for the detection of HIV-1 drug resistance mutations
... for HIV resistance, genotypic assays are the gold standard and plasma is the most appropriate clinical sample (12), since it is known to have HIV-1 RNA at higher and more stable levels ... See full document
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Development and Evaluation of an Affordable Real Time Qualitative Assay for Determining HIV 1 Virological Failure in Plasma and Dried Blood Spots
... of HIV-1 antiretroviral treatment (ART) ...and evaluation of a low-cost, dried blood spot (DBS)-compatible qualitative assay to determine VF, in accordance with current WHO guideline ... See full document
7
Evaluation of the Aptima HIV 1 Quant Dx Assay Using Plasma and Dried Blood Spots
... an evaluation of Aptima for the semiquantitative detection of HIV-1 RNA in paired DBS and EDTA plasma samples from patients on ...Aptima compared to RT-PCR methods for DBS ... See full document
5
Feasibility of Detecting Human Immunodeficiency Virus Type 1 Drug Resistance in DNA Extracted from Whole Blood or Dried Blood Spots
... type 1 (HIV-1) drug resistance testing in resource-poor settings is still ...whole blood or dried blood spots (DBS). Samples were collected from 50 ... See full document
10
Field Evaluation of Dried Blood Spots for Routine HIV 1 Viral Load and Drug Resistance Monitoring in Patients Receiving Antiretroviral Therapy in Africa and Asia
... a plasma VL of ⬍3,000 copies/ml, likely due to proviral DNA and intracellular RNA ...a plasma VL of ⬍ 3,000 copies/ml (18, ...on HIV Quantification is used in many laboratories in developing ... See full document
9
Evaluation of dried blood spots as a feasible alternative to plasma for detection, quantification and genotyping of hepatitis C virus: A pilot study
... 98 plasma and DBS pairs were compared and HIV-1 RNA was detected in 100% of DBS samples when plasma viral loads were >3000 copies/ml ...serological detection of ... See full document
153
Evaluation of Different RNA Extraction Methods and Storage Conditions of Dried Plasma or Blood Spots for Human Immunodeficiency Virus Type 1 RNA Quantification and PCR Amplification for Drug Resistance Testing
... between plasma and DPS or ...Amplicor HIV-1 Monitor ...observed compared to the results for ...genotypic drug resistance testing (12, 19), but never for viral load measurements, ... See full document
12
Correlation between Human Immunodeficiency Virus Type 1 (HIV 1) RNA Measurements Obtained with Dried Blood Spots and Those Obtained with Plasma by Use of Nuclisens EasyQ HIV 1 and Abbott RealTime HIV Load Tests
... of detection for HIV RNA in DBS by either of the two commercial assays was greater than ...log HIV RNA ...of plasma viremia of greater than 3 log units while a patient is receiving antiretro- ... See full document
6
Use of Dried Blood Spot Samples and In House Assays To Identify Antiretroviral Drug Resistance in HIV Infected Children in Resource Constrained Settings
... of HIV genotyping of DBS samples obtained from HIV-infected children enrolled in clinical trials in sub-Saharan Africa (1, ...11). HIV genotypes for reverse transcriptase were ... See full document
6
Evaluation of Dried Blood Spots for Human Immunodeficiency Virus Type 1 Drug Resistance Testing
... Dried blood spots (DBS) are simpler to prepare, store, and transport than plasma or serum and may represent a good alternative for drug resistance genotyping, particularly in ... See full document
5
Both Human Immunodeficiency Virus Cellular DNA Sequencing and Plasma RNA Sequencing Are Useful for Detection of Drug Resistance Mutations in Blood Samples from Antiretroviral Drug Naive Patients
... infected drug-naive subjects, and the material of choice is plasma ...Since drug resistance mutations (DRMs) may persist longer in cellular DNA than in plasma RNA, we ... See full document
7
HIV 1 drug resistance and drug dependence
... the drug- dependence phenomenon can also be used in the preclin- ical testing of improved entry inhibitors, which should preferentially not stimulate the T20-dependent HIV-1 var- ... See full document
7
A hepatitis C avidity test for determining recent and past infections in both plasma and dried blood spots
... DBS sent to the WoSSVC for anti-HCV and HCV PCR test- ing and subsequently stored at 4 ◦ C were used in this study. The samples came from clinics run by community addition ser- vices. Group 4 consisted of 65 DBS ... See full document
7
Quantifying the fitness cost of HIV-1 drug resistance mutations through phylodynamics.
... among HIV-infected people, and improved their quality of ...include drug resistance, drug toxicity, or poor treatment ...transmitted drug resistance by analysing the viral ... See full document
16
Pooled PCR testing of dried blood spots for infant HIV diagnosis is cost efficient and accurate
... approach compared to individual DBS ...period 1 January 2009 to 31 July 2015 were used for the simulation (Add- itional file ...10 samples for individual testing and 20 samples when pooling ... See full document
6
Rapid Detection of Common HIV 1 Drug Resistance Mutations by Use of High Resolution Melting Analysis and Unlabeled Probes
... successful drug resistance surveillance and testing in resource-limited settings are cost, access to equipment, and ...key HIV-1 drug resistance codons in samples ... See full document
12
Transmitted drug resistance, selection of resistance mutations and moderate antiretroviral efficacy in HIV 2: Analysis of the HIV 2 Belgium and Luxembourg database
... of HIV-2 infected persons live in West Afri- can countries [13], where HAART is not yet available or has only been implemented ...for plasma viral load quantification and no commercial assay is ...first ... See full document
11
Detection of Human Immunodeficiency Virus Type 1 DNA in Dried Blood Spots by a Duplex Real Time PCR Assay
... The HIV sequence that we targeted for real-time detection is in the well-conserved portion of the ...standard HIV-1 subtype panels (consisting of eight HIV-1 sub- types, A ... See full document
7
Set up of Genotyping Test for Detection of HIV-1 Drug Resistance
... resistant HIV-1 isolates and is one of the earliest applications of gene sequencing for clinical purposes ...of HIV-infected patients under ...sequence HIV-RNA from plasma ... See full document
5
Evaluation of the Aptima HCV Quant Dx Assay Using Serum and Dried Blood Spots
... and Drug Adminis- tration (FDA) approved for the confirmation of active HCV infection and for monitoring HCV viral load using serum and ...amplicon detection using fluorescently labeled ... See full document
7
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