[PDF] Top 20 Expression profiling with RNA from formalin-fixed, paraffin-embedded material
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Expression profiling with RNA from formalin-fixed, paraffin-embedded material
... selected from the literature [14] and from microarray results ...[15]. Expression was measured from intact and FFPE-derived RNA and raw Cts from all the 14 tumors are plotted for ... See full document
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Original Article MicroRNA Expression Profiling Outperforms mRNA Expression Profiling in Formalin-fixed Paraffin-embedded Tissues
... modifications RNA undergoes as a result of formalin fixation show that the principle change includes the addition of a hydroxymethyl group (termed “methylol”) to the nucleic acid ...derived from ... See full document
9
Microarray analysis of RNA extracted from formalin-fixed, paraffin-embedded and matched fresh-frozen ovarian adenocarcinomas
... gene expression profiling of FFPE samples on microarrays has some ...differential expression was typically higher in FFPE than in FF samples, suggesting higher level of noise in the FFPE ...gene ... See full document
11
Expression profiling of formalin-fixed paraffin-embedded primary breast tumors using cancer-specific and whole genome gene panels on the DASL®platform
... Reference RNA (UHRR) samples (Agilent Technologies, Santa Clara, CA) comprised from 10 human cell lines were used as control RNA samples to assess the quality of RNA labeling and hybridization ... See full document
16
miRNA expression profiling in formalin-fixed paraffin-embedded endometriosis and ovarian cancer samples
... of RNA transcripts ~19–22 nucleotides in length, the altered miRNA pattern being connected to pathological ...assessed from formalin-fixed paraffin-embedded (FFPE) samples ... See full document
14
Detection of Survivin Gene Expression in Formalin-Fixed Paraffin-Embedded Tissues of Human Osteosarcoma: Its Potential Usefulness in Diagnosis and Prognosis of Bone Tumors
... Hemi nested RT-PCR reaction. The same amount of the extracted RNA (24 l) from each sample was used for cDNA synthesis. Reverse transcription was performed by reverse transcriptase enzyme (Fermentase Co., ... See full document
7
Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
... gene expression profiling of FTCs with metastases or local recur- ...signal from a small amount of neoplastic tissue might be dominated by the surrounding normal thyroid ... See full document
10
Analytical validation of a prognostic prostate cancer gene expression assay using formalin fixed paraffin embedded tissue
... included. RNA was isolated from 55 prostate tumour FFPE CNB (n = 15) and RP (n = 40) samples followed by microarray genomic profiling on both ...an RNA sequencing platform (Roche NimbleGen ... See full document
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Impact of the G84E variant on HOXB13 gene and protein expression in formalin-fixed, paraffin-embedded prostate tumours
... tumours from both PcTas12–3 and PcTas72–6 were well- to moderately-differentiated with GS < 6, whereas where there was no evidence for variant allele transcription, the tumours were predominantly poorly ... See full document
9
Gene-expression analysis of a colorectal cancer-specific discriminatory transcript set on formalin-fixed, paraffin-embedded (FFPE) tissue samples
... protein expression level changes between normal and CRC samples ...in RNA Tm’s of 87 °C, 84 °C, 85 °C, 86 °C, ...(21’mer, RNA Tm 84 °C), see Jørgensen and collaborators, 2011 ...obtained from ... See full document
12
Analysis of microRNA from archived formalin-fixed paraffin-embedded specimens of amyotrophic lateral sclerosis
... miRNAs from cerebrospinal fluid (CSF) [6,34,37], peripheral blood [33,34,37-39] and skeletal muscle ...of RNA can be reduced through cross-linking with proteins, enzyme degradation as well as chemical ... See full document
11
Validation of DNA methylation profiling in formalin-fixed paraffin-embedded colon and breast tissue using the Infinium HumanMethylation450 Microarray
... between formalin and DNA emphasizes the necessity for careful evaluation of methylation microarrays before they can be confidently used on formalin-fixed sample ...sample material in ... See full document
12
Validation of the MethylationEPIC BeadChip for fresh-frozen and formalin-fixed paraffin-embedded tumours
... effects from storage, other studies also report no significant correlation between age of FFPE samples and the performance of methylation arrays [9, 11, ... See full document
6
Indoleamine 2,3-dioxygenase expression and overall survival in patients diagnosed with breast cancer in Pakistan
... IDO expression was higher in the TNBC patients compared to that in the non-TNBC ...IDO expression is considerably linked with overall decreased patient survival and poor prognosis in patients diagnosed ... See full document
7
Journal of Applied Pharmaceutical Science
... biomarkers could serve as intermediate markers of disease in clinical trials, and also be possible drug targets. Identification of clinically significant protein biomarkers of phenotype and biological function is an ... See full document
10
Evaluation of formalin-fixed paraffin-embedded tissues in the proteomic analysis of parathyroid glands
... ranged from 8 (G-protein gamma-12 subunit and ATP synthase subunit e) to 305 kDa (thyroglobulin), while the pI ranged from ...both from the number of peptides found for each identified protein ... See full document
10
Single-day HER2neu amplification assessment using chip-based digital PCR in formalin-fixed paraffin-embedded breast carcinoma tissue
... obtained from targeted manual microdissection along with low sample input requirements is enough to offset this ...obtained from this study suggest that all IHC equivocal samples could likely be tested by ... See full document
9
Targeted next-generation sequencing of head and neck squamous cell carcinoma identifies novel genetic alterations in HPV+ and HPV- tumors
... Sequencing of HPV DNA demonstrated 100% concor- dance of HPV status. All samples were laser-capture microdissected (LCM) to separate tumor epithelial from surrounding stromal tissues, enriching tumor DNA for ... See full document
12
Whole genome sequencing of genotype VI Newcastle disease viruses from formalin fixed paraffin embedded tissues from wild pigeons reveals continuous evolution and previously unrecognized genetic diversity in the U S
... sequences from tissues collected from MT, MA, and PA that clustered with a portion of isolates collected from northeastern states, and the other group had sequences from samples collected ... See full document
11
Identification by 16S rRNA Gene Analyses of a Potential Novel Mycobacterial Species as an Etiological Agent of Canine Leproid Granuloma Syndrome
... directly from clinical specimens of canine lep- roid granuloma ...isolated from 13 lesion specimens from 12 dogs, and RCBH identified this sequence in a further 4 ...dog from which both ... See full document
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