[PDF] Top 20 Genome sequence and description of Alistipes senegalensis sp. nov.

... Open Reading Frames (ORFs) were predicted us- ing Prodigal [21] with default parameters but the predicted ORFs were excluded if they were span- ning a sequencing GAP region. The predicted bac- terial protein sequences ... See full document

... specimen was preserved at −80 °C after collection and sent to Marseille. Strain JCE T (Table 1) was isolated in July 2013 by cultivation on blood culture bottle (Becton Dickinson, Temse, Belgique) supplemented with rumen ... See full document

... er type strains within the quences were aligned using CLUSTALW, and phylogenetic inferences obtained using the maximum- likelihood method within the MEGA software. Numbers at the nodes are bootstrap values obtained by ... See full document

... Clostridium ihumii strain AP5 T (=CSUR P198 = DSM 26098) is the type strain of C. ihumii sp. nov. This bac- terium is a Gram-positive, anaerobic rod-shaped bacteria that was isolated from the stool sample ... See full document

... P162, DSM 25372, was grown aerobically on 5% sheep blood-enriched BHI agar at 37°. Four petri dishes were spread and growth from the plates was resuspended in 3x500µl of TE buffer and stored at 80°C. Then, 500µl of this ... See full document

... rRNA sequence similarity of 94.8 % 16S rRNA sequence identity with Haemophilus parasuis, the phylogenetically closest species with a name with standing in nomenclature and a poor MALDI-TOF MS score ...a ... See full document

... dilution was performed such that 1 ng of each genome was used to prepare the paired end library. The “tag- mentation” step fragmented and tagged the DNA with a mean size of 1.5 kb. Then limited cycle PCR ... See full document

... Microbacterium laevaniformans strain OR221 (AJGR00 000000), Microbacterium luticocti strain DSM 19459 (AULS00000000), Microbacterium paraoxydans strain 77MFTsu3.2 (AQYI00000000), Microbacterium testaceum strain StLB037 ... See full document

... other type strains within the genus Anaerococcus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using CLUSTALW, and phylogenetic inferences obtained using the maximum- likelihood method ... See full document

... Description of sp. nov. (gro.sen ′ sis. L. gen. masc. n. grossensis , of gros, the French adjective for fat, as the strain was isolated from an obese patient). Colonies are 1 mm in diameter on ... See full document

... their genome sequence, MALDI-TOF spectrum and main phenotypic char- acteristics (habitat, Gram-stain reaction, cultiva- tion conditions, cell wall structure and metabolic ... See full document

... complete genome sequence and annotation. The 3,425,960 bp long genome (1 chromo- some but no plasmid) contains 3,064 protein-coding and 49 RNA ... See full document

... senegalensis and [5-11], thus suggesting that the human digestive flora is far from being fully known. The fecal specimen was preserved at -80°C after collection and sent to Marseille. Strain JC225 (Table 1) was ... See full document

... This latter value was lower than the 98.7% 16S rRNA gene sequence threshold recommended by Stackebrandt and Ebers to delineate a new species without carrying out DNA-DNA hybridization [40]. Different growth ... See full document

... Approximately 790,000 beads were loaded on 1/4 region of a GS Titanium PicoTiterPlate PTP Kit 70x75 and sequenced with the GS-FLX Titanium Sequencing Kit XLR70 (Roche). The run was per- formed overnight and then analyzed ... See full document

... The genome of C. massiliensis was compared with those of C. intestinalis, C. aerofaciens, C. stercoris, C. tanakaei, Eggerthella lenta and Coriobacterium glomerans (Table 6). The draft genome of C. ... See full document

... P147 = DSMZ 25959) is the type strain of S. anaer- obia gen. nov., sp. nov. This bacterium was isolat- ed from the feces of a healthy Senegalese patient. It is a Gram-positive, anaerobic, ... See full document

... the genera Micrococcus, Arthrobacter and Kocuria [35]. The Nesterenkonia genus includes Gram- positive, non spore-forming, aerobic, mesophilic bacteria that may be halotolerant or halophilic. Currently, the genus ... See full document

... nucleotide sequence similarity with ...gene sequence threshold recommended by Stackebrandt and Ebers to delineate a new species without carrying out DNA-DNA hybridization ...nucleotide sequence ... See full document

... type strains within the quences were aligned using CLUSTALW, and phylogenetic inferences obtained using the maximum- likelihood method within the MEGA software. Numbers at the nodes are bootstrap values obtained by ... See full document