[PDF] Top 20 High throughput real time PCR based genotyping without DNA purification
Has 10000 "High throughput real time PCR based genotyping without DNA purification" found on our website. Below are the top 20 most common "High throughput real time PCR based genotyping without DNA purification".
High throughput real time PCR based genotyping without DNA purification
... hours, without including the time it then takes to normalize each individual ...for genotyping without requiring DNA ...multiplex PCR experiments ... See full document
6
High Throughput Pooling and Real Time PCR Based Strategy for Malaria Detection
... for real-time PCR assays for malaria in a cohort of 182 pregnant women in ...a real-time PCR assay that detects all Plasmodium species, microscopy- positive samples were ... See full document
8
Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR
... of DNA-tag amplification in samples (background ...obtain high level of amplification in the samples containing ana- lyte comparing to the negative controls ...of PCR tubes and the pretreatment ... See full document
10
A Magnetic Bead-Based DNA Extraction Protocol Suitable for High-Throughput Genotyping in Shrimp Breeding Programs
... shrimp DNA extracted using the SDE protocol in combination with the AccuBead, Silanol or Sera-Mag bead magnetic bead types (Table 3) was genotyped using 2 MassARRAY panels (Sellars et ...the time for ... See full document
10
A Rapid and High Throughput Screening Approach for Methicillin Resistant Staphylococcus aureus Based on the Combination of Two Different Real Time PCR Assays
... SA442 PCR resulted in a threshold cycle (C T ) value of 39 or lower in combination with a positive mecA or mecC PCR (C T value, ⬍ ...SA442 PCR were interpreted as negative, since an in-house ... See full document
7
Real Time Quantitative PCR for Human Herpesvirus 6 DNA
... the real-time HHV-6 DNA detection assay described herein offers several advantages over the traditional QC-PCR method: first, the time required for labor, costs, and sample handling ... See full document
7
Modeling of 5′ Nuclease Real Time Responses for Optimization of a High Throughput Enrichment PCR Procedure for Salmonella enterica
... nuclease real-time PCR assay was studied to optimize an automated method of detection of preenriched Salmonella enterica cells in buffered peptone water ...the PCR reagents were evaluated on ... See full document
9
Detection and Genotyping of Varicella Zoster Virus by TaqMan Allelic Discrimination Real Time PCR
... TaqMan real-time PCR assay to detect and differentiate wild-type virus from Oka vaccine strains of ...VZV DNA could be genotyped and quantified within minutes of thermocycling completion due ... See full document
5
Comparison of Real Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection
... of DNA extraction methodology on the performance of HPV detection systems have been published to ...of DNA specimen tested, exam- ined the impact of alternate extraction systems (Qiagen DNA blood and ... See full document
8
Optimization of Quantitative Detection of Cytomegalovirus DNA in Plasma by Real Time PCR
... plasma-based PCR assay for CMV DNA using a high-throughput TaqMan system and the Qiagen Robot 3000 automated DNA extraction ... See full document
7
Fluorescence resonance energy transfer-based real-time polymerase chain reaction method without DNA extraction for the genotyping of F5, F2, F12, MTHFR, and HFE
... extension time depended on the size of the amplicon for each ...the PCR, we used asymmetric primer concentration, which prevented the PCR signal from reach- ing ...the PCR FRET-based ... See full document
8
Protocol: a versatile, inexpensive, high-throughput plant genomic DNA extraction method suitable for genotyping-by-sequencing
... automation without a fume hood ...solvent, high-throughput DNA extraction protocol suitable for PCR-based assays from freeze- dried plant and insect ...quality high ... See full document
10
Automated Extraction of Viral Pathogen RNA and DNA for High Throughput Quantitative Real Time PCR
... estimated time required to perform the manual and m1000 extractions is presented in Table ...setup time for the m1000 system was ...total time and 210-min de- crease of hands-on time was ... See full document
6
Buccal cells DNA extraction to obtain high quality human genomic DNA suitable for polymorphism genotyping by PCR-RFLP and Real-Time PCR
... bjective: The aim of this study was to evaluate, by PCR-RFLP and Real-time PCR, the yield and quality of genomic DNA collected from buccal cells by mouthwash after different storage time[r] ... See full document
5
Genotyping of Chlamydophila psittaci by Real Time PCR and High Resolution Melt Analysis
... The current study reports the development and implemen- tation of a novel diagnostic assay that is capable of rapidly genotyping C. psittaci. A three-marker panel (Ppac, GTpc, and GT-F), with the use of standard ... See full document
7
High Resolution Genotyping of Campylobacter Species by Use of PCR and High Throughput Mass Spectrometry
... RESULTS MLST strain typing on the T5000 platform and clonal com- plex association for primer selection. The assay for Campy- lobacter on the T5000 platform incorporates two primer pairs that unambiguously distinguish C. ... See full document
6
Development of Real Time PCR Assays for Genotyping of Chlamydia trachomatis
... nested real-time PCR (NRT-PCR) for the genotyping of Chlamydia trachomatis and used it specifically for the typing of either eight genovars from D to K or three genovars of ... See full document
5
Quantification of Leishmania infantum DNA by a Real Time PCR Assay with High Sensitivity
... tional PCR, respectively. At the time of diagnosis, parasitemia could vary by a wide range (32 to 188,700 parasites/ml, with a median of 837 parasites/ml), while in bone marrow, parasite load was more than ... See full document
7
High Throughput Real Time Reverse Transcription PCR Quantitation of Hepatitis C Virus RNA
... RESULTS The concentration of HCV RNA in the first reference stan- dard was calculated from its specific activity measured on a scintillation counter. The number of RNA molecules synthe- sized was 10 9 copies per ml. ... See full document
6
Real time pcr for genotyping of hepatitis c virus: evaluation and application
... a real time PCR HCV genotyping assay against a nested RT ...that real time PCR is feasible, easier and more sensitive than the nested RT ...RT PCR in 2 samples and ... See full document
5
Related subjects