[PDF] Top 20 Expression and self-assembly of empty virus-like particles of hepatitis E virus.

... Hepatitis E virus (HEV), a member of the family Caliciviri- dae, is one of the causative agents of viral acute hepatitis formerly known as enterically transmitted non-A, non-B hep- ...atitis. ... See full document

... proteins self- assembled into virus-like particles (VLPs) that were released into the cell supernatant and purified by CsCl gradient ...native virus capsid and reacted with pig ... See full document

... pORF2 expression in Sf9 and Tn5 cells may allow us to understand the mechanism underlying the subunit assembly and particle formation of the recombinant HEV ... See full document

... fusion expression system offers advan- tages over other fusion technologies, including improved soluble expression, proper folding, protection from degradation, and simplified purification and detection ... See full document

... on virus-like particles (VLPs) with good safety and immunogenicity is ...authentic virus capsid under the transmission electron microscopy (TEM), which is also in accordance with the dynamic ... See full document

... Norwalk virus (NV) is the prototype strain of a group of human caliciviruses responsible for epidemic outbreaks of acute ...models, expression of the capsid protein in insect cells results in the ... See full document

... were plated, allowed to adhere and incubated separately with 250 nM (saturation binding concentration based on our earlier work [1]) of various RNA-VLP complexes. At various time points, post incubation (12, 24, 36, 48, ... See full document

... released particles, but their detection required significantly increased exposure times ...and E). The levels of the RSV G and F proteins in these particles were dramatically reduced compared to the ... See full document

... of particles generated by glycoprotein expression was next determined by dynamic light scattering (Zetasizer Nano ZS; Malvern ...of virus-like structures does not require the participation of ... See full document

... C virus (HCV) is a major cause of chronic hepatitis and cirrhosis and has been shown to be a causative agent in hepatocellular ...the virus was first identified in 1989 ...(ssRNA) virus of the ... See full document

... facilitated assembly of the HCV proteins into VLPs, as determined both biophysically and ...ing expression of HCV Core, E1, and E2 in insect cells using a baculovirus vector and in cells infected with a ... See full document

... vaccinia virus infection/transfection protocol and a plasmid transfection-only protocol was not cell type specific, parallel experiments were performed with HeLa T4 ...VLP assembly and budding. VLP release ... See full document

... coronavirus E protein is likely to play an important role in virus ...the E and M proteins from either mouse hepatitis virus (MHV) or transmissible gastroenteritis virus (TGEV) ... See full document

... C-E1-E2-p7 expression cassette [BacC-p7(1b)] ...enveloped particles, detergent-free lysates prepared from transduced Huh7 cells were incubated with recombinant CD81 large extracellular loop (LEL) fused to ... See full document

... apoE expression by an apoE- specific siRNA resulted in reductions of infectious HCV in the cells and in the medium by 10- and 100-fold, respectively ...single-cycle virus growth assay which measured only ... See full document

... into particles ranging in size from 16 to 22 nm in diameter, which falls within the size range reported for infectious virions (10–22 nm in diameter) ...example, expression of the full-length BFDV CP using ... See full document

... template-driven assembly using fusion proteins ...cage assembly pathway ...of hepatitis B core assembly have used a truncated Cp incorporating the N-terminal assembly domain ...the ... See full document

... diverse virus families like Picornaviridae (poliovirus), Alphaviridae (Sindbis virus, Semliki Forest virus, and Venezuelan equine encephalitis vi- rus), Coronaviridae (human coronavirus E229), ... See full document

... SDM. Expression plasmids (p-HBs-T116N and p-HBs-G130N) based on the pCI vector (Promega, Madison, WI) encoding HBsAgS pro- teins (genotype D, serotype ayw) with T116N and G130N amino acid substitutions were ... See full document

... this virus is able to effectively evade innate and adaptive immune responses, including ...reducing expression of key transcription factors (19, ...ncreased expression of a complement reg- ulator(s) ... See full document