[PDF] Top 20 Gene Expression Pattern of Human Chorion Derived Mesenchymal Stem Cells during Adipogenic Differentiation

... acid binding protein 4 (FABP4) and Glycerol-3-phosphate dehydrogenase (GPD2) gene were purchased from Applied Biosystems (Foster City, CA, USA). RT-PCR was moni- tored using the 7900 HT Fast Real-Time PCR System ... See full document

... 5 cells from the monolayer was cultured for 3 weeks in adipogenic medium consisting of low-glucose Dulbecco’s modified Eagle’s medium (DM EM; Sigma) with 1-mM dexamethasone (Sigma), ...weeks, ... See full document

... that cells from middle-aged donors could ...the adipogenic-differentiation potential of OASCs was ...staining-positive cells but also the mRNA expression of PPAR γ , a key transcription ... See full document

... The human cysteine rich protein 61 (CYR61, CCN1) as well as the other members of the CCN family of genes play important roles in cellular processes such as proliferation, adhesion, migration and ...in human ... See full document

... with human umbilical cord mesenchymal stem cells (hUCMSCs) after transfected with recombinant human insulin gene ...insulin gene transfection on the adipogenesis of ... See full document

... of cells and new bone matrix, as indicated by increased deposition of osteopontin, osteocalcin, and bone ...the differentiation of hESC-derived MPs in osteogenic ...increased gene ... See full document

... shown human ADMSCs can differentiate into cartilage cells provided that they are cultured on a three-dimensional matrix and in a medium containing special regulator materials (dexamethasone, TGF-β) capable ... See full document

... Tbx20 expression on osteo- genesis, Ad-MSCs were transduced with LV-Tbx20, which expresses both Tbx20 and EGFP, or with LV-Ctrl, which only codes for ...transduced cells were subse- quently cultured in ... See full document

... Improved sequencing platforms and approaches have recently led researchers to understand that tRNA loci can also give rise to sncRNA molecules, with these tRNA frag- ments (tRFs), which are also known as ... See full document

... the adipogenic and osteogenic lineage commit- ment clearly involve distinct master transcriptional regu- lators and downstream genetic cascades, in many cases, they are also regulated by the same signaling ... See full document

... the expression levels of miR- 138 and mRNAs, U6 small nuclear RNA (U6, for miRNA) and glyceraldehyde-phosphate dehydrogenase (GAPDH, for mRNAs) were used as endogenous controls, according to the compara- tive ... See full document

... adipocyte differentiation. To determine the role of adipogenic miRNAs in the adipocyte differentiation process, we used microarray technology to monitor miRNA levels in human ... See full document

... Autoantibody in the circulation is mainly immunoglobulin G (IgG), which is responsible for the formation of immune complex. The IgG immune complex may elicit tissue destruction by two mechanisms, either through the ... See full document

... hMuStem cells. Interestingly, passage of hMuStem cells hPL in HS-supplemented medium resulted in a rate of differentiation comparable to that observed in hMuS- tem cells HS , indicating that ... See full document

... biological function of MSCs derived from human chori- onic villus, human bone marrow, and human adipose. Although MSCs from different source share similar anti- gens, their repair potential ... See full document

... the expression levels of cell proliferation- related genes during in vitro MSC culture are important for the capability of these cells to further proliferate and differentiate [78, 81, ...therefore, ... See full document

... HEK293 cells were transfected with constructed reporter vectors simultaneously with plasmid vector (Promega, Madison, WI, USA) containing cDNA coding renilla luciferase, which is driven by CMV ...transfection, ... See full document

... Abstract: Objective: In the current study, a hypoxia and/or serum-free model was constructed in vitro to identify the optimal protocol for hUC-MSCs expansion, while still maintaining growth capacities. Methods: Parallel ... See full document

... differentiated cells mimicking the formation of embryonic bodies observed with ESCs or ...a differentiation media supplemented with Activin and WNT3a activators, followed by 3-day incubation in a media ... See full document

... and differentiation towards neural and skeletal lineages) for these stem cells, many of these studies included the use of hMSCs [25, 46, 47], unrestricted somatic stem cells [48] and ... See full document