[PDF] Top 20 Herpes Simplex Virus Type 1 Cytoplasmic Envelopment Requires Functional Vps4
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Herpes Simplex Virus Type 1 Cytoplasmic Envelopment Requires Functional Vps4
... infectious virus yields it is always important to determine whether the observed reduction is due to the failure to assemble virus particles or due to the assembly of particles that have reduced ...of ... See full document
8
Differing Roles of Inner Tegument Proteins pUL36 and pUL37 during Entry of Herpes Simplex Virus Type 1
... in virus egress has been well documented, their role in virus entry is less easy to ...the virus capsids leaving an infected nucleus can gain direct access to uninfected nuclei without needing to ... See full document
12
UL20 Protein Functions Precede and Are Required for the UL11 Functions of Herpes Simplex Virus Type 1 Cytoplasmic Virion Envelopment
... in cytoplasmic envelopment, we constructed the ⌬ UL20/UL11 double-null ...double-null virus exhibited replication characteristics very similar to those of the UL20-null ...double-null virus ... See full document
12
Herpes simplex virus type 2 infection triggers AP 1 transcription activity through TLR4 signaling in genital epithelial cells
... Functional TLR4 are located on the surface of cell mem- brane for sensing extracellular stimuli, such as bacteria LPS. We then extracted total proteins from the membrane or cytoplasm of U937 cells (human leukemic ... See full document
14
Herpes Simplex Virus Type 1 UL34 Gene Product Is Required for Viral Envelopment
... efficient envelopment at the inner nuclear membrane have been ...apparently requires prior completion of the DNA packaging process. In wild-type virus-infected cells, empty capsids are only ... See full document
13
Cytoplasmic Residues of Herpes Simplex Virus Glycoprotein gE Required for Secondary Envelopment and Binding of Tegument Proteins VP22 and UL11 to gE and gD
... Secondary envelopment of F-BAC gD ⴚ /gE CT ...final envelopment poorly and, instead, accumulate large numbers of nucleocap- sids in aggregates in the cytoplasm ...1,000 virus particles in multiple ... See full document
13
Herpes Simplex Virus Type 1 Production Requires a Functional ESCRT-III Complex but Is Independent of TSG101 and ALIX Expression
... The virus was then concentrated by centrifugation and purified on a 5 to 15% Ficoll ...of virus particles was collected by side ...of virus par- ticles as estimated by the PFU, or the same volume for ... See full document
11
Herpes Simplex Virus 1 UL37 Protein Tyrosine Residues Conserved among All Alphaherpesviruses Are Required for Interactions with Glycoprotein K, Cytoplasmic Virion Envelopment, and Infectious Virus Production
... The herpes simplex virus 1 (HSV-1) UL37 protein functions in virion envelopment at trans-Golgi membranes, as well as in retro- grade and anterograde transport of virion ... See full document
11
Herpes Simplex Virus 1 Protein UL37 Interacts with Viral Glycoprotein gK and Membrane Protein UL20 and Functions in Cytoplasmic Virion Envelopment
... for virus replication and spread on BD45 cells transformed with an HSV-1 gene fragment containing the UL37 gene ...DC480 virus was also comple- mented for virus replication and virus ... See full document
9
Functional Hierarchy of Herpes Simplex Virus 1 Viral Glycoproteins in Cytoplasmic Virion Envelopment and Egress
... Herpes simplex virus 1 (HSV-1) viral glycoproteins gD (carboxyl terminus), gE, gK, and gM, the membrane protein UL20, and membrane-associated protein UL11 play important roles in ... See full document
9
Interaction of herpes simplex virus glycoprotein gC with mammalian cell surface molecules.
... of herpes simplex virus (HSV) into mammalian cells is a multistep process beginning with an attachment step involving glycoproteins gC and ...step requires the interaction of glycoprotein gD ... See full document
13
A Pre-Immediate-Early Role for Tegument ICP0 in the Proteasome-Dependent Entry of Herpes Simplex Virus
... Reduced capsid transport to the nucleus in the absence of tegument ICP0. To begin to address the impact of virion ICP0 on HSV entry, we analyzed HSVs bearing mutations in the ICP0 gene (Table 1). Viruses with ICP0 ... See full document
9
Infection of Chinese Hamster Ovary Cells by Pseudorabies Virus
... Lack of gD-mediated interference in infection of CHO cells by PrV. Cell lines expressing gD exhibit a distinct resistance to infection by homologous and sometimes heterologous alpha- herpesviruses (2–4, 12). This ... See full document
8
A Gene Capable of Blocking Apoptosis Can Substitute for the Herpes Simplex Virus Type 1 Latency-Associated Transcript Gene and Restore Wild-Type Reactivation Levels
... ocular herpes simplex virus type 1 (HSV-1) infection, the virus travels up axons and establishes a lifelong latent infection in neurons of the trigeminal ...HSV-1 ... See full document
12
Pseudorabies Virus Glycoprotein M Inhibits Membrane Fusion
... Fig. 1A, after transfection of only the gB-expressing plasmid, fluorescent single cells and no polykaryocytes were observed, which indicates that gB alone is not sufficient to promote cell fusion. In contrast, ... See full document
9
Heptad Repeat 2 in Herpes Simplex Virus 1 gH Interacts with Heptad Repeat 1 and Is Critical for Virus Entry and Fusion
... Herpes simplex virus 1 (HSV-1) entry into cells and cell-cell fusion mediated by HSV-1 glycoproteins require four glycoproteins, gD, gB, gH, ...a functional HR-2 capable ... See full document
10
Cellular Proteasome Activity Facilitates Herpes Simplex Virus Entry at a Postpenetration Step
... relative importance of an active site depends on the substrate (37). MG132 and lactacystin target the chymotrypsin-like ac- tivity primarily but do inhibit the remaining catalytic activities to a lesser extent (38). ... See full document
10
Postentry Events Are Responsible for Restriction of Productive Varicella-Zoster Virus Infection in Chinese Hamster Ovary Cells
... One striking characteristic of VZV-infected CHO-K1 cells is the strictly nuclear localization of IE62. IE62 staining in NIH 3T3 cells also appeared to be nuclear (Fig. 1). Phosphorylation of IE62 by a viral ... See full document
10
Human astrovirus infection associated with encephalitis in an immunocompetent child: a case report
... following hours seizures reoccurred. She received leve- tiracetam intravenously up to a total dose of 50 mg/kg per day without a lasting effect. Full blood count, elec- trolytes, ammonia, blood gas analysis, inflammatory ... See full document
5
The ESCRT Related ATPase Vps4 Is Modulated by Interferon during Herpes Simplex Virus 1 Infection
... and VPS4 complexes participate ...and VPS4 results in autophagosome accumulation in neurons and other cells (21, 22, ...and VPS4 activity also limits HSV-1 replication ...and/or Vps4 ... See full document
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