[PDF] Top 20 Expression of active human immunodeficiency virus type 1 protease by noninfectious chimeric virus particles.
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Expression of active human immunodeficiency virus type 1 protease by noninfectious chimeric virus particles.
... Attempts to detect RT in MoHIV-I and MoHIV-III cell lysates and virion particles by immunoblot analysis were consistently negative under conditions in which the protein was readily detec[r] ... See full document
8
Inhibitors of human immunodeficiency virus type 1 zinc fingers prevent normal processing of gag precursors and result in the release of noninfectious virus particles.
... (5a). Virus pellets were isolated by centrifugation of supernatants for 1 h at 18,000 3 g and 4 8 C after clarification of supernatants for 10 min at 250 3 g and 4 8 ...the virus pellet was lysed by ... See full document
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Conditional human immunodeficiency virus type 1 protease mutants show no role for the viral protease early in virus replication.
... producing virus particles at different temperatures and then quantitating the amount of virus produced at each tempera- ...infectious virus particles produced from cells transfected at ... See full document
5
Nef association with human immunodeficiency virus type 1 virions and cleavage by the viral protease.
... viral particles produced by the Gag polyprotein of MLV, which does not encode a Nef-like ...protein. Particles produced by the MLV Gag polyprotein expression construct lacked other virion components, ... See full document
6
Chimeric Human Immunodeficiency Virus Type 1 Containing Murine Leukemia Virus Matrix Assembles in Murine Cells
... efficient virus replication. Although entry, in- tegration, and expression of virus proteins occur efficiently, virion assembly and Gag polyprotein processing are severely impaired, resulting in ... See full document
8
Autoprocessing of human immunodeficiency virus type 1 protease miniprecursor fusions in mammalian cells
... in virus-like particles (VLPs) released into the cul- ture medium were examined as an indirect measure- ment of protease activity and/or autoprocessing ...HIV protease construct (Figure 2B ... See full document
10
Lack of integrase can markedly affect human immunodeficiency virus type 1 particle production in the presence of an active viral protease.
... of human immunodeficiency virus type 1 is not required for efficient viral particle assembly or ...formed particles with normal ...viral protease (PR) gene which prevented ... See full document
6
Incorporation of High Levels of Chimeric Human Immunodeficiency Virus Envelope Glycoproteins into Virus-Like Particles
... of chimeric Env ...encoding chimeric Env were ini- tially constructed for comparison ...gp120 expression and secretion in an insect cell system ...the chimeric constructs H-CT MMTV and M-CT ... See full document
10
Contribution of Virus-Like Particles to the Immunogenicity of Human Immunodeficiency Virus Type 1 Gag-Derived Vaccines in Mice
... MMLV–HIV-1 chimeric Gag that releases signif- icant amounts of VLPs when expressed in murine cells (13, ...VLP-producing expression vectors (MHGag) to prime antigen-specific responses compared with ... See full document
12
Trans-packaging of human immunodeficiency virus type 1 genome into Gag virus-like particles in Saccharomyces cerevisiae
... that noninfectious VLPs encap- sidating gene of interest can be produced in a yeast cell system and may provide clues to the development of yeast VLP vaccines that confer ensured safety and enhanced im- ...tested ... See full document
13
Monitoring processed, mature Human Immunodeficiency Virus type 1 particles immediately following treatment with a protease inhibitor-containing treatment regimen
... both noninfectious, immature virus particles and virions found in ...a protease inhibitor using a simple ...of protease inhibitors in vitro, and may be useful in evaluating new drugs ... See full document
8
Generation of Infectious Feline Immunodeficiency Virus (FIV) Encoding FIV/Human Immunodeficiency Virus Chimeric Protease
... Retroviral protease (PR) is responsible for the temporal processing of viral Gag and the Gag-Pol polyprotein into struc- tural and enzymatic proteins during viral maturation (2, ...with human ... See full document
11
A p6Pol-protease fusion protein is present in mature particles of human immunodeficiency virus type 1.
... are active in their precursor form or how their activation is regu- ...leukemia virus NC-PR fusion protein, which lacks the cleavage site between the adjacent proteins, is inactive in ver- tebrate cells ... See full document
5
Chimeric Plant Virus Particles as Immunogens for Inducing Murine and Human Immune Responses against Human Immunodeficiency Virus Type 1
... with virus carrying plasmid pPVX-2F5E or wild-type (WT) PVX by abrading the surfaces of two leaves per plant with carborundum and inoculating each leaf with 20 g of plasmid ...correct expression of ... See full document
6
Partial inhibition of the human immunodeficiency virus type 1 protease results in aberrant virus assembly and the formation of noninfectious particles.
... By 10 h after infection, the cells infected with the untreated virus contained approximately 5,000 copies of viral DNA lane 5, whereas the cells newly infected with virus produced in the[r] ... See full document
6
Use of a Novel Chimeric Mouse Model with a Functionally Active Human Immune System To Study Human Immunodeficiency Virus Type 1 Infection
... for human immunodefi- ciency virus type 1 (HIV-1) infection are arguably the nonhu- man primate models for simian immunodeficiency virus and chimeric ... See full document
6
Human immunodeficiency virus type 1 Vpr: oligomerization is an essential feature for its incorporation into virus particles
... The oligomerization feature of Vpr was evaluated by using a complementation system based on Venus protein as a reporter. A strategy involving the generation of chi- meric Venus-Vpr protein has allowed us to monitor oli- ... See full document
11
Replicative Fitness of Protease Inhibitor-Resistant Mutants of Human Immunodeficiency Virus Type 1
... mutant virus documented by the experimental data in the present study agrees with a prior study of the protease enzyme function of this mutant ...mutant virus in the ab- sence of the drug will likely ... See full document
9
Second locus involved in human immunodeficiency virus type 1 resistance to protease inhibitors.
... HIV-1 protease inhib- itor-resistant ...in protease-mutated viruses and pro- vide a mechanism by which HIV-1 could compensate for im- paired protease ...of protease activity ... See full document
8
Altered Substrate Specificity of Drug-Resistant Human Immunodeficiency Virus Type 1 Protease
... I84V substitution, and HIV PRs 945 and 1049 each had a V82A substitution. Although residues 82 and 84 form part of the S1 pocket and not the S2 pocket, a reductive mutation at either position could cause a shift in bound ... See full document
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